A5017946798- Tuberculosis Research and Epidemiology
- Diagnosis and treatment of tuberculosis
- Mycobacterium research and diagnosis
- Infectious Diseases and Tuberculosis
- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Reproductive System and Pregnancy
- Inflammatory Bowel Disease
- Hormonal and reproductive studies
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Escherichia coli research studies
- Vitamin D Research Studies
- Neurotransmitter Receptor Influence on Behavior
- Microscopic Colitis
- Stress Responses and Cortisol
- Extracellular vesicles in disease
- PARP inhibition in cancer therapy
- Complement system in diseases
- Blood Coagulation and Thrombosis Mechanisms
- Adipose Tissue and Metabolism
- Eosinophilic Esophagitis
- Pharmacological Receptor Mechanisms and Effects
- Genetics, Aging, and Longevity in Model Organisms
- Parkinson's Disease Mechanisms and Treatments
Meharry Medical College
2010-2022
Allen Institute for Brain Science
2018
Icahn School of Medicine at Mount Sinai
2018
Vanderbilt University Medical Center
2017
The University of Texas Medical Branch at Galveston
2013
Czech Academy of Sciences, Institute of Microbiology
2012
All India Institute of Medical Sciences
2005-2011
National Institutes of Health
2005-2006
Indian Institute of Technology Roorkee
2004
Quaid-i-Azam University
1981
The duration and strength of the dopaminergic signal are regulated by dopamine transporter (DAT). Drug addiction neurodegenerative neuropsychiatric diseases have all been associated with altered DAT activity. membrane localization activity a number intracellular proteins. α-Synuclein, protein partner DAT, is implicated in disease drug addiction. Little known about regulatory mechanisms interaction between α-synuclein, cellular location this interaction, functional consequences on basal,...
Histopathological and mycobacteriological examinations have limited utility in the diagnosis of genital tuberculosis. In this double-blind study, 61 samples, consisting endometrial aspirates (EAs), biopsies (EBs) fluid from pouch Douglas (POD), 25 women suffering infertility were investigated for presence mpt64 gene Mycobacterium tuberculosis by PCR correlated with laparoscopic findings. demonstrated M. DNA 14 out patients (56.0 %), compared to one smear acid-fast bacilli (1.6 %) two...
The mechanisms by which exosomes (nano‐vesicular messengers of cells) are taken up recipient cells poorly understood. We hypothesized that histones associated with these nanoparticles the ligands facilitate their interaction cell surface syndecan‐4 ( SDC 4) to mediate uptake. show incubation fetuin‐A (exosome‐associated proteins) and mediates uptake normally not endocytosed. Similarly, hydroxyapatite‐nanoparticles incubated FNH ) internalized tumor cells, while alone FN not. , both move...
Abstract Cocaine exposure alters gene expression in the brain via methylation and acetylation of histones along with DNA. Recently, poly (ADP-ribose) polymerase-1 (PARP-1) catalyzed PARylation has been reported as an important regulator cocaine-mediated expression. In this study, we report that cellular microRNA “miR-125b” plays a key role for cocaine-induced PARP-1 Acute chronic cocaine resulted downregulation miR-125b concurrent upregulation dopaminergic neuronal cells nucleus accumbens...
Decreased energy production and increased oxidative stress are considered to be major contributors aging aging-associated pathologies. The role of mitochondrial calcium homeostasis has also been highlighted as an important factor affecting different pathological conditions. Here, we present evidence that loss a small protein Fus1 maintains results in premature aging, pathologies, decreased survival. We showed Fus1KO mice develop multiple early signs including lordokyphosis, lack vigor,...
This study was designed to test the utility of mRNA-based RT-PCR detect viable bacilli, indicating active tubercular involvement, and DNA-PCR present or past infection in diagnosis female genital tuberculosis (TB) infection. A total 200 subjects with complaints infertility were enrolled study. Multiple sampling done. One hundred forty-three endometrial aspirate (EA), 94 peritoneal fluid/peritoneal washing (PF/PW) six cornual biopsy (CB) specimens collected for using microscopy, culture,...
Abstract Glioblastomas (high‐grade astrocytomas) are highly aggressive brain tumors with poor prognosis and limited treatment options. In the present studies, we have defined role of fetuin‐A, a liver‐derived multifunctional serum protein, in growth an established glioblastoma cell line, LN 229. We hereby demonstrate that these cells synthesize ectopic fetuin‐A which supports their culture absence serum. demonstrated panel tissue microarray ( TMA ) glioblastomas also express fetuin‐A....
Fever of unknown origin (FUO) poses a diagnostic challenge to the clinicians, with differential diagnosis as varied neoplastic and infectious diseases. In developing countries, causes are responsible for more cases FUO, tuberculosis one main classic FUO. Disseminated negative pulmonary findings is problem. This study examines utility polymerase chain reaction (PCR) in samples bone marrow aspirate 85 patients presenting diverse clinical symptoms. Using primers specific Mycobacterium ,...
The microbiologic diagnosis of cutaneous tuberculosis is difficult because most lesions harbor only a small number mycobacteria that cannot usually be detected by staining for the organism or culture. Nucleic acid amplification tests based on polymerase chain reaction (PCR) are potentially useful in this situation.To evaluate utility mRNA PCR and DNA tuberculosis.Biopsies from 28 cases 19 controls with other diseases were subjected to including direct smears mycobacteria, culture both PCR....
Evidence link bacterial enterotoxins to apparent crypt-cell like cells (CCLCs), and Alpha Defensin 5 ( DEFA5 ) expansion in the colonic mucosa of Crohn’s colitis disease (CC) patients. These areas ectopic ileal metaplasia, positive for Paneth cell (PC) markers are consistent with diagnosis CC. Retrospectively, we: 1. Identified 21 patients indeterminate (IC) between 2000–2007 were reevaluation their final clinical 2014 after a followed-up mean 8.7±3.7 (range, 4–14) years. Their initial...
The parasitic protozoan Leishmania invades mammalian macrophages to establish infection. We reported previously that manipulates the expression of several non-coding RNA genes (e.g. Alu RNA, B1 and signal recognition particle RNA) in favor establishment their infection phagolysosomes these cells (Ueda, Y., Chaudhuri, G. (2000) J. Biol. Chem. 275, 19428-19432; Misra, S., Tripathi, M. K., (2005) 280, 29364-29373). report here mechanism this down-regulation. found (ncRNA) are repressed by...
Escherichia coli-bearing Dr-adhesins (Dr+ E. coli) cause chronic pyelonephritis in pregnant women and animal models. This renal infection correlates with the capacity of bacteria to invade epithelial cells expressing CD55. The mechanism remains unknown. CD55 amino acids vicinity binding pocket-Ser155 for Dr-adhesin were mutated alanine subjected temporal gentamicin-invasion/gentamicin-survival assay Chinese hamster ovary cells. CD55/microtubule (MT) responses studied using confocal/electron...
We reported previously that reduction in beta-arrestin 1 (β-AR 1) protein levels peripheral blood mononuclear leukocytes (PBMC) significantly correlated with the severity of depressive symptoms reproductive women. In this pilot study, we used β-AR PBMC as a marker for developing and Hamilton Depression Rating Scale (HAM-D) scores to assess potential mood-related side effects oral contraceptive use routine birth control among evaluated 29 women study. enrolled participants three groups:...
Two cases of congenital tuberculosis are described. Case 1 presented at 12 days age. The mother had been symptomatic for in the 1st trimester but was not diagnosed until her infant developed symptoms. infant's gastric aspirate acid-fast-bacilli (AFB)-positive and Mycobacterium culture-positive. PCR on specimen mother's sputum demonstrated identical strains. 2 45 age both AFB- asymptomatic contact-tracing family failed to detect infection. However, genital an endometrial biopsy.
COVID-19, caused by the highly transmissible severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has rapidly spread and become a pandemic since its outbreak in 2019. We have previously discovered that aloperine is new privileged scaffold can be modified to specific antiviral compound with markedly improved potency against different viruses, such as influenza virus. In this study, we identified collection of derivatives inhibit entry SARS-CoV-2 into host cells. Compound 5 most...
The protozoan parasite, Trypanosoma cruzi, causes severe morbidity and mortality in afflicted individuals. Approximately 30% of T. cruzi infected individuals present with cardiac pathology. invasive forms the parasite are carried vascular system to infect other cells body. During transportation, molecular mechanisms by which signals interact host endothelial (EC) especially heart endothelium is currently unknown. increases thrombospondin-1 (TSP1) expression activates Wnt/β-catenin hippo...
The protein translocase of the mitochondrial inner membrane in Trypanosoma brucei, TbTIM17, forms a modular complex association with several other trypanosome-specific proteins. To identify transiently interacting proximal partner(s) TbTim17, we used Biotinylation Identification (BioID) by expressing modified biotin ligase-TbTim17 (BirA∗-TbTim17) fusion T. brucei. BirA∗-TbTim17 was targeted to mitochondria and assembled TbTIM complex. In presence biotin, biotinylated Interestingly,...