Phase imaging with a high-resolution wavefront sensor is considered. This based on quadriwave lateral shearing interferometer mounted non-modified transmission white-light microscope. The measurement technology explained both in the scope of wave optics and geometrical order to discuss its implementation conventional In particular we consider effect non spatially coherent source phase-image signal-to-noise ratio. Precise measurements phase-shift introduced by microscopic beads or giant...

The temperature distribution throughout arrays of illuminated metal nanoparticles is investigated numerically and experimentally. two cases continuous femtosecond-pulsed illumination are addressed. In the case illumination, distinct regimes evidenced: a confinement regime, where increase remains confined at vicinity each nanosource heat, delocalization uniform whole nanoparticle assembly despite heat sources' nanometric size. We show that occurrence one regime or another simply depends on...

Under illumination, metal nanoparticles can turn into ideal nanosources of heat due to enhanced light absorption at the plasmonic resonance wavelength. In this article, we aim providing a comprehensive description generation microbubbles in liquid occurring around under continuous illumination. We focus on common situation where are located solid substrate and immersed water. Experimentally, evidenced series singular phenomena: (i) bubble lifetime after heating reach several minutes, (ii)...

We introduce an optical microscopy technique aimed at characterizing the heat generation arising from nanostructures, in a comprehensive and quantitative manner. Namely, permits (i) mapping temperature distribution around source of heat, (ii) power density delivered by source, (iii) retrieving absolute absorption cross section light-absorbing structures. The is based on measure thermal-induced refractive index variation medium surrounding heat. measurement achieved using association regular...

Significance Central biological processes in cells and tissues are intrinsically governed by the structural order of biomolecular assemblies. It is thus a key factor to decipher how these assemblies organize complex molecular organizations, from nanometric macroscopic scale. Polarized microscopy can access such information; however, signals spatially averaged over optical diffraction limit contaminated fluorophores’ orientational flexibility their linker biomolecules. By bringing polarized...

Single-cell dry mass measurement is used in biology to follow cell cycle, address effects of drugs, or investigate metabolism. Quantitative phase imaging technique with quadriwave lateral shearing interferometry (QWLSI) allows measuring mass. The very simple set up, as it integrated a camera-like instrument. It simply plugs onto standard microscope and uses white light illumination source. Its working principle first explained, from image acquisition automated segmentation algorithm...

We report a step toward scanning endomicroscopy without distal optics. The focusing of the beam at end fiber bundle is achieved by imposing parabolic phase profile across exit face with aid spatial light modulator. achieve video-rate images galvanometric tilt proximal end. approach made possible bundle, designed to have very low coupling between cores.

Abstract Meeting the nanometre resolution promised by super-resolution microscopy techniques (pointillist: PALM, STORM, scanning: STED) requires stabilizing sample drifts in real time during whole acquisition process. Metal nanoparticles are excellent probes to track lateral as they provide crisp and photostable information. However, achieving axial super-localization is still a major challenge, diffraction imposes large depths-of-fields. Here we demonstrate fast full three-dimensional of...

We report a first demonstration of two-photon endoscopic imaging with lensless endoscope. The endoscope probe is double-clad bundle single-mode fibers; point excitation and scanning achieved by coherent combining femtosecond light pulses propagating in the back-scattered signal collected through multi-mode inner cladding. demonstrate on test sample rhodamine 6G crystals.

Wavefront sensors are usually based on measuring the wavefront derivatives. The most commonly used approach to quantitatively reconstruct uses discrete Fourier transform, which leads artifacts when phase objects located at image borders. We propose here a simple avoid these duplication and antisymmetrization of derivatives data, in derivative direction, before integration. This completely erases border effects by creating continuity differentiability edge image. finally compare this...

We describe a new technique based on the use of high-resolution quadri-wave lateral shearing interferometer to perform quantitative linear retardance and birefringence measurements biological samples. The system combines phase images with varying polarization excitation create images. This is compatible living samples gives information about local structure their anisotropic components. applied our approach collagen fibers leading value (3.4 ± 0.3) · 10(-3) cells, showing that cytoskeleton...

Solvothermal synthesis, denoting chemical reactions occurring in metastable liquids above their boiling point, normally requires the use of a sealed autoclave under pressure to prevent solvent from boiling. This work introduces an experimental approach that enables solvothermal synthesis at ambient open reaction medium. The is based on gold nanoparticles deposited glass substrate and acting as photothermal sources. To illustrate approach, selected hydrothermal involves formation indium...

This article introduces an optical microscopy technique for the characterization of two-dimensional (2D) materials. The is based on use quadriwave lateral shearing interferometry (QLSI), a quantitative phase imaging that allows both intensity and incoming light beam. shows how QLSI can be used to (i) image 2D materials with high contrast transparent substrates, (ii) detect presence residues coming from fabrication process, (iii) map complex conductivity refractive index by processing images...

This paper introduces a procedure aimed to quantitatively measure the optical properties of nanoparticles, namely complex polarizability and extinction, scattering, absorption cross sections, simultaneously. The method is based on processing intensity wavefront images light beam illuminating nanoparticle interest. Intensity measurements are carried out using quadriwave lateral shearing interferometry, quantitative phase imaging technique with high spatial resolution sensitivity. does not...

An optical metasurface consists of a dense and usually nonuniform layer scattering nanostructures behaving as continuous extremely thin component with predefined phase, transmission reflection profiles. To date, various sorts metasurfaces (metallic, dielectric, Huygens-like, Pancharatman-Berry, etc.) have been introduced to design ultrathin lenses, beam deflectors, holograms, or polarizing interfaces. Their actual efficiencies depend on the ability predict their properties fabricate...

A complete set of cavity modes in planar dielectric microcavities is presented which naturally includes guided modes. We show that most these orthonormal fields can be derived from a coherent superposition plane waves incoming on the stack air and substrate. Spontaneous emission dipole located inside microcavity analyzed, terms Derivation radiation pattern substrate presented. The power emitted into also determined. Finally, numerical analysis radiative properties an erbium atom...

We present a quadriwave lateral shearing interferometer used as wavefront sensor and mounted on commercial non-modified transmission white-light microscope quantitative phase imaging technique. The setup is designed to simultaneously make measurements with both fluorescence modes: enables enhanced contrasted visualization of the cell structure including intracellular organelles, while allows complete precise identification each component. After characterization measurement reliability...

We developed a technique based on the use of smart patterns gold nanoparticles under illumination to generate any temperature field microscale.

Abstract Laser heating of individual cells in culture recently led to seminal studies cell poration, fusion, migration, or nanosurgery, although measuring the local temperature increase such experiments remains a challenge. Here, laser‐induced dynamical control heat‐shock response is demonstrated at single level, enabled by use light‐absorbing gold nanoparticles as nanosources heat and mapping technique based on quadriwave lateral shearing interferometry (QLSI) measurements. As it...

We report on the development of a mitigation process to prevent growth UV nanosecond laser-initiated damage sites under successive irradiations fused silica components. The developed is based fast microablation as it has been proposed by Bass et al. [Bass al., Proc. SPIE 7842, 784220 (2010)]. This accomplished displacement CO2 laser spot with galvanometer beam scanner form crater typical conical shape mitigate large (millimetric) and deep (few hundred microns) sites. present experimental...

Laser stereolithography deals with the manufacture of 3D objects that are made by space-resolved laser-induced polymerization. In order to obtain micro-objects, we developed a new microstereolithography apparatus based on use dynamic mask generator which allows complete layer only one irradiation, part being manufactured layer. This process is compared broad-band visible light source, leads elimination speckle effects resulting from conventional laser beam, and liquid crystal display as...

The combination of a microstereolithography process and high‐load ceramic suspension is used to produce complex three‐dimensional alumina microparts. object (see Figure) produced designed for microfluidic applications, but the versatility technique generally applicable preparation microcomponents numerous applications.