Abstract During the 2003–04 influenza season, 17 cases of Staphylococcus aureus community-acquired pneumonia (CAP) were reported from 9 states; 15 (88%) associated with methicillin-resistant S. (MRSA). The median age patients was 21 years; 5 (29%) had underlying diseases, and 4 (24%) risk factors for MRSA. Twelve (71%) laboratory evidence virus infection. All but 1 patient, who died on arrival, hospitalized. Death occurred in (4 MRSA). isolates available 13 (76%) (11 Toxin genes detected all...

Pertussis immunization of adults may be necessary to improve the control a rising burden disease and infection. This trial an acellular pertussis vaccine among adolescents evaluated incidence pertussis, safety, immunogenicity, protective efficacy.Bordetella infections illnesses were prospectively assessed in 2781 healthy subjects between ages 15 65 years who enrolled national multicenter, randomized, double-blind vaccine. Subjects received either dose tricomponent or hepatitis A (control)...

Journal Article Subtyping Isolates of Haemophilus influenzae Type b by Outer-Membrane Protein Profiles Get access Stephen J. Barenkamp, Barenkamp Edward Mallinckrodt Department Pediatrics, Washington University School Medicine, St. Louis, MissouriDivision Infectious Diseases, Louis Children's Hospital, Missouri Please address requests for reprints to Dr. Division 500 South Kingshighway Boulevard, 63178. Search other works this author on: Oxford Academic PubMed Google Scholar Robert S....

A collection of 242 strains Haemophilus influenzae, including 65 nontypable (unencapsulated) isolates and 177 encapsulated serotype b recovered largely from children with invasive noninvasive diseases in the United States, was characterized by electrophoretic mobilities 15 metabolic enzymes presumably encoded chromosomal genes. All were polymorphic for three to seven electromorphs, 94 distinctive multilocus genotypes (electrophoretic types [ETs]) distinguished, among which mean genetic...

A group of high-molecular-weight surface-exposed proteins nontypeable Haemophilus influenzae are major targets human serum antibody (S. J. Barenkamp and F. Bodor, Pediatr. Infect. Dis. 9:333-337, 1990). To further characterize these proteins, we cloned sequenced genes encoding two related from a prototype strain. The gene 120-kDa protein consisted 4.4-kbp open reading frame, the 125-kDa 4.6-kbp frame. first 1,259 bp were identical. Thereafter, sequences began to diverge, but overall they 80%...

Nontypable Haemophilus influenzae are Gram-negative bacilli that represent a common cause of human disease. These organisms initiate infection by colonizing the upper respiratory tract. Despite essential role colonization, bacterial determinants this process remain poorly defined. We recently identified family surface-exposed high-molecular-weight proteins nontypable H. related to filamentous hemagglutinin, critical adherence factor Bordetella pertussis. The genes encoding two such (HMW-1...

Abstract Non-typeable Haemophilus influenzae contains an N 6 -adenine DNA-methyltransferase (ModA) that is subject to phase-variable expression (random ON/OFF switching). Five modA alleles, modA2 , modA4, modA5 modA9 and modA10 account for over two-thirds of clinical otitis media isolates surveyed. Here, we use single molecule, real-time (SMRT) methylome analysis identify the DNA-recognition motifs all five these alleles. Phase variation alleles regulates multiple proteins including vaccine...

Summary Non‐typeable Haemophilus influenzae is a common respiratory pathogen and an important cause of morbidity in humans. The non‐typeable H. HMW1 HMW2 adhesins are related proteins that mediate attachment to human epithelial cells, essential step the pathogenesis disease. Secretion these requires accessory called HMW1B/HMW2B HMW1C/HMW2C. In present study, we investigated specific function HMW1C. Examination mutant constructs demonstrated HMW1C influences both size secretion HMW1....

As part of a prospective acellular pertussis (ACP) vaccine efficacy trial, 5 serum samples were obtained, over an 18-month period, from 101 ACP-vaccine recipients and 99 control subjects, to assess ACP antibody response decay. Immunoglobulin (Ig) G IgA antibodies toxin (PT), filamentous hemagglutinin (FHA), pertactin (PRN), fimbriae 2/3 (FIM) measured by enzyme-linked immunosorbant assay, titers agglutinin determined. Of the 16%–19% had preimmunization values PT that above assay's limit...

Summary We previously reported that two surface‐exposed high‐molecular‐weight proteins, HMW1 and HMW2, expressed by a prototypic strain of non‐typable Haemophilus influenzae (NTHI), mediate attachment to human epithelial cells. These proteins are members family highly immunogenic common 70–75% NTHI strains. strains lack HMW1/ HMW2‐like remain capable efficient cultured cells, suggesting the existence additional adhesion molecules. reasoned characterization from an HMW1/HMW2‐deficient might...

ABSTRACT Nontypeable Haemophilus influenzae is a common cause of human disease and initiates infection by colonizing the upper respiratory tract. In previous work we identified high-molecular-weight adhesins referred to as HMW1 HMW2, expressed nontypeable strain 12, determined that most strains H. express one or two antigenically related proteins. More recently, some lack HMW1- HMW2-like proteins instead an adhesin called Hia. present study, prevalence distribution hmw hia genes in...

The techniques of biotype determination and sodium dodecyl sulfate-polyacrylamide gel electrophoresis outer membrane protein preparations were applied to 35 epidemiologically unrelated isolates pathogenic nontypable Haemophilus influenzae. Three five obtained from the blood newborns with sepsis had concordant major older children or adults bacteremia profiles, distinct common profile neonatal strains, II. profiles remaining 5 blood, 2 isolated cerebrospinal fluid, 23 middle ear aspirates...

Acellular pertussis (aP) booster immunizations have been recommended for adolescents and older persons to enhance long-term protection possibly reduce community transmission of infections.This was a multicenter, randomized, double-blind vaccine trial in which one-half the subjects received aP hepatitis A (control subjects). All were observed almost 2 years cough illnesses, all underwent microbiologic serologic studies detection infection. Immunoglobulin G (IgG) immunoglobulin (IgA)...

Haemophilus influenzae type b isolates have been subdivided based on differences in major outer membrane protein (OMP) profiles resolved gradient and modified Laemmli sodium dodecyl sulfate-polyacrylamide gel electrophoresis systems. Although 21 subtypes observed, 86% of invasive one five common 71% three subtypes. In with two the most subtypes, OMP has an apparent molecular weight 37,000. another subtype, a cross-reactive 36,500 is observed. All proteins designated P2. Protein P2 from these...

Haemophilus influenzae is a common gram-negative pathogen that initiates infection by colonizing the upper respiratory tract epithelium. In previous work, we reported isolation of locus involved in expression short, thin surface fibrils H. type b and presented evidence promote attachment to human epithelial cells. present study, determined fibril composed one long open reading frame, designated hsf, which encodes protein (Hsf) with molecular mass approximately 240 kDa. The derived amino acid...

ABSTRACT Combinatorial cloning and expression library analysis were used to isolate human antibody Fab fragments specific for the capsular polysaccharide of Streptococcus pneumoniae serotype 23F. Thirty 23F-specific Fabs isolated from seven vaccinated donors, sequences heavy (H)- light (L)-chain variable regions determined. All individuals utilized either Vκ A23 L chain, L6 or both chains in forming combining site. paired primarily with VH3-23 H chains. more promiscuous heavy-chain usage...

The chinchilla experimental model of otitis media was used to examine the importance serum antibodies in protection against disease caused by nontypable Haemophilus influenzae. An immune pool prepared immunizing chinchillas with killed bacterial cells H. influenzae 3245. Pooled preimmune or from these immunized animals administered intravenously a group nonimmune 1 day before intrabullar challenge strain Of 5 receiving serum, developed compared 0 10 (P = 0.008). contained directed both...

Isolates of Haemophilus influenzae type b with a distinctive outer-membrane protein subtype, designated IH, were responsible for 11 13 cases invasive infection in children attending sixday-care centers secondary disease, comparison to three 14 10 single and two unrelated cases. The other caused by isolates six different subtypes. secondaryattack rate contacts youngerthan four years age exposed disease due the IH subtype was 16 per 1,000, comparedwith 1.6 1,000 after exposure subtypes than (P...

Expression of a number eukaryotic genes is regulated by long stretches tandem repeats located within the 5′ untranslated region particular gene. In this study, we describe regulatory system in Haemophilus influenzae with striking similarities to those found eukaryotes. We show that expression HMW1 and HMW2 adhesins varies based on 7-bp hmw1A hmw2A promoters. The lie between two separate transcription initiation sites exert repressive effect, such increases repeat result step-wise decreases...

We previously reported the cloning and sequencing of genes designated hmw1 hmw2 from a prototype nontypeable Haemophilus influenzae strain. The encode proteins which are related to filamentous hemagglutinin Bordetella pertussis promote attachment H. strain human epithelial cells (J. W. St. Geme III, S. Falkow, J. Barenkamp, Proc. Natl. Acad. Sci. USA 90:2875-2879, 1993). Subcloning studies suggested that correct processing these high-molecular-weight required products additional downstream...

We monitored the development of serum bactericidal antibody in eight children with acute nontypable Haemophilus influenzae otitis media and correlated its appearance against lipooligosaccharide surface-exposed outer membrane proteins infecting strains. Complement-dependent activity was absent sera but increased to titers 1:4 1:32 obtained 4 6 weeks later. Absorption anti-lipooligosaccharide antibodies from convalescent had no effect on five patients resulted small decreases titer three...

Haemophilus influenzae is a human-specific pathogen and major source of morbidity worldwide. Infection with this organism begins colonization the nasopharynx, process that probably depends on adherence to respiratory epithelium. The Hia autotransporter protein adhesin ex-pressed by subset non-typeable H. strains promotes high-level variety human epithelial cell lines. In current study, we discovered passenger domain contains two distinct binding pockets, including one at C-terminal end...

Prevention of nontypeable Haemophilus influenzae otitis media by vaccination is an important health care goal. Proteins in bacterial adherence deserve consideration as potential vaccine candidates. Two colleagues and I previously identified a family immunogenic high-molecular-weight proteins H. to human epithelial cells (J.W. St. Geme III, S. Falkow, S.J. Barenkamp, Proc. Natl. Acad. Sci. USA, 90:2875-2879, 1993). In the work described here, determined whether immunization with two such...