A5020720075- Advanced Glycation End Products research
- Immune Response and Inflammation
- S100 Proteins and Annexins
- Neuroinflammation and Neurodegeneration Mechanisms
- Glycosylation and Glycoproteins Research
- Mast cells and histamine
- Immune Cell Function and Interaction
- Proteoglycans and glycosaminoglycans research
- Inflammatory mediators and NSAID effects
- Natural Antidiabetic Agents Studies
- Alcohol Consumption and Health Effects
- Plant tissue culture and regeneration
- Sepsis Diagnosis and Treatment
- Biochemical effects in animals
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Inflammasome and immune disorders
- Cell Adhesion Molecules Research
- Neurogenesis and neuroplasticity mechanisms
- Acute Ischemic Stroke Management
- Immunotherapy and Immune Responses
- Immunodeficiency and Autoimmune Disorders
- Neuroscience and Neuropharmacology Research
- Immune cells in cancer
- Monoclonal and Polyclonal Antibodies Research
- Organ Transplantation Techniques and Outcomes
Shujitsu University
2016-2025
Pharmac
2019-2023
Okayama University
2003-2023
Kindai University
1985-2023
Toshiba (Japan)
2019
Tongji Hospital
2017
Huazhong University of Science and Technology
2017
Japanese Red Cross Nagoya Daiichi Hospital
2013
Aichi Cancer Center
2013
Morpho (United States)
2011
Abstract In recent electron microscope studies of the corpus callosum in 60–80 gm male rats, some cells were identified as microglia by staining with weak silver carbonate method del Rio‐Hortega, and others astrocytes gold chloride sublimate Ramón y Cajal. present work, which did not belong one these two groups examined light using semithin sections stained toluidine blue thin uranyl‐lead. These make up a large, somewhat heterogeneous group, but they have few common features: regular...
Abstract The staining of half‐micron thick Epon sections with toluidine blue provides a reliable method for the identification glial cells. diagnostic features these cells observed in corpus callosum one‐month‐old rats are as follows: (1) Astrocytes have very pale nucleus and cytoplasm; nuclear envelope is sharply outlined by thin chromatin lining occasional beads; (2) Microglia small which large, dark masses contrast nucleoplasm; round or elongated, may be somewhat angular. pericytes,...
The high mobility group box-1 (HMGB1), originally identified as an architectural nuclear protein, exhibits inflammatory cytokine-like activity in the extracellular space. Here we show that treatment with neutralizing anti-HMGB1 monoclonal antibody (mAb; 200 microg, twice) remarkably ameliorated brain infarction induced by 2-h occlusion of middle cerebral artery rats, even when mAb was administered after start reperfusion. Consistent 90% reduction infarct size, accompanying neurological...
Background and Purpose— High mobility group box-1 (HMGB1) exhibits inflammatory cytokine-like activity in the extracellular space. We previously demonstrated that intravenous injection of anti-HMGB1 monoclonal antibody (mAb) remarkably ameliorated brain infarction induced by middle cerebral artery occlusion rats. In present study, we focused on protective effects mAb marked translocation HMGB1 brain, disruption blood–brain barrier (BBB), resultant edema. Methods— Middle rat was used as...
Abstract Objective: High mobility group box‐1 (HMGB1) plays an important role in triggering inflammatory responses many types of diseases. In this study, we examined the involvement HMGB1 traumatic brain injury (TBI) and evaluated ability intravenously administered neutralizing anti‐HMGB1 monoclonal antibody (mAb) to attenuate injury. Methods: Traumatic was induced rats or mice by fluid percussion. Anti‐HMGB1 mAb control after TBI. Results: remarkably inhibited percussion‐induced edema rats,...
In a previous paper Cutz, Bryan et al. showed that in rabbits after repetitive lung lavage high-frequency oscillatory ventilation maintained excellent gas exchange and did not cause hyaline membrane formation (J. Appl. Physiol. 55: 131–138, 1983). contrast, conventional mechanical had poor extensive we attributed these differences to barotrauma. However, completely overlooked the large number of granulocytes damaged lung. To investigate this using same model have used on two groups rabbits,...
Abstract The gold chloride sublimate method of Ramón y Cajal was used to stain astrocytes in the corpus callosum 60–80 g male rats. opacity metal‐stained electron beam allowed their study microscope. It thus found that metal deposits are absent from nucleus but present over two cytoplasmic structures: bundles filaments and dense bodies. Once features had been observed stained preparations, it became possible identify them sections for microscopy by routine uranyl‐lead sequence. presence...
High-mobility group box protein 1 (HMGB1) is a DNA-binding and cytokine highly expressed in atherosclerotic lesions, but its pathophysiological role atherosclerosis unknown. We investigated the development of ApoE-/- mice.Apolipoprotein E-deficient (ApoE-/-) mice fed high-fat diet were administered monoclonal anti-HMGB1 neutralizing antibody, effects on lesion size, immune cell accumulation, proinflammatory mediators assessed using Oil Red O, immunohistochemistry, real-time polymerase chain...
Inflammatory processes in brain tissue have been described human epilepsy of various etiologies and experimental models seizures. High mobility group box-1 (HMGB1) is now recognized as representative damage-associated molecular patterns (DAMPs). In the present study, we focused on whether anti-HMGB1 antibody treatment could relieve status epilepticus- triggered BBB breakdown inflammation response addition to seizure behavior itself. Pilocarpine methyl-scopolamine were used establish acute...
Abstract As one of the most lethal stroke subtypes, intracerebral hemorrhage (ICH) is acknowledged as a serious clinical problem lacking effective treatment. Available evidence from preclinical and studies suggests that inflammatory mechanisms are involved in progression ICH-induced secondary brain injury. High mobility group box-1 (HMGB1) ubiquitous abundant nonhistone DNA-binding protein, also an important proinflammatory molecule once released into extracellular space nuclei. Here, we...
This paper reports the identification of lyn gene product, a member src-related family protein-tyrosine kinases, and its expression in hematopoietic cells. A lyn-specific sequence (Arg-25 to Ala-119 protein) was expressed Escherichia coli as fusion protein with beta-galactosidase. Antiserum raised against immunoprecipitated 56-kDa from human B lymphocytes. Incubation immunoprecipitate [gamma-32P]ATP resulted phosphorylation this at tyrosine residues. Immunohistological immunoblotting...
Sepsis is a major cause of death worldwide. We show that plasma protein histidine-rich glycoprotein (HRG) was decreased significantly in septic mice with cecal ligation and puncture (CLP) supplementary treatment exogenous HRG improved survival, strong inhibition tight attachment neutrophils to pulmonary vasculatures, subsequent immunothrombosis, DIC state, lung inflammation, hypercytokinemia, activation vascular endothelial cells (VECs). In contrast, knockdown by siRNA exacerbated lethality....
Abstract Although delayed cerebral vasospasm (DCV) following subarachnoid hemorrhage (SAH) is closely related to the progression of brain damage, little known about molecular mechanism underlying its development. High mobility group box-1 (HMGB1) plays an important role as initial inflammatory mediator in SAH. In this study, SAH rat model was employed evaluate effects anti-HMGB1 monoclonal antibody (mAb) on DCV after A vasoconstriction basilar artery (BA) associated with a reduction nuclear...
M2 macrophage promotes pathologic angiogenesis through a release of pro-angiogenic mediators or the direct cell-cell interaction with endothelium in micromilieu several chronic inflammatory diseases including rheumatoid arthritis and cancer where interleukin (IL)-18 also contributes to excessive angiogenesis. However, detailed mechanism remains unclear. The aim this study is investigate by which macrophages containing IL-18 induce vitro experimental model using mouse macrophage-like cell...
Background and Purpose Microvascular barrier breakdown is a hallmark of sepsis that associated with sepsis‐induced multiorgan failure. Histidine‐rich glycoprotein (HRG) 75‐kDa plasma protein was demonstrated to improve the survival septic mice through regulation cell shape, spontaneous ROS production in neutrophils, adhesion neutrophils vascular endothelial cells. We investigated HRG's role LPS/TNF‐α‐induced dysfunction cells vitro vivo possible mechanism, clarify definitive roles HRG...
Introduction Tumor necrosis factor-α (TNF-α)-induced angiogenesis plays a critical role in tumor progression and metastasis, making it an important therapeutic target cancer treatment. Suppressing can effectively limit growth metastasis. However, despite advancements understanding angiogenic pathways, effective strategies to inhibit TNF-α-mediated remain limited. Methods This study investigates the antiangiogenic effects of histidine-rich glycoprotein (HRG), multifunctional plasma protein...
The radioactive and thermal effects of radon hot spring were biochemically compared under a sauna room or conditions with similar chemical component, using the parameters that are closely involved in clinic for therapy. results showed therapy enhanced antioxidation functions, such as activities superoxide dismutase (SOD) catalase, which inhibit lipid peroxidation total cholesterol produced body. Moreover concanavalin A (ConA)-induced mitogen response increased percentage CD4 positive cells,...
We have developed a new decalcifying technique for use in light and electron microscopy studies utilizing immunohistochemical staining of calcified tissues. Specimens containing tissues can be adequately decalcified at pH 7.1-7.4 temperature -5 degrees C, without freezing, by solution EDTA, sodium hydroxide, glycerol. In this study, Leu-2a, Leu-3a, Leu-4, Leu-7, Leu-12, Leu-14, Leu-M1, Leu-M2, Leu-M3, HLA-DR-positive cells destructive lesions bone from patients with rheumatoid arthritis...