A5053290042- Enzyme Structure and Function
- Allergic Rhinitis and Sensitization
- Protein Structure and Dynamics
- Food Allergy and Anaphylaxis Research
- Enzyme Production and Characterization
- Contact Dermatitis and Allergies
- Amino Acid Enzymes and Metabolism
- Advanced Proteomics Techniques and Applications
- Occupational exposure and asthma
- Biochemical and Molecular Research
- HVDC Systems and Fault Protection
- Cancer therapeutics and mechanisms
- Porphyrin Metabolism and Disorders
- Reservoir Engineering and Simulation Methods
- Metal-Catalyzed Oxygenation Mechanisms
- Scientific Computing and Data Management
- Biopolymer Synthesis and Applications
- Advanced Surface Polishing Techniques
- Physics of Superconductivity and Magnetism
- Microbial bioremediation and biosurfactants
- Microbial Natural Products and Biosynthesis
- Phytase and its Applications
- Trace Elements in Health
- Superconducting Materials and Applications
- S100 Proteins and Annexins
Diamond Light Source
2011-2023
University of York
2012-2019
The second-generation high-temperature superconductor (2G HTS), Re-Ba-Cu-O coated conductors are known for strong mechanical properties and have numerous potential applications in high magnetic field research electrical power systems. It has been noted the community that can be prone to performance degradation on thermal cycling when used epoxy impregnated magnet windings. This tied <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">c</i> -axis peel...
To obtain structural insights into the emergence of biological functions from catalytically promiscuous enzymes, we reconstructed an ancestor distinct, but evolutionarily related, haloalkane dehalogenases (EC 3.8.1.5) and Renilla luciferase 1.13.12.5). This has both hydrolase monooxygenase activities. Its crystal structure solved to 1.39 Å resolution revealed presence a catalytic pentad conserved in dehalogenase descendants molecular oxygen bound between two residues typically stabilizing...
Phytases hydrolyse phytate (myo-inositol hexakisphosphate), the principal form of phosphate stored in plant seeds to produce and lower phosphorylated myo-inositols. They are used extensively feed industry, have been characterised biochemically structurally with a number structures PDB. divided into four distinct families: histidine acid phosphatases (HAP), β-propeller phytases, cysteine purple also split three enzyme classes, 3-, 5- 6-phytases, depending on position first inositol ring be...
Stability is one of the most important characteristics proteins employed as biocatalysts, biotherapeutics, and biomaterials, role computational approaches in modifying protein stability rapidly expanding. We have recently identified stabilizing mutations haloalkane dehalogenase DhaA using phylogenetic analysis but were not able to reproduce effects these force-field calculations. Here we tested four different hypotheses explain molecular basis stabilization structural, biochemical,...
Amylases are probably the best studied glycoside hydrolases and have a huge biotechnological value for industrial processes on starch. Multiple amylases from fungi microbes currently in use. Whereas bacterial well suited many due to their high stability, fungal recognized as safe preferred food industry, although they lack pH tolerance stability of counterparts. Here, we describe three amylases, two which broad spectrum extending 8 higher set applications. These enzymes characteristic GH13...
Group 21 and 5 allergens are homologous house dust mite proteins known as mid-tier allergens. To reveal the biological function of group to understand better allergenicity rDer f allergen, we determined 1.5 Å crystal structure allergen from Dermatophagoides farinae. The protein consists a three helical bundle, similar available structures dimer forms hydrophobic binding pocket one in Der p which indicates that both groups could share function. By performing structure-guided mutagenesis,...
Proteins of the DUF866 superfamily are exclusively found in eukaryotic cells. A member superfamily, C1ORF123, is a human protein open reading frame 123 chromosome 1. The physiological role C1ORF123 yet to be determined. only available structure family shares just 26% sequence similarity and does not contain zinc binding motif. Here, we present crystal recombinant (rC1ORF123). has 2-fold internal symmetry dividing monomeric into two mirrored halves that comprise distinct electrostatic...
Dermatophagoides farinae is one of the major house dust mite (HDM) species that cause allergic diseases. N-terminally His-tagged recombinant Der f 21 (rDer 21), a group allergen, with signal peptide truncated was successfully overexpressed in an Escherichia coli expression system. The purified rDer protein initially crystallized using sitting-drop vapour-diffusion method. Well diffracting crystals were obtained after optimization crystallization conditions hanging-drop method reservoir...
TylP is one of five regulatory proteins involved in the regulation antibiotic (tylosin) production, morphological and physiological differentiation Streptomyces fradiae . Its function similar to those various γ-butyrolactone receptor proteins. In this report, N-terminally His-tagged recombinant protein (rTylP) was overproduced Escherichia coli purified homogeneity. The rTylP crystallized from a reservoir solution comprising 34%( v / ) ethylene glycol 5%( glycerol. crystals diffracted X-rays...
With Diamond now firmly into its third phase of construction, the availability new beamlines is allowing industrial usage to thrive. The scope for research and development greater than ever before, our clients probe their systems interest with resolution or under closer realistic operating conditions. establishment liaison activity at Diamond, along a description key market sectors, was described in previous article [1]. current status an insight some Europe-wide initiatives given here.
Burkholderia pseudomallei is a highly versatile pathogen with ~25% of its genome annotated to encode hypothetical proteins. One such protein, BPSL1038, conserved across seven bacterial genera and 654 spp. Here, we present 1.55 Å resolution crystal structure BPSL1038. The overall folded into modified βαββαβα ferredoxin fold similar known Cas2 nucleases. equivalent catalytic aspartate (D11) pairs are in BPSL1038 although B. has no CRISPR associated system. Functional analysis revealed that...
C1ORF123 is a human hypothetical protein found in open reading frame 123 of chromosome 1. The belongs to the DUF866 family comprising eukaryote-conserved proteins with unknown function. Recent proteomic and bioinformatic analyses identified presence brain, frontal cortex synapses, as well its involvement endocrine function polycystic ovary syndrome (PCOS), indicating importance biological role. In order provide better understanding protein, characterization analysis recombinant (rC1ORF123),...
The importance of industrial use national and international infrastructure projects such as Diamond Light Source is well understood, with the facilities ever mindful impact agenda their funding agencies governments. opened its doors for academic users in January 2007, following a year later. Since that time, more than 40 companies have used Diamond's beamlines support laboratories through proprietary access mode, i.e. paying usage. In addition, many collaborated university partners peer review route.