A5018204546- Vagus Nerve Stimulation Research
- Neuroscience and Neural Engineering
- Heart Rate Variability and Autonomic Control
- EEG and Brain-Computer Interfaces
- Nerve injury and regeneration
- Nicotinic Acetylcholine Receptors Study
- Neurological disorders and treatments
- Ultrasound and Hyperthermia Applications
- Genetic Neurodegenerative Diseases
- Pain Management and Treatment
- Pluripotent Stem Cells Research
- Photoreceptor and optogenetics research
- Cardiac Arrhythmias and Treatments
- Cardiovascular Syncope and Autonomic Disorders
- Spaceflight effects on biology
- Attention Deficit Hyperactivity Disorder
- Mitochondrial Function and Pathology
- Anesthesia and Sedative Agents
- Botulinum Toxin and Related Neurological Disorders
- Neuroscience and Neuropharmacology Research
- Non-Invasive Vital Sign Monitoring
- Transcranial Magnetic Stimulation Studies
- Treatment of Major Depression
- Neuroinflammation and Neurodegeneration Mechanisms
- Laser Applications in Dentistry and Medicine
Feinstein Institute for Medical Research
2020-2024
Northwell Health
2021-2024
Division of Undergraduate Education
2024
Marquette University
2016-2019
Inflammation is pathogenically implicated in pulmonary arterial hypertension; however, it has not been adequately targeted therapeutically. We investigated whether neuromodulation of an anti-inflammatory neuroimmune pathway involving the splenic nerve using noninvasive, focused ultrasound stimulation spleen (sFUS) can improve experimental hypertension.
Abstract Bioelectronic therapies modulating the vagus nerve are promising for cardiovascular, inflammatory, and mental disorders. Clinical applications however limited by side-effects such as breathing obstruction headache caused non-specific stimulation. To design selective functional stimulation, we engineered VaStim, a realistic efficient in-silico model. We developed protocol to personalize VaStim in-vivo using simple muscle responses, successfully reproducing experimental observations,...
Vagus nerve stimulation (VNS) suppresses inflammation and autoimmune diseases in preclinical clinical studies. The underlying molecular, neurological, anatomical mechanisms have been well characterized using acute electrophysiological of the vagus. However, there are several unanswered mechanistic questions about effects chronic VNS, which require solving numerous technical challenges for a long-term interface with vagus mice. Here, we describe scalable model VNS mice developed validated...
To restore function after injury to the CNS, axons must be stimulated extend into denervated territory and, critically, form functional synapses with appropriate targets. We showed previously that forced overexpression of transcription factor Sox11 increases axon growth by corticospinal tract (CST) neurons spinal injury. However, behavioral outcomes were not improved, raising question whether newly sprouted are able synapses. Here we developed an optogenetic strategy, paired single-unit...
Interfaces between the nervous and immune systems have been shown essential for coordination regulation of responses. Non-invasive ultrasound stimulation targeted to spleen has recently capable activating one such interface, splenic cholinergic anti-inflammatory pathway (CAP). Over past decade, CAP other neuroimmune pathways activated using implanted nerve stimulators tested prevent cytokine release inflammation. However, studies typically performed in models severe, systemic (e.g.,...
ObjectivesLow-intensity, focused ultrasound (FUS) is an emerging noninvasive neuromodulation approach, with improved spatial and temporal resolution penetration depth compared to other electrical stimulation strategies. FUS has been used modulate circuits in the brain peripheral nervous system, however, its potential spinal unclear. In this study, we assessed effect of trans-spinal (tsFUS) on reflexes healthy rats.Materials MethodstsFUS targeting different segments was delivered for 1...
Afferent and efferent fibers in the vagus nerve travel inside fascicles form branches to innervate organs regulate organ functions. The spatial organization of fascicles, with respect functions they mediate innervate, is unknown. Accordingly, it unknown whether such can be leveraged by bioelectronic devices for function- organ-specific neurostimulation. To characterize functional microscopic anatomy nerve, we developed a pipeline consisting micro-computed tomography 3D reconstruction...
Injury to the spinal cord often disrupts long-distance axon tracts that link brain and cord, causing permanent disability. Axon regeneration is then prevented by a combination of inhibitory signals emerge at injury site low capacity for within injured neurons. The corticospinal tract (CST) essential fine motor control but has proven refractory many attempted pro-regenerative treatments. Although strategies are emerging create relay or detour circuits re-route cortical commands through spared...
Abstract Vagal fibers travel inside fascicles and form branches to innervate organs regulate organ functions. Vagus nerve stimulation (VNS) therapies activate non-selectively, often resulting in reduced efficacy side effects from non-targeted organs. Transverse longitudinal arrangement of according functions they mediate is unknown, however it crucial for selective VNS. Using microcomputed tomography, we found that, swine, are arranged 2 overlapping axes, with sensory motor separated...
Abstract Vagus nerve stimulation (VNS) is a neuromodulation therapy with the potential to treat wide range of chronic conditions in which inflammation implicated, including type 2 diabetes, obesity, atherosclerosis and heart failure. Many these diseases have well-established mouse models but due significant surgical engineering challenges that accompany reliable interface for long-term VNS mice, therapeutic implications this bioelectronic approach remain unexplored. Here, we describe implant...
Quantitative descriptions of the morphology and structure peripheral nerves is central in development bioelectronic devices interfacing nerves. While histological procedures microscopy techniques yield high-resolution detailed images individual axons, automated methods to extract relevant information at single-axon level are not widely available. We implemented a segmentation algorithm that allows for subsequent feature extraction immunohistochemistry (IHC) single fiber scale. These features...
Cervical vagus nerve stimulation (VNS) is a neuromodulation therapy used in the treatment of several chronic disorders. In order to maximize therapeutic effectiveness VNS, it has become increasingly important deliver fiber-specific neurostimulation, so that undesired effects can be minimized. Assessing activation different vagal fiber types through electrical therefore essential for developing fiber-selective VNS therapies. Towards this goal, we conducted silico investigations using generic...
<title>Abstract</title> Vagus nerve stimulation (VNS) is emerging as potential treatment for several chronic diseases, however, limited control of fiber activation to promote desired effects over side restricts clinical translation. Here we describe a new VNS method that relies on intermittent, interferential sinusoidal current (i<sup>2</sup>CS) through implanted, multi-contact epineural cuffs. In swine, i<sup>2</sup>CS elicits specific potentials and end organ responses, distinct from...
Abstract Vagus nerve stimulation (VNS) is emerging as potential treatment for several chronic diseases, however, limited control of fiber activation to promote desired effects over side restricts clinical translation. Here we describe a new VNS method that relies on intermittent, interferential sinusoidal current (i 2 CS) through implanted, multi-contact epineural cuffs. In swine, i CS elicits specific potentials and end organ responses, distinct from equivalent non-interferential...
Abstract Background Inflammation is pathogenically implicated in pulmonary arterial hypertension (PAH); however, it has not been adequately targeted therapeutically. We investigated whether neuromodulation of an anti-inflammatory neuroimmune pathway involving the splenic nerve using noninvasive, focused ultrasound stimulation spleen (sFUS) can improve experimental (PH). Methods PH was induced rats either by SU5416 (20 mg/kg SQ) injection, followed 21 (or 35) days hypoxia (SuHx model), or...
Abstract Background The noradrenergic innervation of the spleen is implicated in autonomic control inflammation and has been target neurostimulation therapies for inflammatory diseases. However, there no real-time marker its successful activation, which hinders development anti-inflammatory mechanistic studies neural circuits. Methods In mice, we performed fast-scan cyclic voltammetry (FSCV) during intravenous injections norepinephrine (NE), stimulation vagus, splanchnic, or splenic nerves....