A5064164775- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Advanced MEMS and NEMS Technologies
- Force Microscopy Techniques and Applications
- Adhesion, Friction, and Surface Interactions
- Single-cell and spatial transcriptomics
- Quantum, superfluid, helium dynamics
- Mechanical and Optical Resonators
- Cell Image Analysis Techniques
- CAR-T cell therapy research
- Advanced Fluorescence Microscopy Techniques
- Atomic and Subatomic Physics Research
- Metal and Thin Film Mechanics
- Cancer Genomics and Diagnostics
- Spacecraft and Cryogenic Technologies
- Diamond and Carbon-based Materials Research
- Advanced machining processes and optimization
- Microfluidic and Bio-sensing Technologies
- Advanced Biosensing Techniques and Applications
- Health, Environment, Cognitive Aging
- Advanced Sensor and Energy Harvesting Materials
- Cancer Cells and Metastasis
- Cold Atom Physics and Bose-Einstein Condensates
- Methane Hydrates and Related Phenomena
GE Global Research (United States)
2012-2024
Core Research Center
2022
General Electric (United States)
2008-2013
Sandia National Laboratories
2004-2010
Sandia National Laboratories California
2005-2009
National Technical Information Service
2005-2006
Office of Scientific and Technical Information
2005-2006
Purdue University West Lafayette
2006
Material (Belgium)
2005
Silicon Technologies (United States)
2005
Limitations on the number of unique protein and DNA molecules that can be characterized microscopically in a single tissue specimen impede advances understanding biological basis health disease. Here we present multiplexed fluorescence microscopy method (MxIF) for quantitative, single-cell, subcellular characterization multiple analytes formalin-fixed paraffin-embedded tissue. Chemical inactivation fluorescent dyes after each image acquisition round allows reuse common iterative staining...
A simple image-based autofocusing scheme for digital microscopy is demonstrated that uses as few two intermediate images to bring the sample into focus. The algorithm adapted a commercial inverted microscope and used automate brightfield fluorescence imaging of histopathology tissue sections.
This work demonstrates a polycrystalline silicon surface-micromachined inchworm actuator that exhibits high-performance characteristics such as large force (/spl plusmn/0.5 millinewtons), velocity range (0 to /spl plusmn/4.4 mm/sec), displacement plusmn/100 microns), small step size plusmn/10, plusmn/40 or nanometers), low power consumption (nanojoules per cycle), continuous bidirectional operation and relatively area (600 times/ 200/spl mu/m/sup 2/). An in situ load spring calibrated on...
Epithelial organ morphogenesis involves reciprocal interactions between epithelial and mesenchymal cell types to balance progenitor retention expansion with differentiation for evolution of tissue architecture. Underlying submandibular salivary gland branching is the regulated proliferation perhaps several populations, which have not been characterized throughout development, yet are critical understanding regeneration, disease. Here we applied a serial multiplexed fluorescent...
Mapping the human body at single cell resolution in three dimensions (3D) is important for understanding cellular interactions context of tissue and organ organization. 2D spatial analysis a section may be limited by numbers histology. Here we show workflow 3D reconstruction multiplexed sequential sections: MATRICS-A (Multiplexed Image Three-D Reconstruction Integrated Cell Spatial - Analysis). We demonstrate 26 serial sections fixed skin (stained with 18 biomarkers) from 12 donors aged...
We measure friction of monolayer-lubricated microelectromechanical systems surfaces under both static and dynamic conditions while continuously controlling the applied normal load at positive or negative values (i.e., compression tension). The experiment methodology we have devised enables fitting to complete one-dimensional equation motion. observe zero load, quantitatively attribute this interfacial adhesion. Within error, adhesive force is same conditions.
We describe the design and operation of a long-working-distance, incoherent light interference microscope that has been developed to address growing demand for new microsystem characterization tools. The is similar Linnik thus preserves full working distance long-working-distance objectives utilized. However, in contrast traditional microscope, does not rely on use matched sample reference arms interferometer. An adjustable optical configuration devised allows total path length, wavefront...
Multiplexed immunofluorescence is a powerful tool for validating multigene assays and understanding the complex interplay of proteins implicated in breast cancer within morphological context. We describe novel technology imaging an extended panel biomarkers on single, formalin-fixed paraffin-embedded sample evaluating biomarker interaction at single-cell level, demonstrate proof-of-concept small set tumours, including those which co-express hormone receptors with Her2/neu Ki-67.Using...
Abstract Cancer cells’ ability to inhibit apoptosis is key malignant transformation and limits response therapy. Here, we performed multiplexed immunofluorescence analysis on tissue microarrays with 373 cores from 168 patients, segmentation of 2.4 million individual cells, quantification 18 cell lineage proteins. We identified an enrichment for BCL2 in immune, BAK, SMAC, XIAP cancer cells. Ordinary differential equation-based modeling sensitivity at single-cell resolution was conducted atlas...
Multiplexed immunofluorescence bioimaging of single-cells and their spatial organization in tissue holds great promise to the development future precision diagnostics therapeutics. Current multiplexing pipelines typically involve multiple rounds staining across slides. This introduces experimental batch effects that can hide underlying biological signal. It is important have robust algorithms correct for while not introducing biases into data. Performance data normalization methods vary...
Colorectal cancer (CRC) has one of the highest incidences and mortality rates. In stage III, postoperative chemotherapy benefits <20% patients, while more than 50% will develop distant metastases. Biomarkers for identification patients at increased risk disease recurrence following adjuvant are currently lacking. this study, we assessed immune signatures in tumor microenvironment (TME) using an situ multiplexed immunofluorescence imaging single-cell analysis technology (Cell DIVETM)...
Particulates can strongly influence interfacial adhesion between rough surfaces by changing their average separation. In a cantilever beam test structure, compressive zone exists just beyond the crack tip, which may act to deform such particles. To explore this phenomenon quantitatively, we compared finite element method calculations of interface load-displacement experiments individual Below certain threshold density, show that stress distribution at is sufficient regime, controlled...
The authors describe a micromachined force sensor that is able to measure forces as small 1pN in both air and water. First, they measured the field produced by an electromagnet on individual 2.8μm magnetic beads glued sensor. By repeating with 11 different beads, 9% standard deviation saturation magnetization. They next demonstrated was fully functional when immersed physiological buffer. These results show sensors can be useful for calibration also measurement of biophysical chip.
Using a high-performance polycrystalline-silicon micromachined actuator that also functions as friction tester, we have found frictional forces cannot be explained by Amontons' law with constant coefficient of friction. In our test, tangential force is applied while normal load ramped down at rate \mathdot <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">F</i> <sub xmlns:xlink="http://www.w3.org/1999/xlink">n</sub> after hold time...
Recently advances in miniaturization and automation have been utilized to rapidly decrease the time result for microbiology testing clinic. These made due limitations of conventional culture-based methods, including agar plate microbroth dilution, which long turnaround times require physicians treat patients empirically with antibiotics before test results are available. Currently, there exist similar pharmaceutical sterility bioburden testing, where associated standard drive costly...
Measurements on monolayer-coated polycrystalline silicon surfaces have shown that the static friction coefficient ${\ensuremath{\mu}}_{s}$ strongly depends loading parameters including hold time and normal force by Corwin de Boer [J. Microelectromech. Syst. 18, 250 (2009)]. In work, was measured keeping tangential constant lowering until motion occurred. Results indicated also ramp-down rate. Here, we postulate if load is lowered instantaneously, for block to begin moving, ``release time''...
We present a mesodissection platform that retains the advantages of laser-based dissection instrumentation with speed and ease manual dissection. Tissue in clinical laboratories is often performed by manually scraping physician-selected region from standard glass slide mounts. In this manner, costs associated remain low, but spatial resolution compromised. contrast, laser microdissection methods maintain matches requirements for analysis important tissue heterogeneity remains costly labor...
Abstract Mapping the human body at single cell resolution in three-dimensions (3D) is an important step toward a “digital twin” model that captures structure and dynamics of cell-cell interactions. Current 3D imaging methods suffer from low are limited their ability to distinguish types spatial relationships. We present novel workflow: MATRICS-A ( M ultiplexed Im ge T hree-D R econstruction I ntegrated C ell S patial - A nalysis) generates map cells multiplexed images calculates type...
Friction and wear are extremely important issues in micromachined surfaces design applications that allow rubbing. A polysilicon surface-micromachined inchworm device has been developed to obtain detailed in-situ information on these properties under well-controlled loading conditions. Here, we investigate the operational evolution of friction coefficient as a function number imposed cycles. test procedure was monitor various functional parameters such travel distance an drag force for fixed...