A5038951429- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Nuclear Structure and Function
- Cellular Mechanics and Interactions
- Additive Manufacturing and 3D Printing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Genomics and Chromatin Dynamics
- Electrospun Nanofibers in Biomedical Applications
- DNA Repair Mechanisms
- Tissue Engineering and Regenerative Medicine
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Bone and Dental Protein Studies
- Lymphatic System and Diseases
- Cancer Cells and Metastasis
- Dental materials and restorations
- Skin and Cellular Biology Research
- Angiogenesis and VEGF in Cancer
- Calcium Carbonate Crystallization and Inhibition
- Long-Term Effects of COVID-19
- Lipid Membrane Structure and Behavior
- Periodontal Regeneration and Treatments
- Dental Research and COVID-19
- Cancer Genomics and Diagnostics
- COVID-19 and healthcare impacts
University of Portland
2017-2025
Oregon Health & Science University
2017-2024
OHSU Knight Cancer Institute
2023-2024
University of Pennsylvania
2014-2018
Hebrew University of Jerusalem
2017
Cell migration through solid tissue often involves large contortions of the nucleus, but biological significance is largely unclear. The nucleoskeletal protein lamin-A varies both within and between cell types was shown here to contribute sorting survival in constraining micropores. Lamin-A proved rate-limiting 3D diverse human cells that ranged from glioma adenocarcinoma lines primary mesenchymal stem (MSCs). Stoichiometry A- B-type lamins established an activation barrier, with high...
Cell migration through solid tissue often involves large contortions of the nucleus, but biological significance is largely unclear. The nucleoskeletal protein lamin-A varies both within and between cell types was shown here to contribute sorting survival in constraining micropores. Lamin-A proved rate-limiting 3D-migration diverse human cells that ranged from glioma adenocarcinoma lines primary mesenchymal stem (MSCs). Stoichiometry A- B-type lamins established an activation barrier, with...
Recent studies in tissue engineering have adopted extracellular matrix (ECM) derived scaffolds as natural and cytocompatible microenvironments for regeneration. The dentin matrix, specifically, has been shown to be associated with a host of soluble insoluble signaling molecules that can promote odontogenesis. Here, we developed novel bioink, blending printable alginate (3% w/v) hydrogels the fractions matrix. We optimized printing parameters concentrations individual components bioink print...
Abstract Bone tissue, by definition, is an organic–inorganic nanocomposite, where metabolically active cells are embedded within a matrix that heavily calcified on the nanoscale. Currently, there no strategies replicate these definitive characteristics of bone tissue. Here we describe biomimetic approach supersaturated calcium and phosphate medium used in combination with non-collagenous protein analog to direct deposition nanoscale apatite, both intra- extrafibrillar spaces collagen...
The requirement for immediate vascularization of engineered dental pulp poses a major hurdle towards successful implementation regeneration as an effective therapeutic strategy root canal therapy, especially in adult teeth. Here, we demonstrate novel to engineer pre-vascularized, cell-laden hydrogel pulp-like tissue constructs full-length canals regeneration. We utilized gelatin methacryloyl (GelMA) hydrogels with tunable physical and mechanical properties determine the microenvironmental...
Matrix stiffness that is sensed by a cell or measured purely physical probe reflects the intrinsic elasticity of matrix and also how thick thin is. Here, mesenchymal stem cells (MSCs) their nuclei spread in response to thickness-corrected microelasticity, with increases nuclear tension resulting from myosin-II lamin-A,C. Linearity between widely varying projected area its nucleus across many matrices, timescales, activity levels indicates constant ratio nucleus-to-cell volume, despite MSCs'...
Abstract Biomaterial scaffolds have served as the foundation of tissue engineering and regenerative medicine. However, scaffold systems are often difficult to scale in size or shape order fit defect‐specific dimensions, thus provide only limited spatiotemporal control therapeutic delivery host responses. Here, a lithography‐based 3D printing strategy is used fabricate novel miniaturized modular microcage system, which can be assembled scaled manually with ease. Scalability based on an...
Successful integration of cell-laden tissue constructs with host vasculature depends on the presence functional capillaries to provide oxygen and nutrients embedded cells. However, diffusion limitations biomaterials challenge regeneration large defects that require bulk-delivery hydrogels Herein, a strategy bioprint geometrically controlled, endothelial stem-cell laden microgels in high-throughput is introduced, allowing these cells form mature pericyte-supported vascular vitro, then...
Abstract Bone autografts remain the gold standard for bone grafting surgeries despite having increased donor site morbidity and limited availability. morphogenetic protein‐loaded grafts represent another successful commercial alternative. However, therapeutic use of recombinant growth factors has been associated with significant adverse clinical outcomes. This highlights need to develop biomaterials that closely approximate structure composition autografts, which are inherently...
A hallmark of chronic and inflammatory diseases is the formation a fibrotic stiff extracellular matrix (ECM), typically associated with abnormal, leaky microvascular capillaries. Mechanisms explaining how microvasculature responds to ECM alterations remain unknown. Here, we used microphysiological model capillaries on chip mimicking characteristics healthy or collagen test hypothesis that perivascular cells mediate response vascular mechanical structural changes in human ECM. Capillaries...
The success of tissue engineering inevitably depends on the fabrication constructs that can be vascularized and anastomose with host vasculature. In this report, we studied effects light-emitting diode (LED) photopolymerized gelatin methacryloyl hydrogels (GelMA), encapsulated stem cells from apical papilla (SCAP) human umbilical vein endothelial (HUVECs), in promoting vasculature network formation as a function hydrogel physical mechanical properties, well total cell density. Lithium...
It has long been proposed that recapitulating the extracellular matrix (ECM) of native human tissues in laboratory may enhance regenerative capacity engineered scaffoldsin-vivo. Organ- and tissue-derived decellularized ECM biomaterials have widely used for tissue repair, especially due to their intrinsic biochemical cues can facilitate repair regeneration. The main purpose this study was synthesize a new photocrosslinkable bone-derived hydrogel bioprinting vascularized scaffolds. To end, we...
Abstract Engineered tissue constructs require the fabrication of highly perfusable and mature vascular networks for effective repair regeneration. In engineering, stem cells are widely employed to create vascularized tissues in vitro. Pericytes key maturity these networks, therefore ability differentiate into pericyte-like lineages should be understood. To date, there is limited information regarding from different sources pericytes form microvascular capillaries Therefore, here we tested...
Adequate vascularization of scaffolds is a prerequisite for successful repair and regeneration lost damaged tissues. It has been suggested that the maturity engineered vascular capillaries, which largely determined by presence functional perivascular mural cells (or pericytes), plays vital role in maintaining vessel integrity during tissue regeneration. Here, we investigated pericyte-supported-engineered capillaries regenerating bone critical-size rat calvarial defect model. Prior to...
Abstract Biomimetically mineralized collagen scaffolds are promising for bone regeneration, but vascularization of these materials remains to be addressed. Here, we engineered using an osteopontin‐guided polymer‐induced liquid‐precursor mineralization method recapitulate bone's nanostructure. SEM images samples confirmed the presence with intrafibrillar mineral, also EDS spectra and FTIR showed high peaks calcium phosphate, a similar mineral/matrix ratio native bone. Mineralization increased...