A5061767601- Autophagy in Disease and Therapy
- Advanced Glycation End Products research
- Pancreatic function and diabetes
- Kruppel-like factors research
- Microtubule and mitosis dynamics
- Diabetes and associated disorders
- Cancer-related Molecular Pathways
- Cancer, Stress, Anesthesia, and Immune Response
- Genomics, phytochemicals, and oxidative stress
- Gastrointestinal motility and disorders
- Diet, Metabolism, and Disease
- Helicobacter pylori-related gastroenterology studies
- Sirtuins and Resveratrol in Medicine
- Cancer Treatment and Pharmacology
- Cell death mechanisms and regulation
- Cannabis and Cannabinoid Research
- Polysaccharides and Plant Cell Walls
Chung-Ang University
2019-2022
Paclitaxel is an anti-microtubule agent that has been shown to induce cell death in gastric cancer. However, the detailed mechanism of action unclear. In this study, we reveal paclitaxel-induced involves mitotic catastrophe, autophagy and apoptosis AGS cells. induced intrinsic by activating caspase-3, caspase-9 PARP. addition, significant increase marker LC3B-II, together with Atg5, class III PI3K Beclin-1, down-regulation p62 following paclitaxel treatment verified autophagy. Further...
Background/Aim: Fluoxetine, an antidepressant, has cytotoxic effects on several cancer cell lines, while paclitaxel is antineoplastic agent for various cancers. The aim of this study was to evaluate whether fluoxetine enhances the effect in gastric adenocarcinoma cells and determine mechanism death. Materials Methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay used examine viability perform cycle analysis. Annexin V propidium iodide (PI) staining,...
Fluoxetine is used widely as an antidepressant for the treatment of cancer-related depression, but has been reported to also have anti-cancer activity. In this study, we investigated cytotoxicity fluoxetine human gastric adenocarcinoma cells; shown by MTT assay, induced cell death. Subsequently, cells were treated with 10 or 20 µM 24 h and analyzed. Apoptosis was confirmed increased number early apoptotic cells, Annexin V- propidium iodide staining. Nuclear condensation visualized DAPI A...
Gastric adenocarcinoma is among the top causes of cancer-related death and one most commonly diagnosed carcinomas worldwide. Benzyl isothiocyanate (BITC) has been reported to inhibit gastric cancer metastasis. In our previous study, BITC induced apoptosis in AGS cells. The purpose present study was investigate effect on autophagy mechanism First, cells were treated with 5, 10, or 15 μM for 24 h, followed by an analysis mechanism. expression level proteins involved different steps autophagy,...
Chronic stress can cause the gastrointestinal disorders characterized by an altered bowel movement and abdominal pain. Studies have shown that Humulus japonicus extract (HJE) has anti-inflammatory antidiarrheal effects, Phragmites rhizoma (PEP) antioxidative antistress effects. The present study aimed to investigate possible effects of HJE PEP in rat models with stress-induced dysfunctions. rats were exposed water avoidance (WAS, 1 h/day) for 10 days induce disorders. We found WAS...
Abstract Paclitaxel is an anti-microtubule agent that has been shown to induce cell death in gastric cancer. However, the detailed mechanism of action unclear. In this study, we reveal paclitaxel-induced involves mitotic catastrophe, autophagy and apoptosis AGS cells. induced intrinsic by activating caspase-3, caspase-9 PARP. addition, significant increase marker LC3B-II, together with Atg5, class III PI3K Beclin-1, down-regulation p62 following paclitaxel treatment verified autophagy....