A5054285143- Ultrasound and Hyperthermia Applications
- Vagus Nerve Stimulation Research
- Single-cell and spatial transcriptomics
- Polymer crystallization and properties
- Heart Rate Variability and Autonomic Control
- Radiomics and Machine Learning in Medical Imaging
- Glioma Diagnosis and Treatment
- Alzheimer's disease research and treatments
- Cancer Research and Treatments
- Bioinformatics and Genomic Networks
- Material Dynamics and Properties
- Colorectal Cancer Treatments and Studies
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Cell Image Analysis Techniques
- Advanced Polymer Synthesis and Characterization
- Advanced Physical and Chemical Molecular Interactions
- Cancer Genomics and Diagnostics
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- Material Properties and Applications
- Thermodynamic properties of mixtures
- Molecular Biology Techniques and Applications
- Rheology and Fluid Dynamics Studies
- Monoclonal and Polyclonal Antibodies Research
GE Global Research (United States)
2013-2024
General Electric (Israel)
2023
General Electric (United States)
1994-2023
University of California, San Francisco
2011-2016
San Francisco General Hospital
2011
University of Bonn
2008
Pennsylvania State University
1990-1997
Children's Hospital of Winnipeg
1962
University of Manitoba
1962
Abstract Tools for noninvasively modulating neural signaling in peripheral organs will advance the study of nerves and their effect on homeostasis disease. Herein, we demonstrate a noninvasive method to modulate specific pathways within using ultrasound (U/S). U/S is first applied spleen cholinergic anti-inflammatory pathway (CAP), US stimulation shown reduce cytokine response endotoxin same levels as implant-based vagus nerve (VNS). Next, hepatic that regulate blood glucose effective VNS...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEffect of hydrogen bonding on the enthalpy mixing and composition dependence glass transition temperature in polymer blendsPaul C. Painter, John F. Graf, Michael M. ColemanCite this: Macromolecules 1991, 24, 20, 5630–5638Publication Date (Print):September 1, 1991Publication History Published online1 May 2002Published inissue 1 September 1991https://pubs.acs.org/doi/10.1021/ma00020a023https://doi.org/10.1021/ma00020a023research-articleACS...
To determine whether differentially methylated CpGs in synovium-derived fibroblast-like synoviocytes (FLS) of patients with rheumatoid arthritis (RA) were also RA peripheral blood (PB) samples.For this study, 371 genome-wide DNA methylation profiles measured using Illumina HumanMethylation450 BeadChips PB samples from 63 and 31 unaffected control subjects, specifically the cell subsets CD14+ monocytes, CD19+ B cells, CD4+ memory T naive cells.Of 5,532 hypermethylated FLS candidate CpGs,...
Abstract Background Measures of neocortical amyloid burden (NAB) identify individuals who are at substantially greater risk developing Alzheimer's disease (AD). Blood‐based biomarkers predicting NAB would have great utility for the enrichment AD clinical trials, including large‐scale prevention trials. Methods Nontargeted proteomic discovery was applied to 78 subjects from Australian Imaging, Biomarkers and Lifestyle Flagship Study Ageing with a range values. Technical independent...
Increasingly, clinical trials for Alzheimer's disease (AD) are being conducted earlier in the phase and with biomarker confirmation using vivo amyloid PET imaging or CSF tau Aβ measures to quantify pathology. However, making such a pre-clinical AD diagnosis is relatively costly screening failure rate likely be high. Having blood-based marker that would reduce costs accelerate through identifying potential participants substantial advance. In order seek candidate biomarker, discovery...
Spectroscopic evidence is presented to indicate that local screening effects are important in polymer mixtures. Essentially, a segment has locally higher concentration of like segments because this factor. A simple modification existing theories account for screening.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEquilibrium constants and the prediction of phase behavior for phenoxy blends with aliphatic polyestersMichael M. Coleman, Xiaoming Yang, Paul C. Painter, John F. GrafCite this: Macromolecules 1992, 25, 17, 4414–4424Publication Date (Print):August 1, 1992Publication History Published online1 May 2002Published inissue 1 August 1992https://pubs.acs.org/doi/10.1021/ma00043a027https://doi.org/10.1021/ma00043a027research-articleACS PublicationsRequest...
Abstract Background: Identification of the consensus molecular subtypes (CMS) opened significant potential for understanding tumor biology and intertumoral heterogeneity colorectal cancer (CRC). Molecular subtyping in CRC traditionally relies on bulk transcriptomics. However, single-cell transcriptomics studies have shown that tumors may be composed cells displaying different CMS traits, suggesting additional intratumoral heterogeneity. Methods: To investigate at protein level, we...
A Flory lattice model is used to obtain an expression for the free energy of mixing polymers that hydrogen bond. The derivation based on determination probability a mixture nonhydrogen bonded chains would spontaneously occur in configuration equivalent system. Our result has three parts. usual contribution from covalent chains, bonds equal be obtained if segments were not covalently linked, and ‘‘correction’’ term accounts excess entropy introduced by second term. consequence this there...
Objective.Patients with RA have systemic inflammation and increased risk of cardiovascular (CV) events, including thrombosis.Levels fibrinogen, a pro-thrombotic protein predictive value for CV disease (CVD), are elevated during inflammation.We compared circulating fibrinogen levels in patients healthy controls evaluated the relationship measures activity.Methods.Patients were recruited at University California, San Francisco (UCSF).Disease activity was using standard composite...
Glioma is recognized to be a highly heterogeneous CNS malignancy, whose diverse cellular composition and interactions have not been well characterized. To gain new clinical- biological-insights into the genetically-bifurcated IDH1 mutant (mt) vs wildtype (wt) forms of glioma, we integrated data from protein, genomic MR imaging 20 treatment-naïve glioma cases 16 recurrent GBM cases. Multiplexed immunofluorescence (MxIF) was used generate single cell for 43 protein markers representing all...
Interfaces between the nervous and immune systems have been shown essential for coordination regulation of responses. Non-invasive ultrasound stimulation targeted to spleen has recently capable activating one such interface, splenic cholinergic anti-inflammatory pathway (CAP). Over past decade, CAP other neuroimmune pathways activated using implanted nerve stimulators tested prevent cytokine release inflammation. However, studies typically performed in models severe, systemic (e.g.,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFormation of molecular composites through hydrogen-bonding interactionsPaul C. Painter, Wei Long Tang, John F. Graf, Barry Thomson, and Michael M. ColemanCite this: Macromolecules 1991, 24, 13, 3929–3936Publication Date (Print):June 1, 1991Publication History Published online1 May 2002Published inissue 1 June 1991https://pubs.acs.org/doi/10.1021/ma00013a029https://doi.org/10.1021/ma00013a029research-articleACS PublicationsRequest reuse...
Tumor heterogeneity can manifest itself by sub-populations of cells having distinct phenotypic profiles expressed as diverse molecular, morphological and spatial distributions. This inherent poses challenges in terms diagnosis, prognosis efficient treatment. Consequently, tools techniques are being developed to properly characterize quantify tumor heterogeneity. Multiplexed immunofluorescence (MxIF) is one such technology that offers molecular insight into both inter-individual intratumor It...
Abstract Cancer cells’ ability to inhibit apoptosis is key malignant transformation and limits response therapy. Here, we performed multiplexed immunofluorescence analysis on tissue microarrays with 373 cores from 168 patients, segmentation of 2.4 million individual cells, quantification 18 cell lineage proteins. We identified an enrichment for BCL2 in immune, BAK, SMAC, XIAP cancer cells. Ordinary differential equation-based modeling sensitivity at single-cell resolution was conducted atlas...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCoal solubility and swelling. 1. Solubility parameters for coal the Flory .chi. parameterPaul C. Painter, John Graf, Michael M. ColemanCite this: Energy Fuels 1990, 4, 379–384Publication Date (Print):July 1, 1990Publication History Published online1 May 2002Published inissue 1 July 1990https://pubs.acs.org/doi/10.1021/ef00022a008https://doi.org/10.1021/ef00022a008research-articleACS PublicationsRequest reuse permissionsArticle...
Multiplexed immunofluorescence bioimaging of single-cells and their spatial organization in tissue holds great promise to the development future precision diagnostics therapeutics. Current multiplexing pipelines typically involve multiple rounds staining across slides. This introduces experimental batch effects that can hide underlying biological signal. It is important have robust algorithms correct for while not introducing biases into data. Performance data normalization methods vary...
Colorectal cancer (CRC) has one of the highest incidences and mortality rates. In stage III, postoperative chemotherapy benefits <20% patients, while more than 50% will develop distant metastases. Biomarkers for identification patients at increased risk disease recurrence following adjuvant are currently lacking. this study, we assessed immune signatures in tumor microenvironment (TME) using an situ multiplexed immunofluorescence imaging single-cell analysis technology (Cell DIVETM)...
We developed a detailed, whole-body physiologically based pharmacokinetic (PBPK) modeling tool for calculating the distribution of pharmaceutical agents in various tissues and organs human or animal as function time. Ordinary differential equations (ODEs) represent circulation body fluids through at macroscopic level, biological transport mechanisms biotransformations within cells their organelles molecular scale. Each major organ is modeled composed one more tissues. Tissues are made up...