A5068940697- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Diagnosis and treatment of tuberculosis
- Infectious Diseases and Tuberculosis
- Public Health and Environmental Issues
- Scientific Research and Technology
- Biosimilars and Bioanalytical Methods
- HIV/AIDS Research and Interventions
- Knowledge Societies in the 21st Century
- Health and Medical Education
Universidad Católica de Santiago de Guayaquil
2020-2025
Instituto Nacional de Investigación en Salud Pública
2018-2025
Objective Tuberculosis (TB) is a major public health concern in Ecuador and Colombia, considering that both countries are high-burden TB settings. Molecular epidemiology crucial to understand the transmission dynamics of Mycobacterium tuberculosis complex (MTBC) identify active clusters regional importance. Methods We studied potential between Colombia through analysis population structure MTBC lineages circulating Ecuadorian province Esmeraldas at border with Colombia. A total 105 strains...
The Mycobacterium tuberculosis complex (MTBC), the causative agent of (TB), remains a significant global health challenge, with an estimated 10.8 million cases diagnosed and 1.25 deaths in 2023, according to WHO. In this context, enhancing TB case detection using more sensitive diagnostic methods is essential. Here, we compared performance two commercial real-time polymerase chain reaction (PCR) kits reference standards smear microscopy, culture, Gene Xpert. By analyzing 402 clinical...
Abstract Tuberculosis (TB) is a significant public health problem in Ecuador with an incidence of 43 per 100,000 inhabitants and estimated multidrug-resistant-TB prevalence all TB cases 9%. Genotyping Mycobacterium tuberculosis (MTBC) important to understand regional transmission dynamics. This study aims describe the main MTBC lineages sublineages circulating country. A representative sample 373 strains from 22 provinces Ecuador, data comprising geographic origin drug susceptibility, were...
Tuberculosis (TB) is a major public health concern in Ecuador and Peru, both settings of high burden drug resistance TB. Molecular epidemiology tools are important to understand the transmission dynamics Mycobacterium tuberculosis Complex (MTBC) track active clusters regional importance. This study first address TB between Peru through population structure MTBC lineages circulating Ecuadorian border province "El Oro". A total number 56 strains from this for years 2012-2015 were included...
Infections caused by mycobacteria, including Mycobacterium tuberculosis complex (MTBC) and non-tuberculous mycobacteria (NTM), are a major public health issue worldwide. An accurate diagnosis of mycobacterial species is challenge for surveillance treatment, particularly in high-burden settings usually associated with low- middle-income countries. In this study, we analyzed the clinical performance two commercial PCR kits designed identification differentiation MTBC NTM, available setting...
The objective of this study was to compare the diagnostic yield Kudoh-Ogawa (K-O) swab method for culturing Mycobacterium tuberculosis from clinical samples with standard Petroff–Lowenstein-Jensen (P-LJ) procedure. A total 2,287 sputum and 685 extrapulmonary specimens were processed both decontamination methods compared M. detection rate, recovery colonies, culture contamination.
The Beijing family, the most successful Mycobacterium tuberculosis lineage, is considered hypervirulent, associated with clustering and has a strong association multidrug-resistant tuberculosis. strains have spread worldwide also to Latin America. Genotyping of countrywide collection 380 M. from Ecuador, 24-loci mycobacterial interspersed repetitive units-variable number tandem repeats (MIRU-VNTR), revealed only six strains, but four these were MDR-TB. There was no as all had very distinct...
The aim of this study was to characterize the most frequent mutations associated with rifampicin (RIF) and isoniazid (INH) resistance Mycobacterium tuberculosis isolates from Ecuador. Sequence analysis 40 strains, resistant for drugs INH, RIF, or both showed that 31 strains 20 (64.5%) carried a mutation in katG gene (codon 315). Eight INH-resistant at codon 463. This katG463 mutation, considered phylogenetic marker, exclusively found not 121 INH-susceptible strains. Of 35 33 (93.9%) had hot...
A major sublineage within the
Background: Strains of the Beijing sublineage Mycobacterium tuberculosis have caused large outbreaks tuberculosis, often involving multidrug resistance strains and this genetically highly conserved family predominates in some geographic areas. For most countries Latin America, no country-wide studies about prevalence lineage are available. Methods: In study, we determine Ecuador, using a nation-wide sample 991 isolates from years 2014–2016 with strains, case-related-proportional...
Background: The incidence of multidrug resistance (MDR) acquired to antituberculous Mycobacterium tuberculosis drugs in Ecuador is approximately 27.83%. Objective: To detect mutations associated with drug drug-resistant strains M. using PCR-RFLP and real-time PCR.Materials methods: In (DR) isolated from samples 18 Ecuadorian provinces (2006-2012), the rpoB KatG genes that lead rifampicin isoniazid, respectively, were analyzed. DNA wasamplified by Polymerase Chain Reaction (PCR), being then...