A5101953064- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Plant tissue culture and regeneration
- Peptidase Inhibition and Analysis
- Plant Virus Research Studies
- Immune Cell Function and Interaction
- Plant Micronutrient Interactions and Effects
- HIV/AIDS Research and Interventions
- Cytomegalovirus and herpesvirus research
- RNA Research and Splicing
- Plant Stress Responses and Tolerance
- Signaling Pathways in Disease
- Plant-Microbe Interactions and Immunity
- RNA modifications and cancer
- Protease and Inhibitor Mechanisms
- Plant nutrient uptake and metabolism
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- Legume Nitrogen Fixing Symbiosis
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Agricultural pest management studies
- DNA and Nucleic Acid Chemistry
- GABA and Rice Research
- Transgenic Plants and Applications
Meharry Medical College
2015-2024
SRM Dental College
2018-2024
Chettinad Health City
2018-2023
Meenakshi Ammal Dental College and Hospital
2023
Lesbian, Gay, Bisexual and Transgender Community Center
2022
Creative Commons
2018
Ragas Dental College & Hospital
2018
Johns Hopkins University Center for AIDS Research
2018
National Cancer Institute
2018
Center for Cancer Research
2018
Though central to our understanding of how roots perform their vital function scavenging water and solutes from the soil, no direct genetic evidence currently exists support foundational model that suberin acts form a chemical barrier limiting extracellular, or apoplastic, transport in plant roots. Using newly characterized enhanced suberin1 (esb1) mutant, we established connection Arabidopsis thaliana between root both movement through solute accumulation shoot. Esb1 mutants, by increased...
Plants are sessile and therefore have developed mechanisms to adapt their environment, including the soil mineral nutrient composition. Ionomics is a developing functional genomic strategy designed rapidly identify genes gene networks involved in regulating how plants acquire accumulate these nutrients from soil. Here, we report on coupling of high-throughput elemental profiling shoot tissue various Arabidopsis accessions with DNA microarray-based bulk segregant analysis reverse genetics,...
Molybdenum (Mo) is an essential micronutrient for plants, serving as a cofactor enzymes involved in nitrate assimilation, sulfite detoxification, abscisic acid biosynthesis, and purine degradation. Here we show that natural variation shoot Mo content across 92 Arabidopsis thaliana accessions controlled by mitochondrially localized transporter (Molybdenum Transporter 1 - MOT1) belongs to the sulfate superfamily. A deletion MOT1 promoter strongly associated with low Mo, occurring seven of...
Summary Micro RNA s (mi s) are a major class of small non‐coding with emerging functions in biotic and abiotic interactions. Here, we report on new functional role Arabidopsis miR827 its NITROGEN LIMITATION ADAPTATION ( NLA ) target gene mediating plant susceptibility to the beet cyst nematode Heterodera schachtii . Cyst nematodes sedentary endoparasites that induce formation multinucleated feeding structures termed syncytia roots host plants. Using promoter: GUS fusion assays established...
Alfalfa mosaic virus (AMV) coat protein (CP) is essential for many steps in replication from early infection to encapsidation. However, the identity and functional relevance of cellular factors that interact with CP remain unknown. In an unbiased yeast two-hybrid screen CP-interacting Arabidopsis proteins, we identified several novel interactions could potentially modulate AMV replication. this report, focus on one CP-binding partners, PsbP protein, which a nuclear-encoded component...
Ferns (Pteridophyta) are very important members of the plant kingdom that lag behind other taxa with regards to our understanding their genetics, genomics, and molecular biology. We report here, knowledge, first instance stable transformation fern recovery transgenic sporophytes. Spores arsenic hyperaccumulating Pteris vittata tetraploid 'C-fern Express' (Ceratopteris thalictroides) were stably transformed by Agrobacterium tumefaciens constructs containing P. actin promoter driving a GUSPlus...
The HIV-1 capsid protein (CA) facilitates reverse transcription and nuclear entry of the virus. However, CA's role in post-nuclear steps remains speculative. We describe a direct link between CA integration by employing inhibitor PF74 as probe coupled with biochemical analysis preintegration complexes (PICs) isolated from acutely infected cells. At low micromolar concentration, potently inhibited infection without affecting transcription. Surprisingly, markedly reduced proviral owing to...
MiR-124 is a highly expressed miRNA in the brain and regulates genes involved neuronal function. We report that miR-124 post-transcriptionally PARP-1. have identified conserved binding site of 3'-untranslated region (3'UTR) Parp-1 mRNA. demonstrate directly binds to 3'UTR mutations seed sequences abrogate between two RNA molecules. Luciferase reporter assay revealed activity dopaminergic cell model. Interestingly, overlapped with target sequence miR-125b, another post-transcriptional...
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HIV-1 DNA is preferentially integrated into chromosomal hot spots by the preintegration complex (PIC). To understand mechanism, we measured integration activity of PICs-extracted from infected cells-and intasomes, biochemically assembled PIC substructures using a number relevant target substrates. We observed that PIC-mediated human chromatin preferred compared to genomic DNA. Surprisingly, nucleosomes lacking histone modifications were not analogous naked Nucleosomes containing...
MicroRNAs (miRNAs) are a class of small noncoding RNAs that post-transcriptionally regulate cellular gene expression. MiRNAs bind to the 3' untranslated region (UTR) target mRNA inhibit protein translation or in some instances cause degradation. The binding miRNA UTR is mediated by 2–8 nucleotide seed sequence at 5' end miRNA. While role miRNAs as regulatory molecules well established, identification mRNAs with functional relevance remains challenge. Bioinformatic tools have been employed...
Abstract Methamphetamine (METH) is a highly addictive psychostimulant that causes long-lasting effects in the brain and increases risk of developing neurodegenerative diseases. The cellular molecular METH are functionally linked to alterations glutamate levels. Despite well-documented on neurotransmission, underlying mechanism by which alters levels not clearly understood. In this study, we report an essential role proline biosynthesis maintaining METH-induced homeostasis. We observed acute...
Abstract Cocaine exposure alters gene expression in the brain via methylation and acetylation of histones along with DNA. Recently, poly (ADP-ribose) polymerase-1 (PARP-1) catalyzed PARylation has been reported as an important regulator cocaine-mediated expression. In this study, we report that cellular microRNA “miR-125b” plays a key role for cocaine-induced PARP-1 Acute chronic cocaine resulted downregulation miR-125b concurrent upregulation dopaminergic neuronal cells nucleus accumbens...