A5052697833- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Enzyme Production and Characterization
- Synthesis of Organic Compounds
- Photodynamic Therapy Research Studies
- Polyamine Metabolism and Applications
- Chemical Synthesis and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Biochemical and Molecular Research
- Enzyme Structure and Function
- Lysosomal Storage Disorders Research
- Photoreceptor and optogenetics research
- Molecular Sensors and Ion Detection
- Sulfur-Based Synthesis Techniques
- Porphyrin and Phthalocyanine Chemistry
- Bioactive Compounds and Antitumor Agents
- DNA and Nucleic Acid Chemistry
- Microbial Metabolites in Food Biotechnology
- Natural Antidiabetic Agents Studies
- Synthesis and Characterization of Heterocyclic Compounds
- RNA Interference and Gene Delivery
- Supramolecular Chemistry and Complexes
- Photosynthetic Processes and Mechanisms
Tokyo Institute of Technology
2015-2024
Institute of Science Tokyo
2024
Osaka Metropolitan University
2023
Tokyo Metropolitan University
2023
Research Institute for Bioscience and Biotechnology
2015
National Cancer Center Hospital East
2013
Keio University
2001-2009
University of Alberta
1992-2003
Mitsubishi Group (Japan)
2002-2003
Mito Saiseikai General Hospital
1999
Hyperfine coupling of a radical ion pair in metal free organic crystals is responsible for room temperature, second-persisting phosphorescence.
Abstract Non-invasive remote control technologies designed to manipulate neural functions have been long-awaited for the comprehensive and quantitative understanding of neuronal network in brain as well therapy neurological disorders. Recently, it has become possible activity be optically manipulated using biological photo-reactive molecules such channelrhodopsin (ChR)-2. However, ChR2 its relatives are mostly reactive visible light, which does not effectively penetrate through tissues. In...
The ring flip of a carbohydrate is employed for the tongs-like movable component metal ion sensor. A pair separated pyrene groups attached to are placed side by when it recognizes ions, affording excimer fluorescence. This novel molecular sensor selective Zn2+ and Cd2+.
Abstract Room‐temperature phosphorescence (RTP) of crystalline isophthalic acid (IPA) through hyperfine‐coupling‐driven (HFC‐driven) intersystem crossing in charge‐transfer (CT) complexes has been discovered recently, giving rise to a new design method for materials and photosensitizers. In this study, we found that phenylboronic (PB) derivatives also demonstrated RTP, as recorded IPA. Magnetic‐field spin‐isotope effects on the emission strength intensities PBs were typical HFC‐driven...
Abstract Reports on the room temperature phosphorescence of metal-free organic crystals have been surging in past few years. Together with interests rare phenomenon, these compounds attracted attention for such potential applications as bio-imaging probes, oxygen sensors, and light-emitting diodes. For common compounds, is emission from a triplet excited state, which usually produced singlet state through intersystem crossing, forbidden spin-flip an electron. The mechanism process key to...
Optogenetics requires implantation of light-delivering optical fibers, as current light-sensitive opsins are activated by visible light, which cannot effectively penetrate biological tissues. Insertion fibers and subsequent photostimulation inherently damages brain tissue, fiber tethering can restrict animal behavior. To overcome these technical limitations, we developed minimally invasive "fiberless" optogenetics using lanthanide micro-particles (LMPs), emit up-conversion luminescence in...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemical-enzymic synthesis of 5'-thio-N-acetyllactosamine: the first disaccharide with sulfur in ring non-reducing sugarHideya Yuasa, Ole Hindsgaul, and Monica M. PalcicCite this: J. Am. Chem. Soc. 1992, 114, 14, 5891–5892Publication Date (Print):July 1, 1992Publication History Published online1 May 2002Published inissue 1 July 1992https://pubs.acs.org/doi/10.1021/ja00040a080https://doi.org/10.1021/ja00040a080research-articleACS PublicationsRequest...
A triplex-forming oligonucleotide (TFO) could be a useful molecular tool for gene therapy and specific modification. However, unmodified TFOs have two serious drawbacks: low binding affinities high sequence-dependencies. In this paper, we propose new strategy that uses set of modified nucleobases four-base recognition TFOs, thereby overcome these drawbacks. containing 2'-deoxy-4N-(2-guanidoethyl)-5-methylcytidine (dgC) residue C-G base pair higher abilities than those...
Familial central diabetes insipidus is an autosomal dominant disease caused by a deficiency of arginine vasopressin (AVP). We previously reported three distinct mutations in the AVP gene Japanese familial pedigrees that result substitution Ser for Gly57 neurophysin-II (NPII) moiety precursor, Thr Ala at COOH-terminus signal peptide, and deletion Glu47 NPII moiety. In this study, we analyzed two direct sequencing polymerase chain reaction-amplified DNA found novel exon 2, which encodes part...
The arginine vasopressin (AVP) gene was sequenced in a pedigree with familial central diabetes insipidus (DI). When polymerase chain reaction-amplified DNAs from affected subjects were subjected to polyacrylamide gel electrophoresis, fragments including exon 2 displayed two additional, slower migrating bands. These extra bands represented DNA heteroduplexes, indicating that there deletion or insertion mutation 2. As the region such identified by direct sequence analysis, subcloned and...
ABSTRACT 5-Thio-L-fucose was synthesized for the first time from D-glucose in 16 steps and -found to have a remarkable inhibitory effect on fucosidases bovine epididymis (Ki 4.2x10−5 M) kidney 8.4 x 10−5 M).
The photosynthetic membranes of cyanobacteria and chloroplasts higher plants have remarkably similar lipid compositions. In particular, thylakoid both are composed galactolipids, which monogalactosyldiacylglycerol (MGDG) is the most abundant, although MGDG biosynthetic pathways different in these organisms. Comprehensive phylogenetic analysis revealed that synthase (MGD) homologs filamentous anoxygenic phototrophs Chloroflexi a close relationship with MGDs Viridiplantae (green algae land...
Monogalactosyldiacylglycerol (MGDG), the most abundant lipid in thylakoid membranes, is involved photosynthesis and chloroplast development. MGDG lipase has an important role remodeling Chlamydomonas reinhardtii. However, process related to turnover of lysogalactolipid that results from degradation, monogalactosylmonoacylglycerol (MGMG), remains be clarified. Here we identified a homolog Arabidopsis thaliana lysophosphatidylcholine acyltransferase (LPCAT) characterized two independent...
Uridine 5′-(5a-carba-α-D-galactopyranosyl diphosphate), the carbocyclic analog of UDP-galactose where ring oxygen galactose residue is replaced by a methylene group, was chemically synthesized as potential inhibitor galactosyltransferases. It found to be competitive UDP-galactose: N-acetylglucosamine β(1 → 4)-galactosyltransferase from bovine milk (EC 2.4.1.22) with K i value 58 µM, similar m for (25 µM). Keywords: carbocycles, UDP-5a-carbagalactose, galactosyltransferase,...
Carbohydrates are among the potential materials for molecular devices, since they abundant natural resources. However, their rigidity has restricted use movable devices. Hinge sugars, 2,4-diamino-2,4-dideoxy-xylopyranosides, shed light on of carbohydrates as components, demonstrated by motion which all four equatorial substituents can change to an axial orientation in synchronization with a chelation-driven 4C1–1C4 ring flip. In this study, we synthesized tong-like metal ion sensor,...
Abstract 5-Thio-D-mannose (1), the first 5-thiosugar found recently in nature, was synthesized 12 steps from D-mannose.
Sialyltransferases (STs) are involved in the biosynthesis of glycoconjugates with important biological activities. Most STs utilize cytidine-5'-monophospho-N-acetylneuraminic acid (CMP−Neu5Ac) as a common donor substrate. A bisubstrate analogue containing substrate (CMP−Neu5Ac mimic) and acceptor (galactose) was synthesized. Four analogues having partial structure were also synthesized to support study structure−activity relationship. Each contains an ethylene group place exocyclic anomeric...
Monogalactosyldiacylglycerol (MGDG), which is conserved in almost all photosynthetic organisms, the most abundant natural polar lipid on Earth. In plants, MGDG highly accumulated chloroplast membranes and an important bulk constituent of thylakoid membranes. However, precise functions photosynthesis have not been well understood. Here, we report a novel synthase from green sulfur bacterium Chlorobaculum tepidum. This enzyme, MgdA, catalyzes synthesis using UDP-Gal as substrate. The gene...
Prevalent photosensitizing agents for photodynamic therapy (PDT) suffer from their relatively large molecular weights causing photodermatosis. In this regard, low weight pyrene could be an efficient photosensitizer except its extreme hydrophobicity. To tackle the insolubility of pyrene, we synthesized 1-carboxypyren-2-yl C-glucoside 4 by a tethered C-glucosylation and 1-pyrenylmethyl O-glucoside 5 simple O-glucosylation. Compounds showed modest water solubilities 72 47 μg/mL, respectively....
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTBending Trisaccharides by a Chelation-Induced Ring Flip of Hinge-Like Monosaccharide UnitHideya Yuasa and Hironobu HashimotoView Author Information Department Life Science Faculty Bioscience Biotechnology Tokyo Institute Technology Nagatsuta, Midori-ku Yokohama 226-8501, Japan Cite this: J. Am. Chem. Soc. 1999, 121, 21, 5089–5090Publication Date (Web):May 15, 1999Publication History Received25 November 1998Published online15 May 1999Published...