A5005708387- Congenital heart defects research
- Lymphatic System and Diseases
- Tissue Engineering and Regenerative Medicine
- Cardiac Fibrosis and Remodeling
- Neonatal Respiratory Health Research
- Cerebrospinal fluid and hydrocephalus
- Cardiovascular Health and Disease Prevention
University of Oxford
2020-2025
British Heart Foundation
2020-2022
Macrophages are components of the innate immune system with key roles in tissue inflammation and repair. It is now evident that macrophages also support organogenesis, but few studies have characterized their identity, ontogeny function during heart development. Here, we show distribution prevalence resident subepicardial compartment developing coincides emergence new lymphatics, interact closely nascent lymphatic capillaries. Consequently, global macrophage deficiency led to extensive...
Abstract In adult mice, surgically-induced myocardial infarction (MI) activates the cardiac lymphatics, which undergo sprouting angiogenesis, draining interstitial fluid and trafficking macrophages to mediastinal lymph nodes (MLNs), improving function. Given importance of lymphatics post-MI, we investigated their role across neonatal “regenerative window”. At post-natal day 1 (P1) mice fully regenerate heart following MI, in a macrophage-dependent manner, whereas equivalent injury at P7...
During heart development, epicardial cells residing within the outer layer undergo epithelial-mesenchymal transition (EMT) and migrate into underlying myocardium to support organ growth morphogenesis. Disruption of EMT results in embryonic lethality, yet its regulation is poorly understood. Here, we report mesothelial mouse at ultra-high resolution using scanning electron microscopy combined with immunofluorescence analyses. We identified morphologically active regions that associated key...
Abstract Background In adult mice, myocardial infarction (MI) activates the cardiac lymphatics, which undergo sprouting angiogenesis (lymphangiogenesis) and function to drain interstitial fluid traffic macrophages mediastinal lymph nodes (MLNs). This prevents oedema reduces inflammatory immune cell content improve function. Purpose Given importance of lymphatics in macrophage clearance after injury, we investigated their role across neonatal "regenerative window". At post-natal day 1 (P1)...
Abstract Macrophages are components of the innate immune system with key roles in tissue inflammation and repair. It is now evident that macrophages also support organogenesis, but few studies have characterized their identity, ontogeny function during heart development. Here, we show resident subepicardial compartment developing coincide emergence new lymphatics interact closely nascent lymphatic capillaries. Consequently, global macrophage-deficiency led to extensive vessel disruption...
Abstract During embryonic heart development, epicardial cells residing within the outer layer of undergo epithelial-mesenchymal transition (EMT) and migrate into myocardium to support stimulate organ growth morphogenesis. Disruption EMT results in aberrant formation lethality. Despite being an essential process during regulation is poorly understood. Here we report on surface at subcellular resolution using scanning electron microscopy (SEM). We identified high- low-EMT regions mesothelial...