A5025737589- Esophageal and GI Pathology
- Tracheal and airway disorders
- Neonatal Respiratory Health Research
- Tissue Engineering and Regenerative Medicine
- Congenital Diaphragmatic Hernia Studies
- Extracellular vesicles in disease
- Mesenchymal stem cell research
- Renal and related cancers
- Adipokines, Inflammation, and Metabolic Diseases
- Respiratory Support and Mechanisms
- Nicotinic Acetylcholine Receptors Study
- Synthesis and Biological Evaluation
- Diabetes and associated disorders
- Congenital heart defects research
- Polyamine Metabolism and Applications
- Connective tissue disorders research
- Biochemical Analysis and Sensing Techniques
- Smoking Behavior and Cessation
- Electrospun Nanofibers in Biomedical Applications
- Foreign Body Medical Cases
- Pluripotent Stem Cells Research
- Adipose Tissue and Metabolism
UConn Health
2019-2024
University of Connecticut
2013-2022
Connecticut Children's Medical Center
2019
Abstract Background Despite advances in neonatal care, the incidence of Bronchopulmonary Dysplasia (BPD) remains high among preterm infants. Human induced pluripotent stem cells (hiPSCs) have shown promise repairing injury animal BPD models. Evidence suggests they exert their effects via paracrine mechanisms. We aim herein to assess effectiveness extracellular vesicles (EVs) derived from hiPSCs and alveolar progenies (diPSCs) attenuating hyperoxic a lung explant model. Methods Murine lobes...
Pediatric patients suffering from long gap esophageal defects or injuries are in desperate need of innovative treatment options. Our study demonstrates that two different cell sources can adhere to and proliferate on a retrievable synthetic scaffold. In feasibility testing translational applicability, these seeded scaffolds were implanted into piglets demonstrated regeneration.Either porcine epithelial cells amniotic fluid was obtained cultured 3 dimensions polyurethane scaffold (Biostage)....
Diseases of the esophagus, damage esophagus due to injury or congenital defects during fetal esophageal development, i.e., atresia (EA), typically require surgical intervention restore continuity. The development tissue engineered tubular structures would improve treatment options for these conditions by providing an alternative that is organ sparing and can be manufactured fit exact dimensions defect. An autologous Cellspan Esophageal ImplantTM (CEI) was surgically implanted into piglets...
Considerable work has gone into creating cell therapies from induced pluripotent stem cells (iPSCs) since their discovery just over a decade ago. However, comparatively little research been done concerning the safety of iPSCs and progeny specifically mechanisms governing teratogenicity. The aim this study was to ascertain at what developmental phase undergoing differentiation an alveolar-like phenotype lose capacity form teratoma uncover potential responsible. were differentiated using...
Smokeless tobacco products have been associated with increased risks of oro-pharyngeal cancers, due in part to the presence tobacco-specific nitrosamines (TSNAs) such as 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). These potent carcinogens are formed during curing and a result direct nitrosation reactions that occur oral cavity. In current work we describe isolation characterization hybridoma secreting high-affinity, NNK-specific monoclonal antibody. A structurally-related benzoyl...
BackgroundPreclinical studies demonstrate that tissue engineering and patient-derived stem cells can regenerate tissue. The goal of this study was to determine whether from esophageal atresia patients (EA) could be utilized for purpose.MethodsAdipose obtained control, long gap (LGEA) patients. Mesenchymal (MSCs) were isolated, expanded, characterized seeded onto tubular scaffolds 6 days. Scaffolds viability, gene expression cytokine production.ResultsThe average weight the EA LGEA 145.8mg...
Abstract Esophageal atresia (EA) is a congenital defect in which the esophagus not fully connected. Current treatments to bring together long gaps between have very high complication rates and replacement tissue does possess correct structure or physiologic properties. Tissue engineering regenerative medicine been suggested as new patient-specific treatment for EA utilizing patient's own cells tubular scaffold. Our group recently published on successful regeneration of an using retrievable...