A5074470733- Ultrasound and Hyperthermia Applications
- Vagus Nerve Stimulation Research
- Microfluidic and Capillary Electrophoresis Applications
- Heart Rate Variability and Autonomic Control
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Bio-sensing Technologies
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Electrowetting and Microfluidic Technologies
- 3D Printing in Biomedical Research
- Cancer Genomics and Diagnostics
- Bacterial Identification and Susceptibility Testing
- Pain Management and Treatment
- Neuroscience and Neural Engineering
- Infrared Thermography in Medicine
- Laser Applications in Dentistry and Medicine
- Antibiotic Resistance in Bacteria
- EEG and Brain-Computer Interfaces
- Quantum Dots Synthesis And Properties
- Single-cell and spatial transcriptomics
- Wound Healing and Treatments
- Microbial Inactivation Methods
- Monoclonal and Polyclonal Antibodies Research
- Osteoarthritis Treatment and Mechanisms
- Nanopore and Nanochannel Transport Studies
Rensselaer Polytechnic Institute
2025
General Electric (United States)
2015-2024
Division of Undergraduate Education
2024
GE Global Research (United States)
2016-2023
Ochsner Medical Center
2022
Feinstein Institute for Medical Research
2021
General Electric (Israel)
2015
Johns Hopkins University
2005-2012
Masonic Medical Research Laboratory
2011
Abstract Tools for noninvasively modulating neural signaling in peripheral organs will advance the study of nerves and their effect on homeostasis disease. Herein, we demonstrate a noninvasive method to modulate specific pathways within using ultrasound (U/S). U/S is first applied spleen cholinergic anti-inflammatory pathway (CAP), US stimulation shown reduce cytokine response endotoxin same levels as implant-based vagus nerve (VNS). Next, hepatic that regulate blood glucose effective VNS...
Short QT syndrome (SQTS) is a genetically determined ion-channel disorder, which may cause malignant tachyarrhythmias and sudden cardiac death. Thus far, mutations in five different genes encoding potassium calcium channel subunits have been reported. We present, for the first time, novel loss-of-function mutation coding an L-type subunit. The electrocardiogram of affected member single family revealed interval 317 ms (QTc 329 ms) with tall, narrow, symmetrical T-waves. Invasive...
Mesenchymal stem cells (MSCs) are potentially useful for musculoskeletal tissue engineering. However, controlling MSC differentiation and formation in vivo remains a challenge. There is significant need well-defined efficient protocols directing behaviors vivo. We hypothesize that morphogenetic signals from chondrocytes may regulate differentiation. In micromass culture of MSCs, incubation with chondrocyte-conditioned medium (CCM) significantly enhanced the production cartilage specific...
In this article we present a novel droplet microfluidic chip enabling amplification-free detection of single pathogenic cells. The device streamlines multiple functionalities to carry out sample digitization, cell lysis, probe–target hybridization for subsequent fluorescent detection. A peptide nucleic acid fluorescence resonance energy transfer probe (PNA beacon) is used detect 16S rRNA in Initially the sensitivity and quantification abilities platform are tested using synthetic target...
Inflammation is pathogenically implicated in pulmonary arterial hypertension; however, it has not been adequately targeted therapeutically. We investigated whether neuromodulation of an anti-inflammatory neuroimmune pathway involving the splenic nerve using noninvasive, focused ultrasound stimulation spleen (sFUS) can improve experimental hypertension.
This paper describes the fabrication and application of microfluidic devices containing collagen vitrigel (CV) used as both a functional sacrificial cell growth substrate for development corneal microtissue patches. Within device, vacuum fixation CV in dehydrated state enables quick integration with standard multilayer soft lithographic techniques, while on-chip rehydration results gel-like culture. Fluidic connectivity to apical basal side permits bilayered culture epithelium supporting...
Empiric broad-spectrum antimicrobial treatments of urinary tract infections (UTIs) have contributed to widespread resistance. Clinical adoption evidence-based necessitates rapid diagnostic methods for pathogen identification (ID) and susceptibility testing (AST) with minimal sample preparation. In response, a microfluidic droplet-based platform is developed achieving both ID AST from urine samples within 30 min. this platform, fluorogenic hybridization probes are utilized detect 16S rRNA...
We demonstrate single biomolecule detection and quantification within sub-nanolitre droplets through application of Cylindrical Illumination Confocal Spectroscopy (CICS) droplet confinement a retractable microfluidic constriction.
Abstract Obesity, a growing health concern, is associated with an increased risk of morbidity and mortality. Chronic low-grade inflammation implicated in obesity-driven metabolic complications. Peripheral focused ultrasound stimulation (pFUS) emerging non-invasive technology that modulates inflammation. Here, we reasoned the liver may alleviate obesity-related other comorbidities. After 8 weeks on high-fat high-carbohydrate “Western” diet, C57BL/6J mice were subjected to either sham or at...
We report on a hand-held multiplexed impedance sensor system and show evidence for impedance-based monitoring of the growth single bacterium.
We performed on-chip DNA methylation analysis using methylation-specific PCR (MSP) within an arrayed micro droplet-in-oil platform that is designed for more practical application of microfluidic droplet technologies in clinical applications. Unique features this ready-to-use device include primers are pre-deposited into open micro-reaction chambers and use the oil phase as a companion fluid both sample actuation compartmentalization. These technical advantages allow infusion minute amounts...
Bloodstream infections are a significant cause of morbidity and mortality worldwide. The rapid initiation effective antibiotic treatment is critical for patients with bloodstream infections. However, the diagnosis bloodborne pathogens largely complicated by matrix effect blood lengthy tube culture procedure. Here we report culture-free workflow isolation enrichment bacterial from whole single-cell antimicrobial susceptibility testing (AST). A dextran sedimentation step reduces concentration...
Interfaces between the nervous and immune systems have been shown essential for coordination regulation of responses. Non-invasive ultrasound stimulation targeted to spleen has recently capable activating one such interface, splenic cholinergic anti-inflammatory pathway (CAP). Over past decade, CAP other neuroimmune pathways activated using implanted nerve stimulators tested prevent cytokine release inflammation. However, studies typically performed in models severe, systemic (e.g.,...
ObjectivesLow-intensity, focused ultrasound (FUS) is an emerging noninvasive neuromodulation approach, with improved spatial and temporal resolution penetration depth compared to other electrical stimulation strategies. FUS has been used modulate circuits in the brain peripheral nervous system, however, its potential spinal unclear. In this study, we assessed effect of trans-spinal (tsFUS) on reflexes healthy rats.Materials MethodstsFUS targeting different segments was delivered for 1...
In vitro models of the alveolo-pulmonary barrier consist microvascular endothelial cells and alveolar epithelial cultured on opposing sides synthetic porous membranes. However, these simple do not reflect physiological microenvironment pulmonary cells, wherein are exposed to a complex milieu mechanical soluble stimuli. this report, we studied (A549) (HMEC-1) within varying microfluidic environments as first step towards building analog gas-exchange interface. We fabricated...
We used inline, micro-evaporators to concentrate and transport DNA targets a nanoliter single molecule fluorescence detection chamber for subsequent molecular beacon probe hybridization analysis. This use of solvent removal as unique means target in microanalytical platform led greater than 5000-fold concentration enhancement limits that pushed below the femtomolar barrier commonly reported using confocal detection. simple microliter-to-nanoliter interconnect counting analysis resolved...