A5050417912- Phytoplasmas and Hemiptera pathogens
- Insect symbiosis and bacterial influences
- Studies on Chitinases and Chitosanases
- Insect Resistance and Genetics
- Insect-Plant Interactions and Control
- Cocoa and Sweet Potato Agronomy
- Invertebrate Immune Response Mechanisms
- Plant Reproductive Biology
- Neurobiology and Insect Physiology Research
- Plant Pathogens and Fungal Diseases
- Entomopathogenic Microorganisms in Pest Control
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Developmental Biology and Gene Regulation
- Plant-Microbe Interactions and Immunity
- Wheat and Barley Genetics and Pathology
- Plant biochemistry and biosynthesis
- Plant and Fungal Species Descriptions
- Genetic diversity and population structure
- Plant responses to water stress
- Plant Surface Properties and Treatments
- Powdery Mildew Fungal Diseases
- Postharvest Quality and Shelf Life Management
Ithaca College
2017-2024
Cornell University
2017-2024
Triangle
2024
Syngenta (United States)
2024
Indian River State College
2021
Kansas State University
2021
U.S. Horticultural Research Laboratory
2021
University of Cincinnati
2021
University of Arizona
2021
University of Aberdeen
2015
Ferns are the closest sister group to all seed plants, yet little is known about their genomes other than that they generally colossal. Here, we report on of Azolla filiculoides and Salvinia cucullata (Salviniales) present evidence for episodic whole-genome duplication in ferns-one at base 'core leptosporangiates' one specific Azolla. One fern-specific gene identified, recently shown confer high insect resistance, seems have been derived from bacteria through horizontal transfer. coexists a...
Though central to our understanding of how roots perform their vital function scavenging water and solutes from the soil, no direct genetic evidence currently exists support foundational model that suberin acts form a chemical barrier limiting extracellular, or apoplastic, transport in plant roots. Using newly characterized enhanced suberin1 (esb1) mutant, we established connection Arabidopsis thaliana between root both movement through solute accumulation shoot. Esb1 mutants, by increased...
The endodermis acts as a "second skin" in plant roots by providing the cellular control necessary for selective entry of water and solutes into vascular system. To enable such control, Casparian strips span cell wall adjacent endodermal cells to form tight junction that blocks extracellular diffusion across endodermis. This is composed lignin polymerized oxidative coupling monolignols through action NADPH oxidase peroxidases. strip domain proteins (CASPs) correctly position this biosynthetic...
The endodermis in roots acts as a selectivity filter for nutrient and water transport essential growth development. This is enabled by the formation of lignin-based Casparian strips. strip initiated localization domain proteins (CASPs) plasma membrane, at site where will form. Localized CASPs recruit Peroxidase 64 (PER64), Respiratory Burst Oxidase Homolog F, Enhanced Suberin 1 (ESB1), dirigent-like protein, to assemble lignin polymerization machinery. However, factors that control both...
Abstract The original Heinz 1706 reference genome was produced by a large team of scientists from across the globe variety input sources that included 454 sequences in addition to full-length BACs, BAC and fosmid ends sequenced with Sanger technology. We present here latest tomato (SL4.0) assembled de novo PacBio long reads scaffolded using Hi-C contact maps. assembly validated Bionano optical maps 10X linked-read sequences. This is highly contiguous fewer gaps compared previous builds...
Solanum pimpinellifolium (SP) is the wild progenitor of cultivated tomato. Because its remarkable stress tolerance and intense flavor, SP has been used as an important germplasm donor in modern tomato breeding. Here, we present a high-quality chromosome-scale genome sequence LA2093. Genome comparison identifies more than 92,000 structural variants (SVs) between LA2093 cultivar, Heinz 1706. Genotyping these SVs ~600 representative accessions alleles under selection during domestication,...
https://citrusgreening.org/.
Abstract Modern breeding methods integrate next-generation sequencing and phenomics to identify plants with the best characteristics greatest genetic merit for use as parents in subsequent cycles ultimately create improved cultivars able sustain high adoption rates by farmers. This data-driven approach hinges on strong foundations data management, quality control, analytics. Of crucial importance is a central database (1) track materials, (2) store experimental evaluations, (3) record...
High quality gene models are necessary to expand the molecular and genetic tools available for a target organism, but these only handful of model organisms that have undergone extensive curation experimental validation over course many years. The majority present in biological databases today been identified draft genome assemblies using automated annotation pipelines frequently based on orthologs from distantly related usually minor or major errors. Manual is time consuming often requires...
Abstract We have created an open access web portal with pathosystem-wide resources and bioinformatics tools for the host citrus, vector Asian citrus psyllid (ACP) multiple pathogens including Ca . Liberibacter asiaticus. To best of our knowledge, this is first example a database to use pathosystem as holistic framework understand insect transmitted plant disease. This endeavor integrates enables analysis data sets generated by community study greening disease complex. Users can submit...
Abstract Background Diaphorina citri is a vector of “ Candidatus Liberibacter asiaticus” ( C Las), the gram-negative bacterial pathogen associated with citrus greening disease. Control measures rely on pesticides negative impacts environment, natural ecosystems and human animal health. In contrast, gene-targeting methods have potential to specifically target species and/or reduce transmission. Results To improve genomic resources needed for targeted pest control, we assembled D. genome based...
Lasiodiplodia theobromae is a fungal pathogen associated with perennial tropical fruit plants worldwide. In citrus, L. causes stem-end rot (Diplodia rot), damaging postharvest disease that aggravated when trees are also infected the citrus greening bacteria ‘Candidatus Liberibacter asiaticus’. Due to latent infection of during preharvest stage, it becomes difficult control by chemical or physical treatment. current study, we sequenced and assembled strain CITRA15, first genome obtained from...
The polysaccharide chitin is critical for the formation of many insect structures, including exoskeleton, and required normal development. Here we report annotation three genes from synthesis pathway in Asian citrus psyllid, Diaphorina citri (Hemiptera: Liviidae), vector Huanglongbing (citrus greening disease). Most insects have two synthase (CHS) but, like other hemipterans, D. has only one. In contrast, unusual among having UDP-N-acetylglucosamine pyrophosphorylase (UAP) genes. One UAP...
ABSTRACT The Asian citrus psyllid ( Diaphorina citri Kuwayama) is the insect vector of bacterium Candidatus Liberibacter asiaticus (CLas), pathogen associated with Huanglongbing (HLB, greening). HLB threatens production worldwide. Suppression or reduction using chemical insecticides has been primary method to inhibit spread greening disease. Accurate structural and functional annotation genome, as well a clear understanding interactions between CLas, are required for development new...
The Asian citrus psyllid, Diaphorina citri, is an insect vector that transmits Candidatus Liberibacter asiaticus, the causal agent of Huanglongbing (HLB), or greening disease. This disease has devastated Florida’s industry, and threatens California’s industry as well other producing regions around world. To find novel solutions to disease, a better understanding needed. D. citri genome been used identify characterize genes involved in Wnt signaling pathways. utilized for many important...
Huanglongbing (HLB), also known as citrus greening disease, is caused by the bacterium Candidatus Liberibacter asiaticus (CLas). It a serious threat to global production. This transmitted Asian psyllid, Diaphorina citri (Hemiptera). There are no effective in planta treatments for CLas. Therefore, one strategy manage psyllid population. Manual annotation of D. genome can identify and characterize gene families that could be novel targets control. The yellow family an excellent target because...
Chitin deacetylases (CDAs) are one of the least understood components insect chitin metabolism. The partial deacetylation polymers appears to be important for proper formation higher order structures, such as long fibers and bundles, which contribute integrity exoskeleton other structures. Some CDAs may also involved in bacterial defense. Here, we report manual annotation four CDA genes from Asian citrus psyllid, Diaphorina citri, laying groundwork future study these genes.
Chitinases are enzymes that digest the polysaccharide polymer chitin. During insect development, breakdown of chitin is an essential step in molting exoskeleton. Knockdown chitinases required for lethal to insects, making chitinase genes interesting target RNAi-based pest control methods. The Asian citrus psyllid, Diaphorina citri, carries bacterium causing Huanglongbing, or greening disease, a devastating disease. We identified and annotated 12 family from D. citri as part community effort...
Hox genes and their cofactors are essential developmental specifying regional identity in animals. have a conserved arrangement clusters the same order which they specify along anterior–posterior axis. A few insect species breaks cluster, but these exceptions. We annotated 10 of Asian citrus psyllid Diaphorina citri, found split its cluster between Deformed Sex combs reduced – first time break at this position has been observed an cluster. also D. citri orthologs cofactor homothorax, PKNOX...
Insects have a segmented body plan that is established during embryogenesis when the anterior–posterior (A–P) axis divided into repeated units by cascade of gene expression. The initiated protein gradients created translation maternally provided mRNAs, localized at anterior and posterior poles embryo. Combinations these proteins activate specific gap genes to divide embryo distinct regions along axis. Gap then pair-rule genes, which are usually expressed in parts every other segment. turn,...
Citrus greening disease is caused by the pathogen Candidatus Liberibacter asiaticus and transmitted Asian citrus psyllid, Diaphorina citri. No curative treatment or significant prevention mechanism exists for this disease, which causes economic losses from reduced production. A high-quality genome of D. citri being manually annotated to provide accurate gene models identify novel control targets increase understanding pest. Here, we 25 genes involved in glycolysis gluconeogenesis, seven...
Abstract Chitin deacetylases (CDAs) are one of the least understood components insect chitin metabolism. The partial deacetylation polymers appears to be important for proper formation higher order structures, such as long fibers and bundles, that contribute integrity exoskeleton other structures. Some CDAs may also play a role in bacterial defense. Here we report characterization four CDA genes from Asian citrus psyllid, Diaphorina citri, laying groundwork future study these genes. D. citri...
Abstract The Asian citrus psyllid, Diaphorina citri , is an insect vector that transmits Candidatus Liberibacter asiaticus, the causal agent of Huanglongbing (HLB) or greening disease. This disease has devastated Florida’s industry and threatens California’s as well other producing regions around world. To find novel solutions to disease, a better understanding needed. D. genome been used identify characterize genes involved in Wnt signaling pathways. utilized for many important biological...