A5058647472- Trypanosoma species research and implications
- Acoustic Wave Resonator Technologies
- Advanced Biosensing Techniques and Applications
- Research on Leishmaniasis Studies
- DNA Repair Mechanisms
- Biosensors and Analytical Detection
- Synthesis and Biological Evaluation
- Antifungal resistance and susceptibility
- Cardiomyopathy and Myosin Studies
- Cardiac pacing and defibrillation studies
- Biochemical and Molecular Research
- Monoclonal and Polyclonal Antibodies Research
- CRISPR and Genetic Engineering
- Cardiovascular Function and Risk Factors
- Disaster Response and Management
- Escherichia coli research studies
- Parasites and Host Interactions
- Cardiac tumors and thrombi
- Advanced Radiotherapy Techniques
- Cancer therapeutics and mechanisms
- Diagnosis and treatment of tuberculosis
- Neonatal and Maternal Infections
- Herbal Medicine Research Studies
- Infectious Disease Case Reports and Treatments
- Ocular Infections and Treatments
Universidad de Antioquia
2024
Instituto Butantan
2017-2020
Toxicologie, Pharmacologie et Signalisation Cellulaire
2018
EIA University
2013-2016
Clínica CES
2016
University of Puerto Rico at Río Piedras
2012
Abstract Trypanosomatids are the etiologic agents of various infectious diseases in humans. They diverged early during eukaryotic evolution and have attracted attention as peculiar models for evolutionary comparative studies. Here, we show a meticulous study comparing incorporation detection thymidine analogs BrdU EdU Leishmania amazonensis , Trypanosoma brucei cruzi to monitor their DNA replication. We used BrdU‐ EdU‐incorporated parasites with respective standard approaches: indirect...
Human placental explants (HPEs) culture has generated significant interest as a valuable in vitro model for studying tissue functions response to adverse conditions, such fluctuations oxygen levels, nutrient availability, exposure pathogenic microorganisms, and toxic compounds. HPEs offers the advantage of replicating intricate microenvironment cell-to-cell communication involved this critical transient organ. Although conditions have been extensively discussed, protocol assessing viability...
Abstract One of the most important mechanisms for repairing double-strand breaks (DSBs) in model eukaryotes is homologous recombination (HR). Although genes involved HR have been found Trypanosoma brucei and studies identified some proteins that participate this pathway, recruitment kinetics machinery onto DNA during DSB repair not clearly elucidated organism. Using immunofluorescence, protein DNA-bound assays, content analysis, we established pathway response to DSBs generated by ionizing...
Abstract Detection of genetic exchange has been a limiting factor to deepen the knowledge on mechanisms by which Trypanosoma cruzi is able generate progeny and diversity. Here we show that incorporation halogenated thymidine analogues, followed immunostaining, reliable method not only detect T. fused-cell hybrids, but also quantify their percentage in populations this parasite. Through approach, were hybrids clones CL Brener Y. Given increased detection naturally-occurring hybrid strain,...
Abstract The co-synthesis of DNA and RNA potentially generates conflicts between replication transcription, which can lead to genomic instability. In trypanosomatids, eukaryotic parasites that perform polycistronic this phenomenon its consequences are still little studied. Here, we showed the number constitutive origins mapped in Trypanosoma brucei genome is less than minimum required complete within S-phase duration. By development a mechanistic model considering replication-transcription...
DNA double-strand breaks (DSBs) are among the most deleterious lesions that threaten genome integrity. To address DSBs, eukaryotic cells of model organisms have evolved a complex network cellular pathways able to detect damage, activate checkpoint response delay cell cycle progression, recruit proper repair machinery, and resume once damage is repaired. Cell checkpoints primarily regulated by apical kinases ATR ATM, which conserved throughout kingdom. Trypanosoma brucei divergent pathogenic...
Trypanosoma cruzi is a protozoan parasite belonging to the Trypanosomatidae family. Although trypanosomatids multiply predominantly by clonal generation, presence of DNA exchange in some them has been puzzling researchers over years, mainly because it may represent novel form that these organisms use gain variability. Analysis Exchange using Thymidine Analogs (ADExTA) method allows vitro detection and measurement rates exchange, particularly trypanosomatid cells, rapid simple manner indirect...
The progress in the development of a piezoelectric immunosensor for detection secretion protein (38 kDa) from Mycobacterium tuberculosis are described. A high affinity and specificity monoclonal antibody was obtained recombinant form target protein. This immunoreagent employed competitive ELISA, as previous diagnostic test reference assay characterization future biosensor. ELISA limit 14 ng/mL 38kDa antigen. first experimental approach to undertaken using bovine serum albumin (BSA) model...
The selection and procurance of the immunoreagents used in immunoassay biofunctionalisation transducer strategy, are a key piezoelectric immunosensor development for detection tuberculosis (TB). Many have reported use 38kDa protein antigen (Ag38kDa) from Mycobacterium tuberculosis ( Mtb ) such as good biomarker TB disease compliance with physical biochemical characteristics to be immobilized by self-assembled monolayers (SAMs), gold electrode piezoelectrics crystals surfaces. A developed...
Monoclonal antibodies have been applied in biomedical research for diagnosis of various diseases and the treatment increasing prevalence such as tuberculosis. There is a need to develop systems capable detecting tuberculosis-related biomarkers that can provide quick begin immediately. Thanks advances detection, biosensors technologies are emerging good alternative clinical applications. The aim this work was piezoelectric immunosensor rapid efficient tuberculosis detection. To purpose, 12...
ABSTRACT The cosynthesis of DNA and RNA potentially generates conflicts between replication transcription, which can lead to genomic instability. In trypanosomatids, eukaryotic parasites that perform polycistronic this phenomenon its consequences have not yet been investigated. Here, using equations computational analysis we demonstrated the number constitutive origins mapped in Trypanosoma brucei genome is close minimum required complete within S phase duration. However, taking into account...
La tuberculosis es un problema de salud pública que afecta a millones personas, siendo la cuarta causa muerte por enfermedad infecciosa en el mundo. En su más reciente reporte, Organización Mundial Salud (OMS) menciona infección tuberculosa curable con tratamiento adecuado; sin embargo, una necesidad detección precoz casos y mejora del diagnóstico como medida control. esta revisión se presenta recopilación los métodos desde tradicionales hasta las nuevas alternativas desarrollo. Como...