A5019474699- Single-cell and spatial transcriptomics
- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
- Immunotherapy and Immune Responses
- Inflammatory Bowel Disease
- Cell Image Analysis Techniques
- Colorectal Cancer Treatments and Studies
- Immune cells in cancer
- Advanced Biosensing Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Bladder and Urothelial Cancer Treatments
- AI in cancer detection
- Radiomics and Machine Learning in Medical Imaging
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Genetic factors in colorectal cancer
- Eosinophilic Esophagitis
- Breast Cancer Treatment Studies
- Hemoglobinopathies and Related Disorders
- Selenium in Biological Systems
- Prostate Cancer Treatment and Research
- Cancer, Hypoxia, and Metabolism
- Adolescent and Pediatric Healthcare
- Microscopic Colitis
- Cancer Genomics and Diagnostics
Louisiana State University Health Sciences Center New Orleans
2025
GE Global Research (United States)
2013-2024
Texas Tech University Health Sciences Center
2024
General Electric (Spain)
2024
Oklahoma City University
2024
University of Oklahoma
2024
Texas Tech University
2024
Core Research Center
2022
General Electric (Israel)
2022
General Electric (United States)
2019
Abstract The reactivity of human lymphocytes, separated from peripheral blood by procedures involving sedimentation through plasmagel and settling on a glass surface, survival or growth melanoma tumor cells is reported. Assay was either colony inhibition in dilute agar (GEL) technique microcytotoxicity assay (MC). In 54 experiments with lymphocytes normal donors, cell viability, as compared to medium alone, seen 25 (46.3%) times, stimulation observed seven (12.9%) times no effect 22 (40.7%)...
Patients suffering from ulcerative colitis (UC) exhibit chronic colonic inflammation caused by a dysregulated mucosal immune response and epithelial barrier disruption. Th2 cytokines, including IL-13, have been implicated in the pathogenesis of UC. IL-13 induces phosphorylation STAT6, we previously demonstrated increased p-STAT6 children with In this study, investigated role STAT6 oxazolone colitis, murine model UC, inducing STAT6-deficient (STAT6(-/-)) wild type (WT) mice. We observed cell,...
The phenotypic diversity of cancer results from genetic and nongenetic factors. Most studies heterogeneity have focused on DNA alterations, as technologies for proteomic measurements in clinical specimen are currently less advanced. Here, we used a multiplexed immunofluorescence staining platform to measure the expression 27 proteins at single-cell level formalin-fixed paraffin-embedded samples treatment-naive stage II/III human breast cancer. Unsupervised clustering protein data 638,577...
Inflammatory bowel disease (IBD) results from genetic susceptibility, gut microbiome, and environmental factors. Diet, one modifiable factor, has been linked to the increased prevalence of IBD. This study aimed evaluate a potential association between food deserts severity at diagnosis. retrospective included newly diagnosed IBD patients (ages 2 21 years age; January 1, 2019, December 31, 2021). The United States Department Agriculture (USDA's) Food Access Research Atlas was used determine...
Sickle cell disease (SCD) conveys a high risk of neuropsychological impairment due to chronic anemia, hypoxemia, cerebrovascular ischemia and stroke, in addition causing pain other biomedical complications. Few families accessed testing via the traditional referral system. In this paper, we describe successful alternative strategy for addressing cognitive behavioral needs youth with SCD.The Cell Disease Program Learning Neuropsychological Evaluation (SCD-PLANE) was embedded 2007 into SCD...
Introduction: Prostate cancer is a highly heterogeneous disease, presenting varying levels of aggressiveness and response to treatment. Angiogenesis one the hallmarks cancer, providing oxygen nutrient supply tumors. Micro vessel density has previously been correlated with higher Gleason score poor prognosis. Manual segmentation blood vessels (BVs) In microscopy images challenging, time consuming may be prone inter-rater variabilities. this study, an automated pipeline presented for BV...
Abstract Background Loss of AZGP1 expression is a biomarker associated with progression to castration resistance, development metastasis, and poor disease-specific survival in prostate cancer. However, high cells cancer has been reported increase proliferation invasion. The exact role remains elusive. Method knockout overexpressing were generated using lentiviral system. effects under- or over-expression evaluated by vitro cell proliferation, migration, invasion assays. Heterozygous ± mice...
Multiplexed immunofluorescence bioimaging of single-cells and their spatial organization in tissue holds great promise to the development future precision diagnostics therapeutics. Current multiplexing pipelines typically involve multiple rounds staining across slides. This introduces experimental batch effects that can hide underlying biological signal. It is important have robust algorithms correct for while not introducing biases into data. Performance data normalization methods vary...
<h3>Objective</h3> The myeloid translocation genes (MTGs) are transcriptional corepressors with both <i>Mtg8<sup>−/−</sup></i> and <i>Mtgr1<sup>−/−</sup></i> mice showing developmental and/or differentiation defects in the intestine. We sought to determine role of MTG16 intestinal integrity. <h3>Methods</h3> Baseline stress induced colonic phenotypes were examined <i>Mtg16<sup>−/−</sup></i> mice. To unmask phenotypes, we treated dextran sodium sulphate (DSS) or infected them <i>Citrobacter...
We report here the case of youngest patient with adenomyomatosis gallbladder in a female infant diagnosed at 4 months age. This diagnosis was made based on characteristic ultrasonography findings that undergoing routine surveillance for suspected clinical Beckwith-Wiedemann syndrome. The remains asymptomatic and currently no surgical interventions have been needed. review pathophysiology ultrasonographic this rare condition present comparison only other four pediatric cases gallbladder.
Standard clinicopathological parameters (age, growth pattern, tumor size, margin status, and grade) have been shown to limited value in predicting recurrence ductal carcinoma situ (DCIS) patients. Early accurate prediction would facilitate a more aggressive treatment policy for high-risk patients (mastectomy or adjuvant radiation therapy), simultaneously reduce over-treatment of low-risk Generative adversarial networks (GAN) are class DL models which two neural networks, generator...
Abstract Mapping the human body at single cell resolution in three-dimensions (3D) is an important step toward a “digital twin” model that captures structure and dynamics of cell-cell interactions. Current 3D imaging methods suffer from low are limited their ability to distinguish types spatial relationships. We present novel workflow: MATRICS-A ( M ultiplexed Im ge T hree-D R econstruction I ntegrated C ell S patial - A nalysis) generates map cells multiplexed images calculates type...
MTG16 is a member of the myeloid translocation gene (MTG) family transcriptional corepressors. While MTGs were originally identified in chromosomal translocations acute leukemia, recent studies have uncovered role intestinal biology. For example, Mtg16-/- mice increased proliferation and are more sensitive to injury colitis models. also underexpressed patients with moderate/severe ulcerative colitis. Based on these findings, we postulated that might protect against colitis-associated...
BACKGROUND: Mucinous rectal cancer is associated with a higher incidence of microsatellite instability, and poorer response to neoadjuvant chemoradiotherapy compared other subtypes adenocarcinoma. Immune checkpoint inhibitors are an emerging family anti-cancer therapeutics highly variable outcomes in colorectal cancer. Though the immune landscape mucinous has not been fully explored, presence mucin thought act as barrier preventing cell infiltration. OBJECTIVE: The aim this study was...
Colorectal cancer (CRC) is one of the most frequently occurring cancers, but prognostic biomarkers identifying patients at risk recurrence are still lacking. In this study, we aimed to investigate in more detail spatial relationship between intratumoural T cells, and cell hallmarks, as stage III colorectal patients. We conducted multiplexed imaging 56 protein markers single resolution on resected fixed tissue from CRC who received adjuvant 5-fluorouracil-based chemotherapy. Images underwent...
Abstract Colorectal cancer (CRC) is one of the most frequently occurring cancers, but prognostic biomarkers identifying patients at risk recurrence are still lacking. In this study, we aimed to investigate in more detail spatial relationship between intratumoural T cells, and cell hallmarks as stage III colorectal patients. We conducted multiplexed imaging 56 protein markers single‐cell resolution on resected fixed tissue from CRC who received adjuvant 5‐fluorouracil (5FU)‐based...
Abstract Background In Alzheimer’s disease (AD), specific brain regions become vulnerable to pathology while others remain resilient. New methods of imaging such as highly multiplexed immunofluorescence (MxIF) provide an abundance spatial information, analytical techniques like machine learning (ML) can address questions cellular contributors this regional vulnerability. Method We performed MxIF staining for 26 markers and compared postmortem human samples from AD‐susceptible area, the...