A5041162737- Liver Disease Diagnosis and Treatment
- Diet, Metabolism, and Disease
- Dietary Effects on Health
- Genomics, phytochemicals, and oxidative stress
- Endoplasmic Reticulum Stress and Disease
- RNA modifications and cancer
- Folate and B Vitamins Research
- Liver Disease and Transplantation
- RNA Research and Splicing
National University of Singapore
2021-2025
Duke-NUS Medical School
2022
Several recent clinical studies have shown that serum homocysteine (Hcy) levels are positively correlated, while vitamin B12 (B12) and folate negative with non-alcoholic steatohepatitis (NASH) severity. However, it is not known whether hyperhomocysteinemia (HHcy) plays a pathogenic role in NASH.We examined the effects of HHcy on NASH progression, metabolism, autophagy dietary genetic mouse models, patients, primates. We employed (Fol) to reverse features mice cell culture.Serum Hcy...
Protein translation is an energy-intensive ribosome-driven process that reduced during nutrient scarcity to conserve cellular resources. During prolonged starvation, cells selectively translate specific proteins enhance their survival (adaptive translation); however, this poorly understood. Accordingly, we analyzed protein and mRNA transcription by multiple methods in vitro vivo investigate adaptive hepatic starvation. While acute starvation suppressed general, proteomic analysis showed...
Protein translation is an energy-intensive ribosome-driven process that reduced during nutrient scarcity to conserve cellular resources. During prolonged starvation, cells selectively translate specific proteins enhance their survival (adaptive translation); however, this poorly understood. Accordingly, we analyzed protein and mRNA transcription by multiple methods in vitro vivo investigate adaptive hepatic starvation. While acute starvation suppressed general, proteomic analysis showed...
Currently, little is known about the mechanism(s) regulating global and specific protein translation during metabolic dysfunction-associated steatohepatitis (MASH; previously as non-alcoholic steatohepatitis, NASH).
Abstract Background Currently, little is known about the mechanism(s) regulating global and specific protein translation during non-alcoholic steatohepatitis (NASH). Methods We used puromycin-labelling, polysome profiling, ChIPseq ChIP-qPCR, gene manipulation in vitro dietary mouse models of NASH this study. Results Using unbiased label-free quantitative proteome, puromycin-labelling we observed a decrease lipotoxicity human primary hepatocytes, hepatic AML12 cells, livers from model NASH....