The diversity of yeasts collected from different sites in Antarctica (Admiralty Bay, King George Island and Port Foster Bay Deception Island) their ability to produce extracellular enzymes mycosporines were studied. Samples during the austral summer season, between November 2006 January 2007, rhizosphere Deschampsia antarctica, ornithogenic (penguin guano) soil, marine lake sediments, water freshwater lakes. A total 89 isolates belonging following genera recovered: Bensingtonia, Candida,...

The diversity of yeasts collected from different sites in Antarctica (Admiralty Bay, King George Island and Port Foster Bay Deception Island) their ability to produce extracellular enzymes mycosporines were studied. Samples during the austral summer season, between November 2006 January 2007, rhizosphere Deschampsia antarctica, ornithogenic (penguin guano) soil, marine lake sediments, water freshwater lakes. A total 89 isolates belonging following genera recovered: Bensingtonia, Candida,...

A novel yeast species, Metschnikowia cerradonensis sp. nov., is described from 12 strains isolated flowers of Ipomoea carnea and beetles the genus Conotelus in Cerrado ecosystem region Jalapão, Tocantins State, Brazil. Analysis sequences rRNA gene cluster suggested that M. closely related to santaceciliae , continentalis an undescribed species represented by strain UWOPS 00-154.1. These mate together but ascospores are very rarely formed, showing they represent distinct biological species....

Understanding the environmental factors that shape microbial communities is crucial, especially in extreme environments, like Antarctica. Two main forces were reported to influence Antarctic soil microbes: birds and plants. Both plants are currently undergoing relatively large changes their distribution abundance due global warming. However, we need clearly understand relationship between plants, microorganisms. We therefore collected rhizosphere bulk soils from six different sampling sites...

Thirteen strains of a new ascospore-forming, methanol-assimilating yeast species were isolated from sap exudates Sclerolobium sp. (carvoeiro) in two forest fragments the state Toncantins, Brazil, and Hymenaea courbaril (guapinol, jatobá) Guanacaste Province, Costa Rica. Analysis sequences D1/D2 large-subunit ribosomal DNA showed that belongs to genus Ogataea (syn. Pichia), it was described as falcaomoraisii. The closest relatives are Candida ortonii C. nemodendra. type culture is...

The Anaerobic Digestion Model No. 1 (ADM1) was employed to simulate methane (CH 4 ) production in an anaerobic reactor (AR), and the associated bench-scale biochemical potential (BMP) assay, having sewage sludge (SWS) from a municipal wastewater treatment plant (WWTP) as feedstock. SWS presented following physical-chemical characteristics: pH (7.4–7.6), alkalinity (2,382 ± 100 mg CaCO3 L −1 ), tCOD (21,903 1,000 TOC (895 TS, TVS, VSS (2.0%, 1.1%, 0.8%, respectively). BMP assay conducted six...

Abstract Astrobiology is a relatively recent scientific field that seeks to understand the origin and dynamics of life in Universe. Several hypotheses have been proposed explain cosmic context throughout human history, but only now, technology has allowed many them be tested. Laboratory experiments able show how chemical elements essential life, such as carbon, nitrogen, oxygen hydrogen combine biologically important compounds. Interestingly, these compounds are ubiquitous. How were combined...

Ethidium Bromide (EtBr) is an organic compound used in molecular biology investigations. EtBr ability of intercalating the DNA molecule makes it a toxic substance. The objective was to evaluate phycoremediation potentials Chlorella vulgaris, Desmodesmus subspicatus and Raphidocelis subcapitata tested separately mixture (Mix) for removal from aqueous medium. Experiments were conducted using initial algae biomass 106 cell/mL, exposed 500 µg/L EtBr. efficiency (µg L-1) after 3 h each treatment...

<title>Abstract</title> To evaluate microbial profiling during the anaerobic digestion (AD) of sewage sludge (SWS) from a municipal Wastewater Treatment Plant (WWTP), Biochemical Methane Potential (BMP) assay operating at 37°C mono-digestion was performed. The results 16S ribosomal RNA (rRNA) gene sequencing, utilizing Illumina MiSeq platform, revealed core bacterial community in solid material, dominated by variations profiles. relative abundance SWS (before AD) as following: Proteobacteria...

Resumo: Observações e experiências em nossa formação docente nos levaram a questionar eficiência da aprendizagem por abordagens essencialmente práticas. Este estudo analisa como é trabalhada relação teoria-prática na dinâmica do Curso de Licenciatura Ciências Biológicas Universidade Federal Minas Gerais as conseqüências dessa abordagem prática pedagógica dos professores nele graduados. As ementas algumas disciplinas foram cruzadas com os dados obtidos meio questionários submetidos...

Chlorella vulgaris is one of the most common and characterized algae genus with several applications, including carbon sequestration, biofuel, food production wastewater treatment. sp. are considered suitable to be used as model organisms in space research due their cultivation flexibility. Many studies have been carried out ensure better conditions for supporting human life on long-term missions deep or planetary surfaces, minimizing need resupplies. Regardless resilience has already...

This study aims to evaluate the viability of utilizing Chlorella vulgaris microalgae cultures loaded onto UERJ Sats CubeSat. The objective is develop a compact equipment capable real-time monitoring (C. vulgaris) culture for potential use in CubSat Payload. calibration involved using seven samples laboratory-grown at different densities ranging from 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">6</sup>...

Lung cancer is the most common cause of deaths in both men and women. Adenocarcinoma represents about 28% NSCLC cases 42% EGFR a member ERBB family tyrosine kinases (TK). mutations are more frequently observed female, non-smokers, East-Asian patients with adenocarcinomas, predict response to TK Inhibitors (TKIs). Sections adenocarcinomas lung, formalin-fixed paraffin-embedded tissues (FFPE), were selected analyze exons 19 21 by DNA extraction for polymerase chain reaction (PCR). Exon was...

Lung adenocarcinoma represents about 42% and 28% of NSCLC in women men. Adenocarcinomas incidence is still rising being the most frequent type diagnosed USA. Both EGFR KRAS gene mutations can contribute to development NSCLC, namely adenocarcinomas. are considered by some authors as mutually exclusive explained fact that KRAS-MAPK pathway one downstream signalling pathways EGFR. cancers with resistant tyrosine kinase inhibitors. Sections lung, formalin-fixed paraffin-embedded tissues...

A prognostic interpretation of preneoplastic lesions would have impact in bronchial carcinoma early diagnosis and through the study Erb-B family receptors as they an important role lung carcinogenesis. The existence drugs tirosine kinase inhibitors (TKis) stressed importance studying gene alterations for selected chemoprevention schemes characterization carcinogenesis. Bronchial were characterized by immunohistochemistry using antibodies LP34 (high weigh molecular cytokeratin), CK7,...

MicroRNAs (miRNAs) are a class of naturally occurring small non-coding RNAs that target protein-coding mRNAs at the post-transcriptional level. Expression profiling has identified miRNA signatures in cancers associate with diagnosis, staging, progression, prognosis, and response to treatment. MiRNAs ideal biomarkers FFPE-tissue because, unlike mRNA, integrity is affected very little by formalin fixation. Previous studies have shown miR-21 overexpression correlated poor prognosis NSCLC...