A5028022652- Muscle Physiology and Disorders
- Cardiomyopathy and Myosin Studies
- Muscle activation and electromyography studies
- Adipose Tissue and Metabolism
- Cardiovascular Effects of Exercise
- Bone health and osteoporosis research
- Genetics and Physical Performance
- Ion channel regulation and function
- Muscle metabolism and nutrition
- Exercise and Physiological Responses
- Clinical practice guidelines implementation
- Bone Metabolism and Diseases
- Vitamin D Research Studies
- Tea Polyphenols and Effects
- Cardiovascular and exercise physiology
- Phytochemicals and Antioxidant Activities
- Heavy Metals in Plants
- Bone and Dental Protein Studies
- Body Composition Measurement Techniques
- Nutrition and Health in Aging
- Infant Nutrition and Health
- Spaceflight effects on biology
- Birth, Development, and Health
- Diet and metabolism studies
- Hip and Femur Fractures
Brock University
2014-2024
Osteoporosis Canada
2023
In Canada, more than 2 million people live with osteoporosis, a disease that increases the risk for fractures, which result in excess mortality and morbidity, decreased quality of life loss autonomy. This guideline update is intended to assist Canadian health care professionals delivery optimize skeletal prevent fractures postmenopausal females males aged 50 years older.This an 2010 Osteoporosis Canada clinical practice on diagnosis management osteoporosis Canada. We followed Grading...
Globally, traditional and herbal teas are a prominent dietary source of polyphenols, represent class bioactive molecules that closely associated with variety health benefits. Most consumers prepare tea using bags, although there is little information about whether this production step alters the content final product. The study purpose was to investigate effect steep time type on polyphenol predicted antioxidant capacity commercially available bag products, including Green, Orange Pekoe, Red...
The increase in isometric twitch force observed fast-twitch rodent muscles during or after activity, known universally as potentiation, is normally associated with myosin regulatory light chain (RLC) phosphorylation. Interestingly, fast from mice devoid of detectable skeletal kinase (skMLCK) retain a reduced ability to potentiate force, indicating the presence secondary origin for this characteristic feature muscle phenotype. purpose study was assess changes intracellular cytosolic free Ca2+...
Pyruvate dehydrogenase (PDH) plays an important role in regulating carbohydrate oxidation skeletal muscle. PDH is deactivated by a set of kinases (PDK1, PDK2, PDK3, PDK4), with PDK2 and PDK4 being the most predominant isoforms Although abundant isoform, few studies have examined its physiological role. The on activation (PDHa) at rest during muscle stimulation 10 40 Hz (eliciting low- moderate-intensity contractions, respectively) isolated extensor digitorum longus muscles was studied...
We examined the effects of ∼30 days spaceflight on glycogen synthase kinase 3 (GSK3) content and inhibitory serine phosphorylation in murine muscle bone samples from four separate missions (BION-M1, rodent research [RR]1, RR9, RR18). Spaceflight reduced GSK3β across all missions, whereas its was elevated with RR18 BION-M1. The reduction linked to type IIA fibers commonly observed as these are particularly enriched GSK3. then tested inhibiting GSK3 before this fiber shift, we demonstrate that...
The purpose of this study was to test the hypothesis that potentiation concentric twitch force during work cycles is dependent upon both speed and direction length change. Concentric eccentric forces were elicited by stimulating muscles shortening lengthening phases, respectively, cycles. Work cycle frequency varied in order vary muscle and/or lengthening; all measured as passed though optimal (L(o)). Both assessed before (unpotentiated control) after (potentiated) application a tetanic...
Scope The effects of a long‐term high fat and sucrose diet (HFS) superimposed with aging on bone muscle structure and/or function. Methods results Male C57BL/6J mice (20 weeks age) were randomized to 1 3 groups: baseline (BSL, n = 12), or assigned control (AGE, 12) HFS (HFS‐AGE, 11) for 13 weeks. Trabecular structure, volumetric mineral density (vBMD), body composition, measured longitudinally at 20, 24, 32 age. In vitro contractile measures performed isolated soleus extensor digitorum...
Posttetanic potentiation (PTP) and the catchlike property (CLP) enhance contractile function in skeletal muscle. We investigated CLP during dynamic performance mouse hindlimb muscles with (wild-type) without (skMLCK(-/-) ) primary mechanism for PTP (myosin phosphorylation) (in vitro, 25°C).Extensor digitorum longus of both genotypes were stimulated constant frequency trains (CFT CLT), before after a potentiating stimulus (PS).Before PS, CLT increased concentric force/work relative to CFT,...
The isometric potentiation associated with myosin phosphorylation is force dependent. purpose of this study was to assess the influence a pre-existing period on concentric displayed by mouse muscles and without ability phosphorylate myosin. We tested (ISO) (CON) potentiation, as well after (ISO-CON), in from wild-type (WT) skeletal light chain kinase-deficient (skMLCK −/− ) mice. A conditioning stimulus increased (i.e., potentiated) mean ISO-CON CON conditions 1.31 ± 0.02 1.35 1.19 1.21 0.01...
Skeletal myosin light chain kinase (skMLCK) catalyzed phosphorylation of the regulatory (RLC) increases (i.e. potentiates) mechanical work output fast skeletal muscle. The influence this event on contractile economy energy cost/work performed) remains controversial, however. Our purpose was to quantify potentiated extensor digitorum longus (EDL) muscles from mouse with (wildtype, WT) and without (skMLCK ablated, skMLCK−/−) ability phosphorylate RLC. Contractile calculated as ratio total...
Phosphorylation of the myosin regulatory light chain (RLC) by skeletal kinase (skMLCK) potentiates rodent fast twitch muscle but is an ATP-requiring process. Our objective was to investigate effect skMLCK-catalyzed RLC phosphorylation on energetic cost contraction and contractile economy (ratio mechanical output metabolic input) mouse in vitro (25°C). To this end, extensor digitorum longus (EDL) muscles from wild-type (WT) skMLCK-devoid (skMLCK-/-) mice were subjected repetitive...
Most studies show a positive association between higher tea intake and bone mineral density (BMD), while associations with fracture are inconclusive. Although few have investigated the potential effects of rooibos on cell metabolism, only 1 or 2 doses were tested specific flavonoids evaluated in isolation. A dose-response study is needed to comprehensively identify whether level that modulates activity associated dietary supplemental levels red (RR) tea. We if RR stimulates mineralization...
Sympathetic tone may influence force potentiation, that is, the stimulation-induced increase in skeletal muscle mechanical function associated with myosin phosphorylation, although mechanism for this effect remains unknown. The purpose of study was to examine epinephrine on concentric twitch potentiation wild-type and light-chain kinase devoid mouse (skMLCK
ABSTRACT Sclerostin is an inhibitor of the osteogenic Wnt/β-catenin signaling pathway that also has endocrine role in regulating adipocyte differentiation and metabolism. Additionally, subcutaneous white adipose tissue (scWAT) sclerostin content decreases following exercise training (EXT). Therefore, we hypothesized EXT-induced reductions may play a adaptations body composition whole-body To test this hypothesis, 10-week-old male C57BL/6J mice were either sedentary (SED) or performing 1 hour...
Many human studies suggest a benefit of tea consumption on bone health. The study objective was to compare the ability different types promote mineralization. Saos-2 cells underwent mineralization (5 days) in presence (white: WT, green: GT, black: BT, green rooibos: GR, or red RR; 1 μg/mL polyphenols) control. Total polyphenol content (TPC, Folin-Ciocalteu's reagent), antioxidant capacity (2,2-diphenyl-1-picrylhydrazyl [DPPH] scavenging), (Alizarin Red staining), gene expression quantitative...
Microcomputed tomography (μCT) is an imaging technology to assess bone microarchitecture, a determinant of strength. When measured in vivo, μCT exposes the skeletal site interest dose radiation, addition nearby muscles as well. Therefore, aim this study was determine effects repeated radiation exposure from vivo on muscle health – specifically, morphometrics, contractile function, and enzyme activity. This exposed right hind limb female mice either low (26 cGy) or moderate (46 dose, at 2, 4,...
The isometric potentiation associated with myosin phosphorylation is force dependent. purpose of this study was to assess the influence a pre‐existing period on concentric displayed by mouse muscles and without ability phosphorylate myosin. We tested (ISO) (CON) potentiation, as well after (ISO‐CON), in from wildtype (WT) skeletal light chain kinase deficient (skMLCK ‐/‐ ) mice. A conditioning stimulus increased (i.e. potentiated) mean ISO‐CON CON conditions 1.31±0.02 1.35±0.02 1.19±0.02...