A5050655999- Congenital Diaphragmatic Hernia Studies
- Neonatal Respiratory Health Research
- Congenital Anomalies and Fetal Surgery
- Prenatal Screening and Diagnostics
- Spinal Dysraphism and Malformations
- Virus-based gene therapy research
- Cerebrospinal fluid and hydrocephalus
- Tissue Engineering and Regenerative Medicine
- Intestinal Malrotation and Obstruction Disorders
- Muscle Physiology and Disorders
- Pediatric Hepatobiliary Diseases and Treatments
- RNA Interference and Gene Delivery
- Surgical Simulation and Training
- Opioid Use Disorder Treatment
- Gallbladder and Bile Duct Disorders
- COVID-19 Impact on Reproduction
- Airway Management and Intubation Techniques
- Anesthesia and Pain Management
- Cardiac, Anesthesia and Surgical Outcomes
- Appendicitis Diagnosis and Management
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Gastrointestinal disorders and treatments
- Esophageal and GI Pathology
- Preterm Birth and Chorioamnionitis
Yale University
2016-2025
Yale New Haven Hospital
2017-2024
Hartwell Foundation
2022
Case Western Reserve University
2022
Chase Acute Outpatients Hospital
2021
University Medical Center
2020
Yale Cancer Center
2020
University Hospital and Clinics
2020
Children's Center
2010-2014
Children's Hospital of Philadelphia
2004-2014
Genetic diseases can be diagnosed early during pregnancy, but many monogenic disorders continue to cause considerable neonatal and pediatric morbidity mortality. Early intervention through intrauterine gene editing, however, could correct the genetic defect, potentially allowing for normal organ development, functional disease improvement, or cure. Here we demonstrate safe intravenous intra-amniotic administration of polymeric nanoparticles fetal mouse tissues at selected gestational ages...
Matrix metalloproteinase activity is instrumental in processes of cellular invasion. The interstitial invasion endothelial cells during angiogenesis accompanied by up-regulation several matrix metalloproteinases, including membrane type 1 (MT1-MMP). In this study, we show that stimulated to undergo a three-dimensional extracellular environment increase production the transcription factor Egr-1. Increased binding Egr-1 MT1-MMP promoter correlates with enhanced transcriptional activity,...
Structural fetal diseases, such as congenital diaphragmatic hernia (CDH) can be diagnosed prenatally. Neonates with CDH are healthy in utero gas exchange is managed by the placenta, but impaired lung function results critical illness from time a baby takes its first breath. MicroRNA (miR) 200b and downstream targets TGF-β pathway critically involved branching morphogenesis. Here, we characterize expression of miR200b at different gestational times using rat model CDH. Fetal rats deficient...
In utero gene editing has the potential to modify disease causing genes in multiple developing tissues before birth, possibly allowing for normal organ development, improvement, and conceivably, cure. cystic fibrosis (CF), a that arises from mutations transmembrane conductance regulator (
Efficient gene transfer to muscle stem cells (satellite cells) has not been achieved despite broad transduction of skeletal by systemically administered adeno-associated virus serotype 2/9 (AAV-9) in mice. We hypothesized that cellular migration during fetal development would make satellite accessible for following utero intravascular injection. injected AAV-9 encoding green fluorescent protein (GFP) marker into the vascular space mice ranging ages from post-coital day 12 (E12) postnatal 1...
Presently, in vivo methods to efficiently and broadly transduce all major cell types throughout both the central (CNS) peripheral adult nervous system (PNS) are lacking. In this study, we hypothesized that during early fetal development neural populations, including stem cells (NSCs), may be accessible for gene transfer via open groove. To test hypothesis, injected lentiviral vectors encoding a green fluorescent protein (GFP) marker into murine amniotic cavity at embryonic day 8. This method...
Despite a national decrease in emergency department visits the United States during first 10 months of pandemic, preliminary Consumer Product Safety Commission data indicate increased firework-related injuries. We hypothesized an increase injuries 2020 compared to years prior related corresponding consumer firework sales.The National Electronic Injury Surveillance System (NEISS) was queried from 2018 for cases with product codes 1313 (firework injury) and narratives containing "fireworks"....
Background Advances in Molecular Therapy have made gene editing through systemic or topical administration of reagents a feasible strategy to treat genetic diseases rational manner. Encapsulation therapeutic agents nanoparticles can improve intracellular delivery agents, provided that the are efficiently taken up within target cells. In prior work we had established proof-of-principle carrying mediate site-specific fetal and adult animals vivo results functional disease improvement rodent...
Infants prenatally suspected of having a choledochal cyst (CDC) typically undergo ultrasound imaging shortly after birth. This study sought to evaluate features on the initial postnatal (IPU) that could identify newborns at risk for early complications.Following IRB approval, patients from four US fetal centers with prenatal suspicion CDC and 2000 2017 were reviewed. Imaging clinical courses assessed.Forty-two had ultrasounds suspicious CDC. Nineteen (45.2%) excluded due diagnostic revision...
To compare institutional practice patterns for gastrostomy tube placement in neonates with duodenal atresia (DA) and trisomy 21.A retrospective review of the Pediatric Health Information System (PHIS) from 2015 to 2018 identified infants <10 days old ICD-10 diagnostic codes DA 21, addition procedure an intestinal bypass or duodenoduodenostomy. This cohort was then queried associated co-morbidities.Two hundred nine were DA, bypass. Fifty-seven (27%) underwent placement. Baseline...