A5005317457- Bacterial Infections and Vaccines
- Bacterial Genetics and Biotechnology
- Pneumonia and Respiratory Infections
- Bacterial biofilms and quorum sensing
- RNA and protein synthesis mechanisms
- Influenza Virus Research Studies
- Infective Endocarditis Diagnosis and Management
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Genomics and Phylogenetic Studies
- Antimicrobial Peptides and Activities
- Yersinia bacterium, plague, ectoparasites research
- Infectious Encephalopathies and Encephalitis
- Immune Response and Inflammation
- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Carbohydrate Chemistry and Synthesis
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Antibiotic Resistance in Bacteria
- Vector-borne infectious diseases
- Viral gastroenteritis research and epidemiology
- Herpesvirus Infections and Treatments
- Peripheral Neuropathies and Disorders
- Porphyrin Metabolism and Disorders
The Ohio State University
2018-2024
The Ohio State University Wexner Medical Center
2017-2023
Wake Forest University
2008-2018
Ohio University
2018
Atrium Health Wake Forest Baptist
2013
Forest Institute
2011
Institute of Molecular Genetics
2006-2010
North Carolina State University
2007
University of California, Los Angeles
2002-2004
Howard Hughes Medical Institute
2004
Host-pathogen interactions are often driven by mechanisms that promote genetic variability. We have identified a group of temperate bacteriophages generate diversity in gene, designated mtd (major tropism determinant), which specifies for receptor molecules on host Bordetella species. Tropism switching is the result template-dependent, reverse transcriptase-mediated process introduces nucleotide substitutions at defined locations within mtd. This cassette-based mechanism capable providing...
Bacteria form complex and highly elaborate surface adherent communities known as biofilms which are held together by a self-produced extracellular matrix. We have previously shown that adopting biofilm mode of existence in vivo, the Gram negative bacterial pathogens Bordetella bronchiseptica pertussis able to efficiently colonize persist mammalian respiratory tract. In general, matrix includes polysaccharides, proteins DNA (eDNA). this report, we investigated function development. show is...
A homolog of the multiple-stress-responsive transcription factor sigmaB Bacillus subtilis was predicted from DNA sequence analysis a region Staphylococcus aureus chromosome. hybrid between coding first 11 amino acids gene 10 leader peptide phage T7 (T7.Tag) and putative sigB S. constructed cloned into Escherichia coli BL21(DE3)pLysS for overexpression promoter. homogeneous preparation overproduced protein obtained by affinity chromatography with T7.Tag monoclonal antibody coupled to agarose....
Two analogues of the marine natural product bromoageliferin have been synthesized and subsequently assayed for ability to inhibit formation Pseudomonas aeruginosa biofilms. Both compounds inhibited strains PAO1 PA14 form biofilms; however, they displayed differential toxicity planktonic bacteria. The cis analogue, CAGE, was found possess greater than trans TAGE. These no structural homology any known inhibitors
Bordetellae are gram-negative bacteria that colonize the respiratory tracts of animals and humans. We others have recently shown these capable living as sessile communities known biofilms on a number abiotic surfaces. During biofilm mode existence, produce one or more extracellular polymeric substances function, in part, to hold cells together surface. There is little information either constituents matrix genetic basis development by Bordetella spp. By utilizing immunoblot assays enzymatic...
ABSTRACT Bordetellae are respiratory pathogens that infect both humans and animals. Bordetella bronchiseptica establishes asymptomatic long-term to life-long infections of animal nasopharynges. While the human pathogen pertussis is etiological agent acute disease whooping cough in infants young children, it now being increasingly isolated from nasopharynges vaccinated adolescents adults who sometimes show milder symptoms, such as prolonged illness. Although has been shown can form biofilms...
Many respiratory pathogens establish persistent infection or a carrier state in the human nasopharynx without overt disease symptoms but presence of these lungs usually results disease. Although anatomy and microenvironments between are different, virulence factor with an organ-specific function colonization is unknown. In contrast to severity pertussis mortality non-vaccinated young children, Bordetella milder prolonged cough vaccinated adolescents adults. Individuals harbouring bacteria...
When Escherichia coli O157:H7 bacteria are added to alfalfa sprouts growing in water, the bind tightly sprouts. In contrast, laboratory K-12 strains of E. do not under similar conditions. The roles lipopolysaccharide (LPS), capsular polysaccharide, and exopolysaccharides binding were examined. An LPS mutant had no effect on pathogenic strain. Cellulose synthase mutants showed a significant reduction binding; colanic acid more severely reduced, by poly-beta-1,6-N-acetylglucosamine (PGA) was...
Bordetella spp. form biofilms in the mouse nasopharynx, thereby providing a potential mechanism for establishing chronic infections humans and animals. Filamentous hemagglutinin (FHA) is major virulence factor of B. pertussis, causative agent highly transmissible infectious disease, pertussis. In this study, we dissected role FHA distinct biofilm developmental stages pertussis on abiotic substrates respiratory tract by employing murine model biofilms. Our results show that lack reduced...
Poly-β(1,6)-N-acetyl-D-glucosamine (PNAG) is a major biofilm component of many pathogenic bacteria. The production, modification, and export PNAG in Escherichia coli Bordetella species require the protein products encoded by pgaABCD operon. PgaB two-domain periplasmic that contains an N-terminal deacetylase domain C-terminal binding critical for export. However, exact function remains unclear. Herein, we show domains bronchiseptica (PgaBBb) E. (PgaBEc) as glycoside hydrolases. These enzymes...
Abstract With the infection rate of Bordetella pertussis at a 60‐year high, there is an urgent need for new anti‐pertussis vaccines. The lipopolysaccharide (LPS) B. attractive antigen vaccine development. presence multiple rare sugars and unusual glycosyl linkages, LPS highly challenging synthetic target. In this work, aided by molecular dynamics simulation modeling, pertussis‐LPS‐like pentasaccharide was chemically synthesized first time. conjugated with powerful carrier, bacteriophage Qβ,...
The BvgAS signal transduction system controls the expression of at least three distinct phenotypic phases that lie along a continuum gene states. Bvg + phase is characterized by adhesins and toxins, whereas − motility in Bordetella bronchiseptica vrg loci pertussis . Bvg‐intermediate (Bvg i ) absence Bvg‐repressed phenotypes, some, but not all, Bvg‐activated virulence factors presence recently discovered set antigens phenotypes are unique to this phase. We report here transcriptional...
A chromosomally encoded znt operon of Staphylococcus aureus consists two consecutive putative genes designated zntR and zntA . The gene encodes a transmembrane protein that facilitates extrusion Zn 2+ Co , whereas the regulatory controls expression operon. was amplified using polymerase chain reaction, cloned into Escherichia coli for overexpression as His‐tagged ZntR purified by Ni ‐affinity column. His‐tag‐free to near homogeneity after digestion with enterokinase. Electrophoretic mobility...
The majority of Bordetella sp. virulence determinants are regulated by the BvgAS signal transduction system. mediates control multiple phenotypic phases and a spectrum gene expression profiles specific to each phase in response incremental changes concentrations environmental signals. Studies highlighting critical role this signaling circuitry infectious cycle have focused on planktonically growing bacterial cells. It is becoming increasingly clear that major mode existence environment...
RNA polymerase (RNAP) isolated from Staphylococcus aureus is deficient in sigma factor and poorly active transcription assays. Based on amino acid sequence homology of the Bacillus subtilis vegetative sigmaA predicted product chromosomally located plaC gene S. aureus, it was hypothesized that could encode factor. We cloned under a T7 promoter overexpressed Escherichia coli strain BL21(DE3)pLysE. The overproduced protein, present inclusion bodies, solubilized with guanidine hydrochloride,...
Bordetella bronchiseptica is a zoonotic respiratory pathogen commonly found in domesticated farm and companion animals, including dogs cats. Here, we report isolation of B. from sputum sample cystic fibrosis patient recently exposed to kitten with an acute illness. Genetic characterization the isolate comparison other isolates human or feline origin strongly suggest that was source infection.
We have used microarray analysis to study the transcriptome of bacterial pathogen Bordetella bronchiseptica over course five time points representing distinct stages biofilm development. The results suggest that B. undergoes a coordinately regulated gene expression program similar developmental process. Expression and subsequent production genes encoding flagella, classical Bvg− phase phenotype, occurs is under tight regulatory control during Using mutational analysis, we demonstrate...
Pertussis, or whooping cough, caused by the obligate human pathogen Bordetella pertussis is undergoing a worldwide resurgence. The majority of studies this are conducted with laboratory-adapted strains which may not be representative species as whole. Biofilm formation B. plays an important role in pathogenesis. We side-by-side comparison biofilm-forming abilities prototype laboratory and currently circulating isolates from two countries different vaccination programs. Compared to reference...
Abstract Contamination of soil by antibiotics and heavy metals originating from hospital facilities has emerged as a major cause for the development resistant microbes. We collected samples surrounding effluent measured resistance bacterial isolates against multiple metals. One strain BMCSI 3 was found to be sensitive all tested antibiotics. However, it many metalloids like cadmium, chromium, copper, mercury, arsenic, others. This motile potentially spore-forming. Whole-genome shotgun...
Introduction Resurgence of pertussis, caused by Bordetella necessitates novel vaccines and vaccination strategies to combat this disease. Alum-adjuvanted acellular pertussis (aPV) delivered intramuscularly reduce bacterial numbers in the lungs immunized animals humans, but do not nasal colonization. Thus, aPV-immunized individuals are sources community transmission. We showed previously that modification a commercial aPV (Boostrix) addition Th1/17 polarizing adjuvant Colonization Factor A...