Bacterial LPS (endotoxin) has been implicated in the pathogenesis of acute liver disease through its induction proinflammatory cytokine TNF-α. TNF-α is a key determinant outcome well-established mouse model failure during septic shock. One possible mechanism for regulating expression control protein elongation translation, which would allow rapid cell adaptation to physiological changes. However, regulation translational poorly understood. We found that p38γ/δ MAPK proteins required nascent...

Bordetella spp. form biofilms in the mouse nasopharynx, thereby providing a potential mechanism for establishing chronic infections humans and animals. Filamentous hemagglutinin (FHA) is major virulence factor of B. pertussis, causative agent highly transmissible infectious disease, pertussis. In this study, we dissected role FHA distinct biofilm developmental stages pertussis on abiotic substrates respiratory tract by employing murine model biofilms. Our results show that lack reduced...

Pertussis, a serious infectious disease of the respiratory tract caused by Bordetella pertussis, is reemerging in vaccinated populations. Efforts to curtail this are hampered limited insight into basis protective immunity. Opsonophagocytosis was recently found play central role cellular bactericidal activity against B. pertussis. In present study, we studied specificity opsonic antibodies. Anti-pertactin antibodies, but not anti-pertussis toxin, anti-fimbriae, or anti-filamentous...

Although Bordetella pertussis has been observed to survive inside macrophages, its ability resist or evade degradation in phagolysosomes not defined. We here investigated the trafficking of B. upon entry into human macrophages. During first hours following phagocytosis, a high percentage bacteria were destroyed within acidic compartments positive for lysosome-associated membrane proteins (LAMP). However, roughly one-fourth taken up this initial killing event, remaining nonacidic...

Immunoglobulin G (IgG)-mediated phagocytosis by polymorphonuclear leukocytes (PMNL) constitutes the main defense against Streptococcus pneumoniae. Two leukocyte IgG receptors, FcgammaRIIa and FcgammaRIIIb, are constitutively expressed on PMNL. Blocking experiments showed is crucial for opsonophagocytosis of serum-opsonized S. The biallelic, genetically determined polymorphism (FcgammaRIIa-R131 vs. IIa-H131) determines capacity IgG2-mediated via this receptor. Comparative studies with PMNL...

Bordetella pertussis is the causative agent of pertussis, aka whooping cough. Although generally considered an extracellular pathogen, this bacterium has been found inside respiratory epithelial cells, which might represent a survival strategy host. Relatively little known, however, about mechanism internalization and fate B. epithelia. We show here that able to enter those cells by dependent on microtubule assembly, lipid raft integrity, activation tyrosine-kinase-mediated signaling. Once...

In the present work we investigated importance of Raf/MEK/ERK signalling pathway in multiplication arenavirus Junín (JUNV) monkey and human cell cultures. We established that JUNV induces a biphasic activation ERK proved specific inhibitor pathway, U0126, impairs viral replication. Furthermore, U0126 exerted inhibitory action against arenaviruses Tacaribe Pichinde. Moreover, treatment with known activators such as phorbol 12-myristate 13-acetate serum increased yields whereas silencing by...

Abstract The relevance of specific Abs for the induction cellular effector functions against Bordetella pertussis was studied. IgG-opsonized B. efficiently phagocytosed by human polymorphonuclear leukocytes (PMN). This process mediated PMN IgG receptors, FcγRIIa (CD32) and FcγRIIIb (CD16), working synergistically. Furthermore, these FcγR triggered efficient respiratory burst activity transfer to lysosomal compartments, ultimately resulting in reduced bacterial viability. Bacteria opsonized...

Bordetella pertussis, the etiological agent of whooping cough, still causes outbreaks. We recently found evidence that B. pertussis can survive and even replicate inside human macrophages, indicating this host cell might serve as a niche for persistence. In work, we examined interaction with monocyte line (THP-1) differentiates into macrophages in culture order to investigate response infection mechanisms promote intracellular survival. To end, investigated expression profile selected number...

B. parapertussis is a whooping cough etiological agent with the ability to evade immune response induced by pertussis vaccines. We previously demonstrated that in absence of opsonic antibodies hampers phagocytosis neutrophils and macrophages and, when phagocytosed, blocks intracellular killing interfering phagolysosomal fusion. But can kill and/or immobilize extracellular bacteria through non-phagocytic mechanisms such as degranulation neutrophil traps (NETs). In this study we also has...

Specific anti—capsular polysaccharide IgG is believed to be important for protection against infection by Streptococcus pneumoniae. Significant IgA responses have been observed after vaccination with pneumococcal vaccines, but the role of this isotype in anti-pneumococcal host defense unclear. Here, it shown that purified serum specific capsular polysaccharides can initiate efficient cellular effector functions, such as phagocytosis, via interaction myeloid receptor, FcαRI (CD89). The...

Regulation of gene expression in response to local iron concentration is commonly observed bacterial pathogens that face this nutrient limitation during host infection. In study, a proteomic approach was used analyze the differential protein Bordetella pertussis under limitation. Whole cell lysates (WCL) and outer membrane fractions bacteria grown either iron-starvation or iron-excess conditions were analyzed by two-dimensional (2-D) gel electrophoresis. Statistical analysis revealed 36...

Although the prevalence of Bordetella parapertussis varies dramatically among studies in different populations with vaccination regimens, there is broad agreement that whooping cough vaccines, composed only B. pertussis antigens, provide little if any protection against parapertussis. In C57BL/6 mice, a whole-cell vaccine (wP) provided modest parapertussis, which was dependent on IFN-γ. The wP much more protective an isogenic strain lacking O-antigen than its wild-type counterpart. inhibited...

Bordetella pertussis, a strictly human re-emerging pathogen and the causative agent of whooping cough, exploits broad variety virulence factors to establish efficient infection. Here, we used RNA sequencing analyse changes in gene expression profiles THP-1 macrophages resulting from B. pertussis In parallel, attempted determine intracellular pertussis-specific transcriptomic interaction with macrophages. Our analysis revealed that global are extensively rewired 6 h post-infection. Among...

ABSTRACT Pertussis is a severe respiratory disease caused by infection with the bacterial pathogen Bordetella pertussis . The affects individuals of all ages but particularly and sometimes fatal in unvaccinated young infants. Other species cause diseases humans, animals, birds. Scientific, clinical, public health, vaccine company, regulatory agency experts on these pathogens gathered Buenos Aires, Argentina from 5 to 8 April 2016 for 11th International Symposium discuss recent advances our...

Attachment to epithelial cells in the respiratory tract is a key event Bordetella pertussis colonization. Filamentous haemagglutinin (FHA) an important virulence factor mediating adhesion host cells. In this study, relevance of interaction between FHA and adenylate cyclase toxin (ACT) during bacterial attachment was investigated. Mutants lacking either or ACT showed significantly decreased adherence The use several ACT-specific monoclonal antibodies antiserum that decrease strains expression...