A5070712497- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Capillary Electrophoresis Applications
- Electrowetting and Microfluidic Technologies
- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Genetics, Aging, and Longevity in Model Organisms
- Lipid Membrane Structure and Behavior
- Electrohydrodynamics and Fluid Dynamics
- Lipid metabolism and biosynthesis
- Micro and Nano Robotics
- Spaceflight effects on biology
- Cystic Fibrosis Research Advances
- Nanomaterials and Printing Technologies
- thermodynamics and calorimetric analyses
- Erythrocyte Function and Pathophysiology
- Endoplasmic Reticulum Stress and Disease
- Field-Flow Fractionation Techniques
- Neuroscience and Neural Engineering
- Advanced Sensor and Energy Harvesting Materials
- CRISPR and Genetic Engineering
- Digital Holography and Microscopy
- Advanced Optical Sensing Technologies
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced Nanomaterials in Catalysis
- Surface Modification and Superhydrophobicity
KU Leuven
2021-2025
Pakistan Institute of Engineering and Applied Sciences
2022
ETH Zurich
2013-2021
Imperial College London
2010-2016
Bioengineering (Switzerland)
2014
Kensington Health
2011
Samueli Institute
2009
University of California, Irvine
2009
We report recent advances in the field of droplet-based microfluidics. Specifically, we highlight unique features such platforms for high-throughput experimentation; describe functional components that afford complex analytical processing and on applications synthesis, screening, cell biology synthetic systems biology. Issues including integration high-information content detection methods, long term droplet stability opportunities large scale intelligent biological experimentation are also...
Prime editing is a recent, CRISPR-derived genome technology capable of introducing precise nucleotide substitutions, insertions, and deletions. Here, we present prime approaches to correct L227R- N1303K-CFTR, two mutations that cause cystic fibrosis are not eligible for current market-approved modulator therapies. We show that, upon DNA correction the CFTR gene, complex glycosylation, localization, and, most importantly, function protein restored in HEK293T 16HBE cell lines. These findings...
Compartmentalised structures based on droplet interface bilayers (DIBs), including multisomes and compartmentalised vesicles, are seen by many as the next generation of biomimetic soft matter devices. Herein, we outline a microfluidic approach for construction miniaturised pL volumes in high-throughput demonstrate their potential vehicles situ chemical synthesis.
High-throughput screening and enrichment of antibody-producing cells have many important applications. Herein, we present a droplet microfluidic approach for high-throughput sorting antibody-secreting using Förster resonance electron transfer (FRET)-based assay. The FRET signal is mediated by the specific binding secreted antibody to two fluorescently labeled probes supplied within droplet. Functional hybridoma expressing either membrane-bound or monoclonal antibodies (mAbs), both, were...
Herein, we assess the functionality of magnetic helical microswimmers as basic tools for manipulation soft materials, including microdroplets and single cells. Their ability to perform a range unit operations is evaluated operational challenges associated with their use are established. In addition, also report on interactions observed between head such swimmers boundaries droplets cells discuss possibilities assembling an artificial swimming microorganism or motorized cell.
Microdroplets generated in microfluidic channels hold great promise for use as substrates high-throughput chemical and biological analysis. These water-in-oil compartments can serve isolated reaction vessels, since they be at rates excess of 1 kHz, thousands assays carried out quickly reproducibly. Nevertheless, sampling the large amount information from these platforms still remains a significant challenge. For example, considering high droplet generation velocities, reproducibility...
We present a passive microfluidic strategy for sorting adult C. elegans nematodes on the basis of age and size. The separation mechanism takes advantage phenotypic differences between 'adult' 'juvenile' organisms their behaviour in architectures. In brief, device allows worms to sort themselves manner.
We present the use of microfluidic "V-junctions" as a droplet generation strategy that incorporates enhanced performance characteristics when compared to more traditional "T-junction" formats. This includes ability generate target-sized droplets from very first one, efficient switching between multiple input samples, production wide range sizes (and size gradients) and facile with residence time gradients. Additionally, V-junction generators enables suspension subsequent resumption flows at...
Microfluidics enables the interference free observation of sensitive developmental processes in<italic>C. elegans</italic>.
We present a simple technique for the concentration of liquid samples in microfluidic devices applicable single or multiple-phase configurations. The strategy consists capturing sample interest within traps and breaking its continuity by introduction gas phase, which is also used to evaporate it.
A surface modification method for bonded polydimethylsiloxane (PDMS) microchannels is presented herein. Polymerization of acrylic acid was performed on the a microchannel using an inline atmospheric pressure dielectric barrier microplasma technique. The treatment changes wettability from hydrophobic to hydrophilic. This challenging task due fast recovery PDMS after modification. allows formation highly monodisperse oil-in-water (O/W) droplets. generation water-in-oil-in-water (W/O/W) double...
Abstract Significant non‐genetic stochastic factors affect aging, causing lifespan differences among individuals, even those sharing the same genetic and environmental background. In Caenorhabditis elegans , in heat‐shock response (HSR) are predictive of lifespan. However, contributing to heterogeneity HSR still not fully elucidated. Here, authors characterized dynamics isogenic C. expressing GFP reporter for hsp‐16.2 identifying key contributors heterogeneity. Specifically, microfluidic...
The development of microfluidic platforms for performing chemistry and biology has in large part been driven by a range potential benefits that accompany system miniaturisation. Advantages include the ability to efficiently process nano- femoto- liter volumes sample, facile integration functional components, an intrinsic predisposition towards large-scale multiplexing, enhanced analytical throughput, improved control reduced instrumental footprints.1 In recent years much interest focussed on...
Abstract In the quest to produce artificial cells, one key challenge that remains be solved is recreation of a complex cellular membrane. Among existing models, giant unilamellar vesicles (GUVs) are particularly interesting due their intrinsic compartmentalisation ability and resemblance in size shape eukaryotic cells. Many techniques have been developed GUVs all having inherent advantages disadvantages. Here, authors show fluorinated silica nanoparticles (FNPs) used form Pickering emulsions...
Polypyrrole (PPy)-based microactuators hold a promise for wide variety of engineering applications from robotics and microassembly to biosensors drug delivery systems. The main advantages using PPy/Au actuator structures (vs competing solid-state technologies) include ease fabrication, low actuation energy, large motion range microactuators. We present advances in two areas application - the extended-life biosensor platform micromixers.
Herein, a method is reported that combines droplet‐based microfluidics and microscope projection photolithography for the generation of poly ethylene glycol diacrylate microgels their encapsulation within pL‐volume droplets. By implementing continuous‐flow in vicinity cross junction, real‐time situ fiber‐like structures aqueous microdroplets demonstrated. The effect UV excitation assessed at varying distances from junction both constant pulsed modes, as route to controlling microfiber...