Lipoprotein synthesis by the colonic adenocarcinoma cell line Caco-2 was investigated to assess utility of this as a model for in vitro study human intestinal lipid metabolism. Electron micrographic analysis conditioned medium revealed that under basal conditions culture post-confluent cells synthesize and secrete lipoprotein particles. Lipoproteins density (d) less than 1.063 g/ml consist heterogeneous population particles (diameter from 10 90 nm). This fraction consists very low...

Measurements of upper-air densities and temperatures from 12 flights the rocket-borne falling sphere are presented. They show little seasonal variation at 32° 38° N, but large variations in a few winter days 59°N. A low arctic density 60 km results linear latitude gradient about 2 per cent degree latitude. Such was measured survey. An abrupt warming 45 detected Fort Churchill January 1958 related to larger scope by balloons. The average air above March warmer 75 cooler 50 30 than 38°N.

Replication-deficient adenoviral vectors have been developed for the delivery of DNA sequences encoding a variety proteins intended management disease through gene therapy. One concern is occur- rence replication-competent adenovirus (RCA) in population replication-deficient as result recombination or contamination. To address this concern, it necessary to determine fre- quency occurrence and fully characterize molecular structure biological infectivity RCA. rAd/p53 pIX-deleted p53 therapy...

A quadrupole mass spectrometer with an ion source designed to minimize recombination effects was flown on a Nike-Cajun rocket, NASA 10.91 UA, from Wallops Island (latitude 37°50′N, longitude 75°29′W) at 1302 EST, May 18, 1962, altitude of 134.5 km. The results indicate ratio O/O2 number densities 3.1 apogee rms error estimated 0.3. At apogee, the N/N2 less than 0.05, and currents due constituents greater 40 0.04 total current. mean molecular weight computed data 133 km is 25.5 0.4. These are...

We have analyzed determinants of the synthesis and secretion apolipoproteins including mRNA for apolipoproteins, in human colon carcinoma cell line Caco-2 during differentiation continuous culture. Significant increases both cellular secreted apolipoprotein A-I were observed early process. Increases B limited to protein started later Levels A-I, A-IV, B, C-III, E increased significantly between time cells reached confluence 1 week postconfluence. The kinetics accumulation influenced by...

Apolipoprotein A-I (apoA-I) is the major protein constituent of plasma high density lipoproteins (HDL). To examine apoA-I processing and secretion, human gene (2.2-kilobase PstI-PstI fragment) linked to mouse metallothionein promoter was transfected by electroporation into NIH 3T3 fibroblasts along with plasmid pSV2 neo, which confers neomycin resistance. Transfected cells were selected for resistance screened ability produce enzyme-linked immunosorbent assay. In absence lipids in medium,...

A diurnal pair of upper atmosphere composition payloads was launched near 9°30′ south latitude aboard Nike-Apache rockets on March 11, 1965. Higher O/O2 ratios were obtained in the daytime than at night, and values approximately equal to those Wallops Island. The data indicated existence atomic nitrogen order 1 2% molecular species, but extent possible instrumental observational errors precludes a firm conclusion this respect. Argon-nitrogen turbopause levels 101 103 km deduced from...

Abstract In manufacturing monoclonal antibodies (mAbs), it is crucial to be able predict how process conditions and supplements affect productivity quality attributes, especially glycosylation. Supplemental inputs, such as amino acids trace metals in the media, are reported cell metabolism glycosylation; quantifying their effects essential for effective development. We aim present validate, through a commercially relevant culture process, technique modeling efficiently. While existing models...

The Manufacturing Readiness Levels (MRLs) developed by the Department of Defense are well-established tools for describing maturity new technologies resulting from government-sponsored Research and Development programs, concept phase to commercial deployment. While MRLs generally applicable a wide range industries technologies, there is significant value in offering an industry-specific view on how basic principles may be applied biomanufacturing. This paper describes Biomanufacturing (BRLs)...

Abstract The move to integrated continuous bioprocessing (ICB), while providing a means for process intensification, can put added strain on analytics when conventional methods are used. For instance, traditional microbial provide minimal value ICB processes given that the time required data become available is much longer than typical full cycle of manufacturing process. Although rapid detection has been in discussion over 30 years, it still not routinely deployed commercial...

The ambr bioreactors are single‐use microbioreactors for cell line development and process optimization. With operating conditions large‐scale biopharmaceutical production properly scaled down, such as the ambr15™ can potentially be used to predict effect of changes modified media or different lines. While there have been some recent studies evaluating technology a scale‐down model fed‐batch operations, little has reported semi‐continuous continuous operation. Gassing rates dilution in were...

High throughput (HT) platforms serve as a cost-efficient and rapid screening method for evaluating the effect of cell-culture conditions chemicals. We report development HT cell-based microarray platform to assess culture on Chinese hamster ovary (CHO) cells. Specifically, growth, transgene expression metabolism GS/methionine sulphoximine (MSX) CHO cell line, which produces therapeutic monoclonal antibody, was examined using system in conjunction with conventional shake flask non-proprietary...

Abstract Recombinant human adenovirus (rhAd) has been used extensively for functional protein expression in mammalian cells including those of and nonhuman origin. High‐level production by rhAd vectors is expected their permissive host cells, such as the embryonic kidney 293 (HEK293) cell line. This attributed primarily to permissiveness HEK293 infection ability support viral DNA replication providing missing El proteins. However, tend suffer from cytopathic effect (CPE) a result virus...

The production of maltase, an inducible and repressible catabolic enzyme in Saccharomyces italicus , was studied compared batch, fed-batch, continuous fermentations. Tight genetic controls on maltase synthesis limited the effect environmental manipulations such as fed-batch or culture enhancement synthesis, neither approach able to improve performance above batch process for production. S. mutated, a constitutive producer isolated. mutant detected by its ability grow sucrose, which is...