A5078242157- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Synthesis and biological activity
- Quinazolinone synthesis and applications
- Metal complexes synthesis and properties
- Porphyrin and Phthalocyanine Chemistry
- Ubiquitin and proteasome pathways
- Photochromic and Fluorescence Chemistry
- Crystallography and molecular interactions
- Molecular Sensors and Ion Detection
- Microtubule and mitosis dynamics
- Cancer Mechanisms and Therapy
- Protein Kinase Regulation and GTPase Signaling
- Synthesis and Characterization of Heterocyclic Compounds
- Cancer therapeutics and mechanisms
- Magnetism in coordination complexes
- Metal-Organic Frameworks: Synthesis and Applications
- Ferrocene Chemistry and Applications
- PARP inhibition in cancer therapy
- Heat shock proteins research
- Protein Tyrosine Phosphatases
- Cell death mechanisms and regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Universitat de Barcelona
2015-2024
University of Aveiro
2011-2023
University Medical Center Hamburg-Eppendorf
2021
Universität Hamburg
2021
IPO Porto
2021
Weatherford College
2021
Faculty (United Kingdom)
2017-2018
Bellvitge University Hospital
2018
Abstract The photoactivation of potential anticancer metal complexes is a hot topic current research as it may lead to the development more selective drugs. Photoactivated chemotherapy (PACT) with coordination compounds usually based on (photo)chemical reaction taking place at center. Herein, new strategy exploited that consists “photomodifying” ligand coordinated ions. Platinum(II) from photoswitchable 1,2‐dithienylethene‐containing ligands have been prepared, which exhibit two...
Lung cancer is a serious health problem and the leading cause of death worldwide. The standard use cell lines as in vitro pre-clinical models to study molecular mechanisms that drive tumorigenesis access drug sensitivity/effectiveness undisputable importance. Label-free mass spectrometry bioinformatics were employed proteomic profiles two representative lung unravel specific biological processes. Adenocarcinoma A549 cells enriched proteins related cellular respiration, ubiquitination,...
In the present study, potential anti-neoplastic properties of a series ruthenium half-sandwich complexes formula [Ru(η6-arene)Cl2(PR1R2(1-pyrenyl))] (η6-arene = p-cymene and R1 R2 methyl for 1; η6-arene methylbenzoate 2; phenyl 3; 4; p-cymene, 5; methylbenzoate, 6) have been investigated. The six structurally related organoruthenium(II) compounds prepared in good yields fully characterized; X-ray structures three them, i.e., 1, 2, 4, were determined. Although piano-stool contain large...
Abstract Lung cancer has become the leading killer worldwide, due to late diagnosis and lack of efficient anticancer drugs. We have recently described novel natural-derived tambjamine analogues that are potent anion transporters capable disrupting cellular ion balance, inducing acidification cytosol hyperpolarization plasma membranes. Although these were able compromise cell survival, their molecular mechanism action remains largely unknown. Herein we characterize responses induced by highly...
Lung cancer is the leading cause of cancer-related deaths worldwide; hence novel treatments for this malignancy are eagerly needed. Since natural-based compounds represent a rich source chemical entities in drug discovery, we have focused our attention on tambjamines, natural isolated from marine invertebrates that shown diverse pharmacological activities. Based these structures, recently identified indole-based tambjamine analog 21 (T21) as promising antitumor agent, which modulates...
Supramolecular interactions prevail over DNA-cleaving abilities in the cytotoxicity behaviour of terpyridine-based copper(<sc>ii</sc>) complexes.
The development of photoactivatable metal complexes with potential anticancer properties is a topical area current investigation. Photoactivated chemotherapy using coordination compounds typically based on photochemical processes occurring at the center. In present study, an innovative approach applied that takes advantage remarkable diarylethenes. Following proof-of-concept study two complexes, namely, C1 and C2, series additional platinum(II) from dithienylcyclopentene-based ligands was...
Infertility is a clinical disorder affecting approximately 15% of reproductive-aged couples worldwide. Recently, the influence oxidative stress (OS) in decreased semen quality has been discussed. OS corresponds to an imbalance between oxidants and antioxidants defenses, present organism. High levels reactive oxygen species (ROS) damage biomolecules sperm cells may lead loss membrane integrity, DNA fragmentation or even death by apoptosis. This study aimed evaluate correlation human clinic...
The serine/threonine protein phosphatase 1 (PP1) inhibitors PPP1R2, PPP1R7, and PPP1R11 are evolutionarily ancient highly conserved proteins. Four PP1 isoforms, PP1α, PP1β, PP1γ1, PP1γ2, exist; three of them except PP1γ2 ubiquitous. fact that isoform is present only in mammalian testis sperm led to the notion isoform-specific regulators for may be responsible its function. In this report, we studied these inhibitors, R7, R11, determine their spatial temporal expression regulatory functions...
Abstract The photoactivation of potential anticancer metal complexes is a hot topic current research as it may lead to the development more selective drugs. Photoactivated chemotherapy (PACT) with coordination compounds usually based on (photo)chemical reaction taking place at center. Herein, new strategy exploited that consists “photomodifying” ligand coordinated ions. Platinum(II) from photoswitchable 1,2‐dithienylethene‐containing ligands have been prepared, which exhibit two...
Protein Ser/Thr Phosphatase PPP1CC2 is an alternatively spliced isoform of PPP1C that highly enriched in testis and selectively expressed sperm. Addition the phosphatase inhibitor toxins okadaic acid or calyculin A to caput caudal sperm triggers stimulates motility, respectively. Thus, endogenous mechanisms inhibition are fundamental for controlling function should be characterized. Preliminary results have shown a protein activity resembling PPP1R2 bovine primate spermatozoa.Here we show...
Phosphorylation is a major regulatory mechanism in eukaryotic cells performed by the concerted actions of kinases and phosphatases (PPs). Protein phosphorylation has long been relevant to sperm physiology, from acquisition motility epididymis capacitation female reproductive tract. While precise involved regulation have studied for decades, PPs only recently received research interest. Tyrosine was first implicated several sperm-related functions, oocyte binding. Only afterwards, 1996,...
Summary Reversible phosphorylation plays an important role as a mechanism of intracellular control in eukaryotes. PPP1, major eukaryotic Ser/Thr-protein phosphatase, acquires its specificity by interacting with different protein regulators, also known PPP1 proteins (PIPs). In the present work we characterized physiologically relevant PIP testis. Using yeast two-hybrid screen human testis cDNA library, identified novel PPP1CC2 isoform, T-complex expressed 1 domain containing 4 (TCTEX1D4) that...
Abstract Background Pseudogenes are traditionally considered “dead” genes, therefore lacking biological functions. This view has however been challenged during the last decade. is case of Protein phosphatase 1 regulatory subunit 2 (PPP1R2) or inhibitor-2 gene family, for which several incomplete copies exist scattered throughout genome. Results In this study, pseudogenization process PPP1R2 was analyzed. Ten PPP1R2-related pseudogenes (PPP1R2P1-P10), highly similar to PPP1R2, were retrieved...
Reaction of cis-[PtCl2(DMSO)2] with the ligand 4-methyl-2-N-(2-pyridylmethylene)aminophenol (Hpyrimol, LH) in methanol at room temperature produces complexes [PtCl2LH] or [PtClL], under acidic basic conditions, respectively. The two platinum compounds exhibit distinct DNA-interacting properties, showing a higher affinity for biomolecules. Furthermore, [PtClL] is clearly more cytotoxic than three different cancer cell lines. Solution studies reveal occurrence intricate solvation processes...