Trametes versicolor and Flammulina velutipes, after submerged cultivation, with intermittent exposure to ultraviolet light (UV), were studied for Total Phenolic content (TP) Proteins (TPR) contents antioxidant properties against free radicals. The TP TPR determined by a spectrophotometric method Lowry's assay, respectively. Liquid chromatography mass spectrometry (LC-MS/MS) was used quantify polyphenols. Different in vitro assays the activities of extracts. Mycelia extract from F. velutipes...

The in vitro antioxidant and antimicrobial activities of two Achillea millefolium (Adanson) Koch s.l species essential oils (A. collina Becker ex Heimerl s.l. A. pannonica Scheele, Asteraceae) originating from the Golija Radan mountains (Serbia) were investigated. chemical profiles evaluated by GC-MS. Antioxidant activity was assessed as free radical scavenging capacity (RSC) towards 2,2-diphenyl-1-picrylhydrazil (DPPH) radicals, together with effects on lipid peroxidation (LP)....

The aims of study were to examine the antibacterial potential two commercial essential oils (EOs) from coriander (Coriandrum sativum L.) and thyme (Thymus vulgaris against vaginal clinical strains bacteria yeast their chemical composition.Antimicrobial activities determined using macro-diffusion (disc, well) micro-dilution method in 96-well micro plates twelve bacteria: Escherichia coli, Proteus mirabilis, Staphylococcus aureus Enterococcus sp., Staph. ATCC 25923, 6538 E. coli 25922 Candida...

Lymphangiomas are benign vascular malformations of the lymphatic system and most commonly present in neck area. Large lymphangiomas may compress and/or displace larynx, trachea esophagus cause serious respiratory feeding problems neonates. Prenatal therapy could eliminate risks mentioned complications. include EXIT (ex utero intrapartum treatment) procedure. As this procedure has certain for both neonate mother, introduction a safer method is justified. The use OK-432, as sclerosing agent,...

In this study, we tried to determine whether the activities of primary antioxidant enzymes are detectable in amniotic fluid and they can be used as early biomarkers complications pregnancy such pregnancy-induced hypertension (PIH), gestational diabetes mellitus (GDM), bacterial vaginosis.This was a prospective study which taken between 16 19 week gestation. all, 161 pregnant women were divided into two groups: group - patients with treated local infection, PIH, GDM, control healthy women....

This study aimed to investigate the application of Origanum vulgare L. essential oil (EO) as an alternative antimicrobial agent against vaginal infections.Microdilution methods were applied for detection minimal inhibitory (MIC) and bactericidal/fungicidal (MBC/MFC) concentration 15 clinical strains originating from women with symptoms infection. Optical density determination (OD) was used Candida growth rate. Staining DAPI detect influence EO on nuclear condensation fragmentation, while...

St. John's wort (Hypericum perforatum, Hypericaceae) has long been used in traditional medicine as a potent remedy, while many other species of this genus have not thoroughly investigated. The study aimed to detect the biological activity, including antioxidant, antihyperglycemic, anticholinergic, antimicrobial and monoaminoxidase inhibitory potential, water-alcoholic extracts three autochthonous for Serbia Greece from plant Hypericum (section Hypericum-H. tetrapterum, H. maculatum ssp....

Treating inflammatory conditions .such as vaginosis, vaginitis, and vulvovaginitis in pregnancy is- a special problem due to limitations. of available drugs. However, possible treatment options can be found also naturally originated products, such essential oils (EOs) different plants. The aim this study was evaluate -vitro antimicrobial toxic activities the commercial EO Rosmiarinus officinalis L. (Lamiaceae) against five Gram-positive Gram-negative bacterial strains two Candida obtained...

St. John's wort (

Aim: To determine the existence and level of oxidative stress caused by lipid peroxidation in pregnancy. Methods: The research was conducted as prospective examination that included 60 healthy women (age 18–45). were divided into two groups. group I (N-31) first trimester normal, II nongravid (N-29). Concentrations markers malondialdehyde (MDA) thiobarbituric acid (TBARS) determined using commercial ELISA tests OxiSelect™ TBARS Assay Kit MDA Kit. Results: results this indicate concentrations...

Aromatic plants are among the most important sources of biologically active secondary metabolites, with high antimicrobal potential. This study was carried out to examine in vitro antifungal activity Foeniculum vulgare (Apiaceae), Carum carvi (Apiaceae) and Eucalyptus sp.(Myrtaceae) essential oils against three Candida albicans strains different origin (laboratory-CAL, human pulmonary-CAH ATCC10231-CAR). The were screened on C. using disc well-diffusion microdilution method, compared...

Abstract Aim: To evaluate serum level of proinflammatory cytokine interleukin-8 as a biochemical marker local infections in patients with clinical symptoms preterm deliveries. Methods: We studied 74 pregnant women at 24–36 gestational weeks (GW) delivery. Serum was measured. Results: The mean value the study group (n=36) 19.13 pg/mL vs. 5.02 controls (n=38). Conclusions: results indicate that might be used non-invasive pregnancy, well

Abstract Background The aim of this study was to evaluate the markers oxidative stress (OS), as well magnesium (Mg) concentration, pregnancy-associated plasma protein A (PAPP-A) and human chorionic gonadotropin (β-hCG) values in patients with previous recurrent miscarriages, purpose comparing level OS normal pregnancy. Methods conducted a prospective study, which included total 95 pregnant women divided into two groups. In group (n = 35), there were at least three consecutive miscarriages....

Objective. To study the role of asymptomatic maternal parvo B19 infection in severe fetal outcome Province Vojvodina.Methods. One hundred seventy-six pregnant women (13–25 weeks gestation) were divided two groups – patients with symptoms imminent spontaneous abortion and poor pregnancy normal course pregnancy. Double serum samples analyzed to quantify IgM IgG parvovirus B19.Results. Among abortion, we found significantly higher percentage acute infection.Conclusions. Asymptomatic is...

Induction of labor is one the most common obstetric interventions in contemporary obstetrics.The aim study was to evaluate clinical and sonographic parameters prediction success induction.The prospective included 422 women whom induction carried out at Department Obstetrics Gynecology Clinical Centre Vojvodina. The role body mass index age women, parity Bishop score, cervical length measured by transvaginal ultrasound evaluated regard induction, which considered successful if a vaginal...

In this study we tried to determine if the activities of primary antioxidant enzymes are detectable in amniotic fluid and they can be used as early biomarkers complications pregnancy connected with bacterial vaginosis.This was a prospective which taken between 16 19 weeks gestation. 161 pregnant women were divided into two groups: group--patients treated local infection control group--healthy women. Levels reduced glutathione, glutathione peroxidase, reductase, S-transferase, xanthine...

Bacground/Aim. Preterm delivery is one of the most common complications in pregnancy, and it major cause (75- 80%) all neonatal deaths. Bacterial vaginosis predisposes to an increased risk preterm delivery, premature rupture membrane miscarriage. In this syndrome normal vaginal lactobacilli, which produce protective H2O2, are reduced replaced with anaerobic, gram-negative bacteria others. The aim study was evaluate influence bacterial on week biochemical markers inflammation serum. Methods....

Introduction. Serbia is the country with extremely low birth rate and a relatively high percentage of preterm deliveries (8%). With this in mind, discovering new diagnostic methods that could be used for prediction delivery great importance. In study we tried to determine whether bacterial vaginosis chlamydial infection provoke by activation systemic cytokine network. Objective. The aim was serum levels proinflammatory cytokines (IL-1?, IL-8, IFN-?, IL-6 TNF-?) pregnant women symptoms make...

To determine if there is any difference in amniotic fluid erythropoietin (EPO) concentration between fetuses small for gestational age (SGA) and appropriate (AGA), the constitutionally (CSF) growth-restricted (GRF) fetuses.EPO concentrations samples were determined by EpoELISA test 38 pregnancies with SGA 15 AGA fetuses. In group we measured Ponderal index (PI) skin-fold thickness (SFT). If PI and/or SFT below 10th percentile neonate was GRF. both above CSF.Higher levels of EPO detected...

Normal placental vascular development depends on multiple interactions of many regulatory molecules including pro and antiangiogenic proteins. It is considered that these modulators might be one the factors responsible for hypertensive disorders in pregnancy.To evaluate compare early pregnancy (11-14 week gestation) serum level angiogenic proteins sFlt1, VEGF i PIGF between different types related disorders.The study included 177 pregnant women 11 14 weeks gestation, divided into four...

Objectives: To find out if determination of IL-4, IL-13 and IL-6 in amniotic fluid (AF) serum mid-pregnancy can be used as predictors preterm delivery.Material methods: The research has comprised 88 pregnant women at 16-24 weeks gestation, who were subjected to early amniocentesis. Levels interleukins determined by ELISA tests. Pregnant had been monitored untill termination pregnancy divided two groups: 68 term 20 deliveries.Results: was not detectable all samples. IL-4 samples both groups...

Introduction. Preterm delivery (PTD), defined as a between 24th and 37th completed week, increases the risk of neonatal morbidity mortality. There is growing body evidence that intrauterine infection well cervicovaginal bacterial infections Chlamydia are possible causes preterm delivery. Host response to and/or coupled with release various inflammatory mediators, many which cytokines. Objective. The aim study was find out if cervical influenced serum levels interferon-? (IFN-?), interleukin...

Background/Aim. Preeclampsia (PE) is a multisystemic syndrome that complicates 5?8% of all pregnancies. The aim this study was to evaluate the biochemical parameters oxidative stress in first trimester pregnancy patients with preeclampsia, purpose comparing level normal pregnancy. Methods. conducted as prospective study. It included totally 107 pregnant women divided into two groups. In group (n = 33) there were who developed preeclampsia current control 74) healthy women. Blood samples...

Background and Objectives: Monitoring pregnancies with fetal growth restriction (FGR) presents a challenge, especially concerning the time of delivery in cases early preterm below 32 weeks. The aim our study was to compare different diagnostic parameters growth-restricted neonates without morbidity/mortality determine sensitivity specificity for monitoring Materials Methods: Our clinical evaluated 120 deliveries, gestational age ≤ + 0 weeks, prenatally diagnosed placental FGR. All patients...

Introduction. Antibiotic prophylaxis means administration of antibiotics in prevention infections. Objective. To investigate the efficacy a single dose preoperative ceftriaxone and cefazolin intra- postoperative infections parturients without high risk inflammation. Methods. The first group 45 pregnant 4 non-pregnant women were preoperatively administered 2 g, i.v., 10 minutes before planned surgery. second g concentrations estimated immediately 6 hours following operation, as well amniotic...