A5032886929- Adipose Tissue and Metabolism
- Ion channel regulation and function
- Exercise and Physiological Responses
- Cardiac electrophysiology and arrhythmias
- Mitochondrial Function and Pathology
- Muscle metabolism and nutrition
- Adipokines, Inflammation, and Metabolic Diseases
- Muscle Physiology and Disorders
- Physiological and biochemical adaptations
- Bat Biology and Ecology Studies
- Thermoregulation and physiological responses
- Cardiomyopathy and Myosin Studies
- Regulation of Appetite and Obesity
- Diet and metabolism studies
- Biochemical and Molecular Research
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Tuberculosis Research and Epidemiology
- Cardiovascular Effects of Exercise
- Cardiovascular Disease and Adiposity
- Protein Structure and Dynamics
- Cardiovascular Function and Risk Factors
- HIV/AIDS drug development and treatment
- Advanced NMR Techniques and Applications
- Music Therapy and Health
KIIT University
2016-2024
Indian Institute of Technology Bhubaneswar
2022
The Ohio State University
2009-2018
Sanford Burnham Prebys Medical Discovery Institute
2016-2017
Discovery Institute
2016-2017
Cancer Genetics (United States)
2012
The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute
2012
Central Drug Research Institute
2006-2011
Sarcolipin (SLN) is a novel regulator of sarcoplasmic reticulum Ca2+ ATPase (SERCA) in muscle. SLN binding to SERCA uncouples transport from ATP hydrolysis. By this mechanism, promotes the futile cycling SERCA, contributing muscle heat production. We recently showed that plays an important role cold- and diet-induced thermogenesis. However, detailed mechanism how regulates metabolism remains unclear. In study, we used both knockout (Sln−/−) skeletal muscle-specific overexpression (SlnOE)...
The importance of brown adipose tissue as a site nonshivering thermogenesis has been well documented. Emerging studies suggest that skeletal muscle is also an important especially when function lacking. We recently showed sarcolipin (SLN), uncoupler the sarco(endo)plasmic reticulum Ca(2+) ATPase (SERCA) pump, could contribute to heat production in muscle. In this study, we sought understand how loss UCP1 or SLN compensated during cold exposure and whether they are both necessary for...
Skeletal muscle has been suggested as a site of nonshivering thermogenesis (NST) besides brown adipose tissue (BAT). Studies in birds, which do not contain BAT, have demonstrated the importance skeletal muscle-based NST. However, NST mammals remains poorly characterized. We recently reported that sarco/endoplasmic reticulum Ca(2+) cycling and its regulation by SLN can be basis for Because dominant role BAT-mediated rodents, is less obvious. In this study, we investigated whether will become...
The whole-body (tachymetabolic) endothermy seen in modern birds and mammals is long held to have evolved independently each group, a reasonable assumption when it was believed that its earliest appearances arose many millions of years apart. That consistent with current acceptance the non-shivering thermogenesis (NST) component regulatory body heat originates differently group: from skeletal muscle brown adipose tissue (BAT) mammals. However, BAT absent monotremes, marsupials, eutherians,...
There are two well-described thermogenic sites; brown adipose tissue (BAT) and skeletal muscle, which utilize distinct mechanisms of heat production. In BAT, mitochondrial metabolism is the molecular basis generation, while it serves only a secondary role in supplying energy for thermogenesis muscle. Here, we wanted to document changes ultrastructure these types based upon adaptation mild (16°C) severe (4°C) cold mice. When reared at thermoneutrality (29°C), mitochondria both tissues were...
The utrophin-dystrophin deficient (DKO) mouse model has been widely used to understand the progression of Duchenne muscular dystrophy (DMD). However, it is unclear as what extent muscle pathology affects metabolism. Therefore, present study was focused on understanding energy expenditure in whole animal and isolated extensor digitorum longus (EDL) determine changes metabolic enzymes. Our results show that 8 week-old DKO mice consume higher oxygen relative activity levels. Interestingly EDL...
Although the physiological basis of canonical or classical IκB kinase β (IKKβ)–nuclear factor κB (NF-κB) signaling pathway is well established, how alternative NF-κB functions beyond its role in lymphoid development remains unclear. In particular, has been linked with cellular metabolism, but this relationship poorly understood. study, we show that mice deleted for components IKKα RelB have reduced mitochondrial content and function. Conversely, expressing alternative, not classical,...
Sarcolipin (SLN) regulates muscle-based nonshivering thermogenesis and is up-regulated with high-fat feeding (HFF). To investigate whether other thermogenic systems compensate for a lack of Sln to firmly establish SLN as mediator diet-induced (DIT), we measured muscle whole-body energy expenditure in chow- high-fat-fed Sln(-/-) wild-type (WT) mice. Following HFF, resting metabolic rate (VO2, μl/g/s) was increased similarly WT (0.28±0.02 vs. 0.31±0.03) (0.23±0.03 0.35±0.02) mice due...
It is well known that uncoupling protein 1 (UCP1) in brown adipose tissue plays an important role diet-induced thermogenesis. In this study, whether sarcolipin (SLN), a regulator of sarco/endoplasmic reticulum Ca(2+) -ATPase pump muscle, also player thermogenesis was investigated, as loss SLN could be compensated by increased UCP1 expression and vice versa.Age- sex-matched UCP1(-/-) , SLN(-/-) double knockout for both mice maintained C57Bl/6J background were challenged to high-fat diet 12...
Sarcolipin (SLN) is a regulator of sarco/endo plasmic reticulum Ca 2+ -ATPase (SERCA) pump and has been shown to be involved in muscle nonshivering thermogenesis (NST) energy metabolism. Interestingly, SLN expression significantly upregulated both during development several disease states. However, the significance altered patho-physiology not completely understood. We have previously that transgenic over-expression skeletal detrimental, can promote oxidative metabolism exercise capacity. In...
The 6‐kDa early secretory antigenic target (ESAT‐6) and culture filtrate protein‐10 (CFP‐10), expressed from the region of deletion‐1 (RD1) Mycobacterium tuberculosis H37Rv, are known to play a key role in virulence. In this study, we explored thermodynamic biochemical changes associated with formation 1 : heterodimeric complex between ESAT‐6 CFP‐10. Using isothermal titration calorimetry (ITC), precisely determined association constant free energy change for be 2 × 10 7 m −1 −9.95 kcal·mol...