A5087618896- Salivary Gland Disorders and Functions
- Proteoglycans and glycosaminoglycans research
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Axon Guidance and Neuronal Signaling
- Electrospun Nanofibers in Biomedical Applications
- Mesenchymal stem cell research
- dental development and anomalies
- Cell Adhesion Molecules Research
- Cancer Cells and Metastasis
- Fibroblast Growth Factor Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Neonatal Respiratory Health Research
- Salivary Gland Tumors Diagnosis and Treatment
- Hippo pathway signaling and YAP/TAZ
- Wnt/β-catenin signaling in development and cancer
- Prostate Cancer Treatment and Research
- Developmental Biology and Gene Regulation
- Nerve injury and regeneration
- Connective tissue disorders research
- Single-cell and spatial transcriptomics
- Prostate Cancer Diagnosis and Treatment
- Spectroscopy and Chemometric Analyses
- Hormonal and reproductive studies
Albany State University
2015-2025
University at Albany, State University of New York
2016-2025
New York University
2019-2022
SUNY Polytechnic Institute
2022
National Institute of Dental and Craniofacial Research
1975-2011
National Institutes of Health
1975-2011
Rensselaer Polytechnic Institute
2010
GE Global Research (United States)
2007
General Electric (Norway)
2007
Baylor College of Medicine
1995-2003
Limitations on the number of unique protein and DNA molecules that can be characterized microscopically in a single tissue specimen impede advances understanding biological basis health disease. Here we present multiplexed fluorescence microscopy method (MxIF) for quantitative, single-cell, subcellular characterization multiple analytes formalin-fixed paraffin-embedded tissue. Chemical inactivation fluorescent dyes after each image acquisition round allows reuse common iterative staining...
Branching morphogenesis of mouse submandibular glands is regulated by multiple growth factors. Here, we report that ex vivo branching intact decreases when either FGFR2 expression downregulated or soluble recombinant FGFR2b competes out the endogenous However, a combination neutralizing antibodies to FGF1, FGF7 and FGF10 required inhibit in gland, suggesting FGF isoforms are for branching. Exogenous FGFs added epithelial rudiments cultured without mesenchyme induce distinct morphologies....
Analyses of gene expression profiles at five different stages mouse submandibular salivary gland development provide insight into organogenesis and identify genes that may be critical stages. Genes with similar were clustered, RT-PCR was used to confirm the developmental changes. We focused on fibroblast growth factor receptor 1 (FGFR1), as its is highest early in development. extended our array results analyzed patterns other FGFR FGF isoforms. The functional significance FGFR1 confirmed by...
Branching morphogenesis is a dynamic developmental process shared by many organs, but the mechanisms that reorganize cells during branching are not well understood. We hypothesized extensive cell rearrangements involved, and investigated migration using two-color confocal time-lapse microscopy to image extracellular-matrix dynamics in developing salivary glands. labeled submandibular gland (SMG) epithelial with green fluorescent protein matrix fibronectin. Surprisingly, we observed...
Embryonic tissues may provide clues about mechanisms required for tissue reassembly and regeneration, but few studies have utilized primary embryonic to study assembly. To test the capacity of fragments regenerate, we cultured day 13 (E13) mouse submandibular gland (SMG) epithelium found that as small a quarter-bud retain ability branch. Further, completely dissociated SMG epithelial cells self-organize into structures undergo significant branching. Investigation involved in self-assembly...
Abstract Sjögren's disease is an autoimmune disorder affecting exocrine glands, causing dry eyes and mouth other morbidities. Polypharmacy or a history of radiation to the head neck can also lead mouth. Sjogren's often underdiagnosed due its non-specific symptoms, limited awareness among healthcare professionals, complexity diagnostic criteria, limiting ability provide therapy early. Current methods suffer from limitations including variation in individuals, absence single marker, low...
Aquaporin 5 (AQP5) is known to be central for salivary fluid secretion. A study of the temporal-spatial distribution AQP5 during submandibular gland (SMG) development and in adult tissues might offer further clues its unknown role development. In present work, SMGs from embryonic day (E) 14.5–18.5 postnatal days (P) 0, 2, 5, 25, 60 were immunostained analyzed using light microscopy. Additional confocal transmission electron microscopy performed on P60 glands. Our results show that expression...
Epithelial organ morphogenesis involves reciprocal interactions between epithelial and mesenchymal cell types to balance progenitor retention expansion with differentiation for evolution of tissue architecture. Underlying submandibular salivary gland branching is the regulated proliferation perhaps several populations, which have not been characterized throughout development, yet are critical understanding regeneration, disease. Here we applied a serial multiplexed fluorescent...
The basement membrane is crucial for epithelial tissue organization and function. However, the mechanisms by which restricted to basal periphery of tissues membrane-mediated signals that regulate coordinated are not well defined. Here, we report Rho kinase (ROCK) controls restricting in developing mouse submandibular salivary glands, ROCK inhibition results accumulation ectopic throughout compartment. ROCK-regulated restriction PAR-1b (MARK2) localization outer cell layer required...
ABSTRACT Epithelial progenitor cells are dependent upon a complex 3D niche to promote their proliferation and differentiation during development, which can be recapitulated in organoids. The specific requirements of the remain unclear for many cell types, including proacinar that give rise secretory acinar epithelial produce saliva. Here, using ex vivo cultures E16 primary mouse submandibular salivary gland clusters, we investigated requirement mesenchymal other factors producing organoids...
Fibrosis results from excess extracellular matrix accumulation, which alters normal tissue architecture and impedes function. In the salivary gland, fibrosis can be induced by irradiation treatment for cancer therapy, Sjögren's Disease, other causes; however, it is unclear stromal cells signals participate in injury responses disease progression. As hedgehog signaling has been implicated of gland organs, we examined contributions effector, Gli1, to fibrotic glands. To experimentally induce a...
Salivary gland hypofunction is highly prevalent in aged and diseased individuals leading to significant discomfort morbidity. One factor that contributes salivary cellular aging, or senescence. Senescent cells can impair function by secreting paracrine-acting growth factors cytokines, known as senescence-associated secretory phenotype (SASP) factors. These SASP stimulate inflammation, propagate the senescent through bystander effect, fibrosis. As senotherapeutics target have shown...
We have reported previously that syndecan-1 (Sdc1)-null mice show delayed re-epithelialization after skin and corneal wounding. Here, we primary keratinocytes obtained from Sdc1-null grown for 3-5 days in culture are more proliferative, adherent migrate slowly than wt keratinocytes. However, the migration rates of can be restored to wild-type levels by replating onto tissue plates coated with fibronectin collagen I, laminin (LN)-332 or matrices produced cells. Migration also treating...