A5063056437- Fungal and yeast genetics research
- Sperm and Testicular Function
- CRISPR and Genetic Engineering
- Microtubule and mitosis dynamics
- Reproductive Biology and Fertility
- MicroRNA in disease regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Photosynthetic Processes and Mechanisms
- Nanoplatforms for cancer theranostics
- Bacteriophages and microbial interactions
- Circular RNAs in diseases
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Trypanosoma species research and implications
- Cervical Cancer and HPV Research
- RNA Interference and Gene Delivery
- Research on Leishmaniasis Studies
- Hepatitis B Virus Studies
- Biosensors and Analytical Detection
- Genomics, phytochemicals, and oxidative stress
- Phosphodiesterase function and regulation
- Oral health in cancer treatment
- Medicinal Plants and Neuroprotection
- RNA Research and Splicing
- SARS-CoV-2 detection and testing
KIIT University
2021-2024
Indian Institute of Chemical Biology
2022-2023
Vanderbilt University
2020-2023
Twist Bioscience (United States)
2023
Tel Aviv University
2022
Footscray Hospital
2020
Western Health
2020
Kent State University
2015-2018
Various health agencies, such as the European Medical Agency (EMA), Centers for Disease Control and Prevention (CDC), World Health Organization (WHO), timely cited upsurge of antibiotic resistance a severe threat to public global economy. Importantly, there is rise in nosocomial infections among covid-19 patients in-hospitalized with delineating disorder. Most are related bacteria residing biofilm, which commonly formed on material surfaces. In biofilms, microcolonies various live syntropy;...
The signaling enzyme glycogen synthase kinase 3 (GSK3) exists as two isoforms-GSK3A and GSK3B. Protein phosphorylation by GSK3 has important roles in several cells. In our past work, we found that both isoforms of are present mouse sperm catalytic activity correlates with motility from species. Here, examined the role Gsk3a male fertility using a targeted gene knockout (KO) approach. mutant mice viable, but have infertility phenotype, while female is unaffected. Testis weights Gsk3a(-/-)...
Abstract Glycogen synthase kinase 3 (GSK3) is a highly conserved protein regulating key cellular functions. Its two isoforms, GSK3α and GSK3β, are encoded by distinct genes. In most tissues the isoforms functionally interchangeable, except in developing embryo where GSK3β essential. One functional allele of either sufficient to maintain normal tissue Both GSK3 present sperm from several species including human, suggested play role epididymal initiation motility. Using genetic approaches, we...
Phosphorylation by the DYRK kinase Pom1 is one of two major signals for proper division site placement in Schizosaccharomyces pombe. phosphorylation F-BAR protein Cdc15 inhibits its membrane and binding, thus inhibiting scaffolding cytokinetic ring preventing mislocalized division.
The emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants made novel diseases (COVID-19) pandemic/endemic/or both more and difficult to manage due increased worry about the efficacy efficiency of present preventative, therapeutic, sensing measures. To deal with these unexpected circumstances, development nano-systems tuneable optical, electrical, magnetic, morphological properties can lead research needed for (1) COVID-19 infection (anti-microbial systems against...
The F-BAR protein Cdc15 is essential for cytokinesis in Schizosaccharomyces pombe and plays a key role attaching the cytokinetic ring (CR) to plasma membrane (PM). Cdc15's abilities bind oligomerize via its domain are inhibited by phosphorylation of intrinsically disordered region (IDR). Multiple cell polarity kinases regulate IDR phosphostate, these DYRK kinase Pom1 sites on have been shown vivo prevent CR formation at tips. Here, we compared ability control phosphostate cortical...
The serine/threonine protein phosphatase 1 (PP1) inhibitors PPP1R2, PPP1R7, and PPP1R11 are evolutionarily ancient highly conserved proteins. Four PP1 isoforms, PP1α, PP1β, PP1γ1, PP1γ2, exist; three of them except PP1γ2 ubiquitous. fact that isoform is present only in mammalian testis sperm led to the notion isoform-specific regulators for may be responsible its function. In this report, we studied these inhibitors, R7, R11, determine their spatial temporal expression regulatory functions...
The multifaceted glycogen synthase kinase (GSK3) has an essential role in sperm and male fertility. Since cyclic AMP (cAMP) plays important function, we investigated whether GSK3 cAMP pathways may be interrelated. We used soluble adenylyl cyclase (sAC) knockout mice pharmacological modulators to examine this relationship. Intracellular levels were found significantly lower lacking GSK3α or GSK3β. A similar outcome was observed when cells treated with SB216763, a inhibitor. This reduction of...
The F-BAR protein Cdc15 mediates attachment of the cytokinetic ring (CR) to plasma membrane and is essential for cytokinesis in Schizosaccharomyces pombe. While its N-terminal domain responsible oligomerization binding, C-terminal SH3 binds other partners at a distance from membrane. We previously demonstrated that formin Cdc12, through an motif, directly cytosolic face domain. Here, we show paxillin-like Pxl1, which important CR stability, contains motif highly related also directly....