A5024777354- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Research on Leishmaniasis Studies
- Cancer Cells and Metastasis
- Estrogen and related hormone effects
- Kruppel-like factors research
- Trypanosoma species research and implications
- Genomics and Chromatin Dynamics
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- Redox biology and oxidative stress
- Wnt/β-catenin signaling in development and cancer
- Radiology practices and education
- Shoulder Injury and Treatment
- Vitamin D Research Studies
- Barrier Structure and Function Studies
- Nerve Injury and Rehabilitation
- Cancer-related Molecular Pathways
- interferon and immune responses
- Effects of Radiation Exposure
- RNA and protein synthesis mechanisms
- Biochemical and Molecular Research
St Bartholomew's Hospital
2024
Mitchell Institute
2024
Vardhman Mahavir Medical College & Safdarjung Hospital
2008-2023
Indian Spinal Injuries Centre
2022
Safdarjang Hospital
2009-2021
Meharry Medical College
2007-2020
Vanderbilt University Medical Center
2011-2018
Vanderbilt University
2014-2018
University Gastroenterology
2017
VA Tennessee Valley Healthcare System
2015
Clinical resistance to pentavalent antimonial compounds has long been recognized as a major problem in the treatment of visceral leishmaniasis India. However, mechanisms natural are unclear. In this study, we observed that Leishmania donovani clinical isolates not responsive sodium stibogluconate showed antimony both vitro and vivo laboratory conditions. The resistant have increased levels intracellular thiols. This increase thiol was mediated by amplification gamma-glutamylcysteine...
Methamphetamine (METH) is a highly addictive and neurotoxic psychostimulant. Its use in humans often associated with neurocognitive impairment. Whether this due to long‐term deficits short‐term memory and/or hippocampal plasticity remains unclear. Recently, we reported that METH increases baseline synaptic transmission reduces LTP an ex vivo preparation of the CA1 region from young mice. In current study, tested hypothesis repeated regimen exposure adolescent mice decreases produces deficit...
UbcH5c, a member of the UbcH5 family protein ubiquitin conjugase E2 enzymes, is critical component biological processes in human cells, being initial ubiquitinating enzyme substrates like IκB, TP53, and cyclin D1. We report here that metastasis regulator SLUG inhibits expression UbcH5c directly through chromatin remodeling thus, among other downstream effects, elevates level D1, thus enhancing growth rates breast cancer cells. Overexpression SLUG-deficient cells significantly decreased...
<h3>Objective</h3> Blood vessel epicardial substance (BVES) is a tight junction-associated protein that regulates epithelial-mesenchymal states and underexpressed in epithelial malignancy. However, the functional impact of BVES loss on tumourigenesis unknown. Here we define vivo role colitis-associated cancer (CAC), its cellular function relevance to patients with IBD. <h3>Design</h3> We determined <i>BVES</i> promoter methylation status using an Infinium HumanMethylation450 array screen UC...
One of highly pathogenic breast cancer cell types are the triple negative (negative in expression estrogen, progesterone, and ERBB2 receptors) cells. These cells motile metastatic characterized by high levels metastasis regulator protein SLUG. Using isogenic systems we have shown here that motility these is directly correlated with SLUG Because epithelial/mesenchymal known to be negatively regulated catenin plakoglobin, postulated transcriptional repressor increases aggressive through...
Abstract Background BRCA2 gene expression is tightly regulated during the cell cycle in human breast cells. The of silenced at G0/G1 phase growth and de-silenced S/G2 phase. While studying activity promoter cancer cells, we discovered that this has bi-directional product reverse (a ZAR1-like protein, named ZAR2) silences forward cell. Standard techniques like synchronization by serum starvation, flow cytometry, N-terminal or C-terminal FLAG epitope-tagged protein expression,...
Blood vessel epicardial substance (BVES/Popdc1) is a junctional-associated transmembrane protein that underexpressed in number of malignancies and regulates epithelial-to-mesenchymal transition. We previously identified role for BVES regulation the Wnt pathway, modulator intestinal stem cell programs, but its small (SI) biology remains unexplored. hypothesized influences programs critical to SI homeostasis after radiation injury. At baseline, Bves(-/-) mice demonstrated increased crypt...
Myeloid translocation genes (MTGs) are transcriptional corepressors implicated in development, malignancy, differentiation, and stem cell function. While MTG16 loss renders mice sensitive to chemical colitis, the role of small intestine is unknown. Histological examination revealed that Mtg16 −/− have increased enterocyte proliferation goblet deficiency. After exposure radiation, exhibited crypt viability decreased apoptosis compared with wild-type (WT) mice. Flow cytometric...
The parasitic protozoan Leishmania invades mammalian macrophages to establish infection. We reported previously that manipulates the expression of several non-coding RNA genes (e.g. Alu RNA, B1 and signal recognition particle RNA) in favor establishment their infection phagolysosomes these cells (Ueda, Y., Chaudhuri, G. (2000) J. Biol. Chem. 275, 19428-19432; Misra, S., Tripathi, M. K., (2005) 280, 29364-29373). report here mechanism this down-regulation. found (ncRNA) are repressed by...
An impaired nitrergic system and altered redox signaling contribute to gastric dysmotility in diabetics. Our earlier studies show that NF-E2-related factor 2 (NRF2) phase II antioxidant enzymes play a vital role neuronal nitric oxide synthase (nNOS) function. This study aims investigate whether supplementation of sepiapterin (SEP), precursor for tetrahydrobiopterin (BH4) (a cofactor NOS) via the salvage pathway, restores systems balance spontaneous diabetic (DB) female rats. Twelve-week DB...
MTG16 is a member of the myeloid translocation gene (MTG) family transcriptional corepressors. While MTGs were originally identified in chromosomal translocations acute leukemia, recent studies have uncovered role intestinal biology. For example, Mtg16-/- mice increased proliferation and are more sensitive to injury colitis models. also underexpressed patients with moderate/severe ulcerative colitis. Based on these findings, we postulated that might protect against colitis-associated...
We have previously shown that the zinc finger transcription repressor SNAI2 (SLUG) represses tumor suppressor BRCA2-expression in non-dividing cells by binding to E2-box upstream of start site. However, it is unclear how proliferating breast cancer (BC) has higher oxidation state, overcome this repression. In study, we provide insight into mechanism de-silencing BRCA2 gene expression PRDX5A, which longest member peroxiredoxin5 family, cells.We used cell synchronization and DNA affinity...