A5058968567- Corneal Surgery and Treatments
- Chronic Myeloid Leukemia Treatments
- Chronic Lymphocytic Leukemia Research
- Silicon Nanostructures and Photoluminescence
- Ocular Disorders and Treatments
- Pleural and Pulmonary Diseases
- Monoclonal and Polyclonal Antibodies Research
- Lymphoma Diagnosis and Treatment
- Corneal surgery and disorders
- Immune Cell Function and Interaction
- Ocular Surface and Contact Lens
- Occupational and environmental lung diseases
- Angiogenesis and VEGF in Cancer
- Retinal Development and Disorders
- Nanoparticles: synthesis and applications
- Nanowire Synthesis and Applications
- Glycosylation and Glycoproteins Research
- Organ and Tissue Transplantation Research
- Cancer Research and Treatments
- Radiomics and Machine Learning in Medical Imaging
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Polymer Surface Interaction Studies
- Galectins and Cancer Biology
- Systemic Lupus Erythematosus Research
Flinders University
2010-2025
South Australian Health and Medical Research Institute
2019-2024
The University of Adelaide
2020-2023
Summary There is currently no biomarker that reliably predicts treatment‐free remission (TFR) in chronic myeloid leukaemia (CML). We characterised effector and suppressor immune responses at the time of tyrosine kinase inhibitor (TKI) cessation patients from CML8 CML10 clinical studies. Natural killer (NK) cells with increased expression activating NK receptors were higher who achieved TFR. was difference proportion CD4 + or CD8 T cells. Furthermore, we found FoxP3 regulatory (T reg)...
Porous silicon (pSi) and poly(L-lactide) (PLLA) both display good biocompatibility tunable degradation behavior, suggesting that composites of materials are suitable candidates as biomaterials for localized drug delivery into the human body. The combination a pliable soft polymeric material with hard inorganic porous high loading capacity may engender improved control over release profiles be beneficial preparation advanced devices biodegradable implants or scaffolds.In this work, three...
Dysregulation of immune-checkpoint receptors has been reported at diagnosis chronic myeloid leukemia (CML), however, their role in the maintenance remission after tyrosine kinase inhibitor (TKI) cessation is unclear. We assessed programmed cell death-1 (PD-1), T-cell immunoglobulin, and mucin-domain containing protein-3 (TIM-3), cytotoxic T-lymphocyte-associated protein-4 (CTLA-4), lymphocyte-activation gene-3 (LAG-3), immunoreceptor with immunoglobulin tyrosine-based inhibitory motif (ITIM)...
The 2021 WHO classification of thoracic tumours recommends grading pleural mesothelioma to aid prognostication. Robustness and morphological characterisation is key its clinical utility, though validation this system has largely been conducted by expert pathologists. We a survey inviting pathologists across range practices expertise grade digitised images 50 epithelioid mesotheliomas that had graded an in pathology. included slides were considered potentially problematic such as small...
The proinflammatory cytokine, tumor necrosis factor-α (TNF-α), is elevated in several diseases such as uveitis, rheumatoid arthritis and non-healing chronic wounds. Adding Infliximab, a chimeric IgG1 monoclonal antibody raised against TNF-α, to wound fluid can neutralise human thereby providing potential therapeutic option for healing. However, avoid the need repeated application clinical setting, protect from hostile environment of wound, suitable delivery vehicles are required. Porous...
Purpose: The species cross-reactivity of the monoclonal antibodies infliximab, bevacizumab, and an anti–VEGF-B antibody, 2H10, in humans rodents was determined. Methods: binding infliximab to human, mouse, rat TNF-α, bevacizumab VEGF-A, 2H10 antibody VEGF-B evaluated by ELISA. sequence TNF-α VEGF-A at sites for were compared. Results: Infliximab bound human but no mouse or detected between 10 pg/mL μg/ml. Sequence comparison site revealed four changes five compared with human. Bevacizumab...
The addition of interferon to tyrosine kinase inhibitors (TKIs), improve deep molecular response (DMR) and potentially treatment-free remission (TFR) rates in chronic-phase chronic myeloid leukaemia (CP-CML) patients is under active investigation. However, the immunobiology this combination poorly understood. We performed a comprehensive longitudinal assessment immunological changes CML treated with nilotinib interferon-alpha (IFN-α) within ALLG CML11 trial (n = 12) or alone 17). demonstrate...
We tested the ability of an antibody fragment with specificity for vascular endothelial growth factor-B (VEGF-B) to regress nascent and established corneal blood vessels in rat.A single chain variable (scFv) VEGF-B was engineered from 2H10 hybridoma. Binding rat, mouse, human confirmed by surface plasmon resonance. Activity anti-VEGF-B scFv on developing assessed following unilateral superficial cautery male female outbred Sprague Dawley rats. Groups (untreated, control scFv-treated, or...
Dysfunction of limbal stem cells or their niche can result in painful, potentially sight-threatening ocular surface disease. We examined the utility surface-modified porous-silicon (pSi) membranes as a scaffold for transfer oral mucosal to eye. Male-origin rat epithelial were grown on pSi coated with collagen-IV and vitronectin, characterised by immunocytochemistry. Scaffolds bearing implanted into normal female rats, close limbus, 8 weeks. Histology, immunohistochemistry multiplex nested...
Purpose: To evaluate the gene transfer of interleukin (IL)-10 cytokine as a treatment modality for prolonging limbal allograft survival in rat model.Materials and methods: Adenoviral (AV) lentiviral (LV) vectors were produced ex vivo into graft tissue prior to orthotopic transplantation. Experimental groups comprised unmodified isografts, allografts, allografts transfected with reporter gene, IL-10. The functional effects transgenes determined by clinical assessment following donor cell...
Sex-based differences in susceptibility have been reported for a number of neovascular ocular diseases. We quantified corneal neovascularization, induced by superficial silver nitrate cautery, male and female inbred albino Sprague-Dawley, Fischer 344, outbred pigmented Hooded Wistar Dark Agouti rats range ages. Corneal area was on haematoxylin-stained flatmounts image analysis. Pro-and anti-angiogenic gene expression measured early the response quantitative real-time polymerase chain...
Vascular endothelial growth factor (VEGF) plays a major role in the development of aberrant neovascularization ocular diseases such as diabetic retinopathy and age-related macular degeneration (ARMD), is an important therapeutic target for these diseases. Monoclonal antibodies specific VEGF are clinical use some patients with ARMD, delivered by intraocular injection. We have shown previously that single chain antibody fragments (scFv) penetrate into eye when applied topically to surface....
Ex vivo-expanded buccal mucosal epithelial (BME) cell transplantation has been used to reconstruct the ocular surface. Methods for enrichment and maintenance of BME progenitor cells in ex vivo cultures may improve outcome transplantation. However, parameter seeding density this context largely neglected. This study investigates how varying influences proliferation differentiation on tissue culture polystyrene (TCPS). The highest activity was seen when were seeded at 5×104 cells/cm2. Both...