In cancer plasmonic photothermal therapy (PPTT), nanoparticles are used to convert light into localized heat, leading cell death. Among nanoparticles, gold nanorods (AuNRs) with specific dimensions enabling them absorb near-infrared laser have been widely used. The detailed mechanism of PPTT therapy, however, still remains poorly understood. Typically, surface-enhanced Raman spectroscopy (SERS) has detect time-dependent changes in the intensity vibration frequencies molecules that appear or...

Prostate cancer (PCa) is the second leading cause of cancer-related mortality in men. The prevalent diagnosis method based on serum prostate-specific antigen (PSA) screening test, which suffers from low specificity, overdiagnosis, and overtreatment. In this work, untargeted metabolomic profiling age-matched samples prostate patients healthy individuals was performed using ultraperformance liquid chromatography coupled to high-resolution tandem mass spectrometry (UPLC-MS/MS) machine learning...

Non-alcoholic fatty liver disease (NAFLD) is the most common chronic in parts of world. Although there no first-line drug approved for treatment NAFLD, polyene phosphatidylcholine (PPC) used by clinicians to treat NAFLD patients. This study aimed evaluate efficacy PPC on a mice model and PPC's mechanism action. The were fed choline-deficient, L-amino acid-defined (CDAA) diet induce subsequently treated with PPC. effects evaluated index, histopathological examination, routine blood chemistry...

Lung cancer is one of the most common malignancy in world, and early detection lung remains a challenge. The exhaled breath condensate (EBC) from trachea can be collected totally noninvasively. In this study, our aim to identify differential metabolites between non-small cell (NSCLC) control EBC samples discriminate NSCLC group by orthogonal projections latent structures-discriminant analysis (OPLS-DA) models. patients (n = 29) controls 24) (20 healthy 4 benign individuals) were identified...

Anti-PD-1 and anti-PD-L1 antibodies have achieved great clinical success in cancer immunotherapy, peptide small molecule inhibitors of PD-1/PD-L1 binding also attract much attention. Ascidians are not only seafood, but an important source bioactive substances, including anti-tumor components. In this study, ascidian enzymatic hydrolysates were found to contain inhibitory Affinity ultrafiltration (AUF) coupled with the nanoLC-MS/MS method was first applied screening for PD-L1 from...

Progressive lung function decline and, ultimately, respiratory failure are the most common cause of death in patients with cystic fibrosis (CF). This is punctuated by acute pulmonary exacerbations (APEs), and many cases, there a to return baseline function. Ultraperformance liquid chromatography quadrupole-time-of-flight mass spectrometry was used profile metabolites exhaled breath condensate (EBC) samples from 17 clinically stable CF patients, 9 an APE severe enough require hospitalization...

Flow injection-traveling-wave ion mobility-mass spectrometry (FI-TWIM-MS) was applied to the nontargeted metabolic profiling of serum extracts from 61 prostate-cancer (PCa) patients and 42 controls with an analysis speed 6 min per sample, including a 3 wash run. Comprehensive data mining domain used discriminate species various charge states filter matrix salt-cluster ions. Specific criteria were developed ensure correct grouping adducts, in-source fragments, impurities in set. Endogenous...

The most common cause of death in cystic fibrosis (CF) patients is progressive lung function decline, which punctuated by acute pulmonary exacerbations (APEs). A major challenge to discover biomarkers for detecting an oncoming APE and allow pre-emptive clinical interventions. Metabolic profiling exhaled breath condensate (EBC) samples collected from CF before, during, after APEs under stable conditions (n = 210) was performed using ultraperformance liquid chromatography (UPLC) coupled...

Abnormal tryptophan metabolism is linked to cancer and neurodegenerative diseases, metabolites have been reported as potential prostate (PCa) biomarkers. However, little known about the bioactivities of on PCa cell growth. In this study, MTT transwell assays were used study cytotoxicities 13 major normal epithelial lines. Ultraperformance liquid chromatography–high resolution mass spectrometry (UPLC–HRMS) was analyze metabolic changes in cells treated with tryptamine. Flow cytometry,...

Cystic fibrosis (CF) is an autosomal recessive disorder caused by mutations in the gene that encodes cystic transmembrane conductance regulator (CFTR) protein. The vast majority of mortality due to progressive lung disease. Targeted and untargeted CF breath metabolomics investigations via exhaled condensate (EBC) analyses have potential expose metabolic alterations associated with pathology aid assessing effectiveness therapies. Here, transmission-mode direct analysis real time traveling...

Abstract Objective Quantitative Structure-Activity Relationship (QSAR) models can predict adverse drug reactions (ADRs), and thus provide early warnings of potential hazards. Timely identification safety concerns could protect patients aid diagnosis ADRs among the exposed. Our objective was to determine whether global spontaneous reporting patterns might allow chemical substructures associated with Stevens-Johnson Syndrome (SJS) be identified utilized for ADR prediction by QSAR models....

Non-small cell lung cancer (NSCLC) is the prevalent histological subtype of cancer. In this study, we performed ultraperformance liquid chromatography-high-resolution mass spectrometry (UPLC-HRMS)-based metabolic profiling 227 tissue samples from 79 patients with adenocarcinoma (AC) or squamous carcinoma (SCC). Orthogonal partial least squares-discriminant analysis (oPLS-DA) analyses showed that AC, SCC, and NSCLC tumors were discriminated adjacent noncancerous (ANT) distant (DNT) good...

Iron deficiency anemia (IDA) is the most common nutrient-related health problem in world. There still a lack of comprehensive comparative study on efficacies commonly used iron supplements such as polysaccharide complex (PIC), protein succinylate (IPS) and ferrous succinate (FS) for IDA. In this study, we compared PIC, IPS FS IDA rats via intragastric administration. The results showed that three had similar efficacies. PIC/IPS/FS at dose 15 mg Fe/kg/d 10 d increased hematological serum...

Thymoma is a rare malignant tumor but it the most common primary of anterior mediastinum. The current imaging methods for thymoma screening suffer from false positive rate problems, and pathogenesis remains elusive. Study metabolic characteristics could provide clues improving diagnosis understanding thymoma.Metabolic profiling plasma thymic hyperplasia patients was performed using ultrahigh-performance liquid chromatography combined with high-resolution mass spectrometry in both negative...

While online advertising is one of the major sources income for search engines, pumping up incomes from business advertisements while ensuring user experience becomes a challenging but emerging area. Designing high-quality with persuasive content has been proved as way to increase revenues through improving Click-Through Rate (CTR). However, it difficult scale design ads, due lack automation in creativity. In this paper, we present Commonsense-Enriched Advertisement on Search Engine (CHASE)...

Nonalcoholic fatty liver disease (NAFLD) is highly prevalent with identifiable risk factors including obesity, hyperlipidemia and diabetes. A lipidomic approach was taken to quantify the major lipid classes in human serum. Comprehensive characterization of serum lipids determined by ultraperformance liquid chromatography (UPLC)-mass spectrometry (MS) performed NAFLD patients healthy controls. Nine subgroups lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE),...

Abnormal lipid metabolism plays an important role in cancer development. In this study, nontargeted lipidomic study on 230 tissue specimens from 79 nonsmall cell lung (NSCLC) patients was conducted using ultraperformance liquid chromatography-high-resolution mass spectrometry (UPLC-HRMS). Downregulation of sphingosine and medium-long-chain ceramides short-medium-chain acylcarnitine, upregulation long-chain acylcarnitine C20:0, enhanced histamine methylation were revealed NSCLC tissues....

Thymoma is a rare thymic tumor but the most common anterior mediastinal tumor. Currently, there are still limitations regarding to its screening and pathology remains elusive. In this study, nontargeted metabolomic analysis of serum was performed study metabolic characteristics thymoma using ultra-performance liquid chromatography—high resolution mass spectrometry (UPLC-HRMS) in positive negative ionization modes. The metabolites that were differentially abundant identified by MS/MS matching...

Abstract The discovery of new anticancer drugs targeting the PD-1/PD-L1 pathway has been research hotspots. In this study, a combination biological affinity ultrafiltration (BAU), UPLC-HRMS, molecular dynamic (MD) simulations and docking methods were applied to search for endogenous active compounds that can inhibit binding PD-L1 PD-1. We screened dozens potential cancer related compounds. results showed cyclic adenosine monophosphate (cAMP) had direct inhibition effect on with an in vitro...