A5023157165- Advanced Fluorescence Microscopy Techniques
- Near-Field Optical Microscopy
- Advanced Electron Microscopy Techniques and Applications
- Cell Image Analysis Techniques
- Force Microscopy Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Digital Holography and Microscopy
- Nanoplatforms for cancer theranostics
- Photoacoustic and Ultrasonic Imaging
- Bacterial biofilms and quorum sensing
- Supramolecular Self-Assembly in Materials
- Antimicrobial Resistance in Staphylococcus
- Cellular Mechanics and Interactions
- Functional Brain Connectivity Studies
- Endoplasmic Reticulum Stress and Disease
- Optical Coherence Tomography Applications
- Single-cell and spatial transcriptomics
- Cellular transport and secretion
- Alzheimer's disease research and treatments
- Microfluidic and Bio-sensing Technologies
- Scientific Computing and Data Management
- Research Data Management Practices
- Genetic Neurodegenerative Diseases
- Advanced biosensing and bioanalysis techniques
- Electron and X-Ray Spectroscopy Techniques
HealthInsight
2020-2023
École Normale Supérieure Paris-Saclay
2023
Institut des Sciences Moléculaires d'Orsay
2015-2021
Université Paris-Saclay
2018-2021
Centre National de la Recherche Scientifique
2015-2021
Université Paris-Sud
2015-2019
Abstract Non-uniform illumination limits quantitative analyses of fluorescence imaging techniques. In particular, single molecule localization microscopy (SMLM) relies on high irradiances, but conventional Gaussian-shaped laser restricts the usable field view to around 40 µm × µm. We present Adaptable Scanning for Tunable Excitation Regions (ASTER), a versatile technique that generates uniform and adaptable illumination. ASTER is also highly compatible with optical sectioning techniques such...
Abstract Meeting the nanometre resolution promised by super-resolution microscopy techniques (pointillist: PALM, STORM, scanning: STED) requires stabilizing sample drifts in real time during whole acquisition process. Metal nanoparticles are excellent probes to track lateral as they provide crisp and photostable information. However, achieving axial super-localization is still a major challenge, diffraction imposes large depths-of-fields. Here we demonstrate fast full three-dimensional of...
Determining how cells generate and transduce mechanical forces at the nanoscale is a major technical challenge for understanding of numerous physiological pathological processes. Podosomes are submicrometer cell structures with columnar F-actin core surrounded by ring adhesion proteins, which possess singular ability to protrude into probe extracellular matrix. Using protrusion force microscopy, we have previously shown that single podosomes produce local protrusions on environment. However,...
Daptomycin is a last-resort membrane-targeting lipopeptide approved for the treatment of drug-resistant staphylococcal infections, such as bacteremia and implant-related infections. Although cases resistance to this antibiotic are rare, increasing numbers clinical, in vitro, animal studies report failure, notably against Staphylococcus aureus The aim study was identify features daptomycin its target bacteria that lead failure. We show bactericidal activity S. varies significantly with growth...
Abstract Here, we present a 3D localization-based super-resolution technique providing slowly varying localization precision over 1 μm range with precisions down to 15 nm. The axial is performed through combination of point spread function (PSF) shaping and supercritical angle fluorescence (SAF), which yields absolute information. Using dual-view scheme, the detection decoupled from lateral optimized independently provide weakly anisotropic resolution imaging range. This method can be...
We propose a straightforward sample-based technique to calibrate the axial detection in 3D single-molecule localization microscopy. Using microspheres coated with fluorescent molecules, calibration curves of point spread function-shaping or intensity-based measurements can be obtained over imaging depth range. This experimental method takes into account effect spherical aberration without requiring computational correction. demonstrate its efficiency for astigmatic 1.2 μm range above coverslip.
Single-molecule localization microscopy (SMLM) can reach sub-50 nm resolution using techniques such as stochastic optical reconstruction (STORM) or DNA-point accumulation for imaging in nanoscale topography (PAINT). Here we implement two approaches faster multicolor SMLM by splitting the emitted fluorescence toward cameras: simultaneous two-color DNA-PAINT (S2C-DNA-PAINT) that images spectrally separated red and far-red imager strands on each camera, spectral demixing dSTORM (SD-dSTORM)...
Abstract Single Molecule Localization Microscopy (SMLM) is a straightforward approach to reach sub-50 nm resolution using techniques such as Stochastic Optical Reconstruction (STORM) or DNA-Point Accumulation for Imaging in Nanoscale Topography (PAINT), and resolve the arrangement of cellular components their native environment. However, SMLM acquisitions are slow, particularly multicolor experiments where channels usually acquired sequence. In this work, we evaluate two approaches speed-up...
Abstract Strategies have been developed in LIDAR to perform distance measurements for non-coherent emission sparse samples based on excitation modulation. Super-resolution fluorescence microscopy is also striving axial localization but through entirely different approaches. Here we revisit the amplitude modulated approach reach nanometric precision and successfully adapt it bring distinct advantages super-resolution microscopy. The pattern performed by interference enabling decoupling...
Abstract Quantitative analyses in classical fluorescence microscopy and Single Molecule Localization Microscopy (SMLM) require uniform illumination over the field of view; ideally coupled with optical sectioning techniques such as Total Internal Reflection Fluorescence (TIRF) to remove out focus background. In SMLM, high irradiances (several kW/cm ² ) are crucial drive densely labeled sample into single molecule regime, conventional gaussian-shaped lasers will typically restrain usable view...
We developed a 3D localization-based super-resolution technique providing slowly varying localization precision over 1 μm range with precisions down to 15 nm. The axial is performed through combination of point spread function (PSF) shaping and supercritical angle fluorescence (SAF), which yields absolute information. Using dual-view scheme, the detection decoupled from lateral optimized independently provide weakly anisotropic resolution imaging range. This method can be readily implemented...
Genetic factors are known to contribute Late Onset Alzheimer’s disease (LOAD) but their contribution pathophysiology, specially prodomic phases accessible therapeutic approaches far be understood. To translate genetic risk of Alzheimer's (AD) into mechanistic insight, we generated transgenic mouse lines that express a ~195 kbp human BAC includes only BIN1, gene associated LOAD. This model gives modest BIN1 overexpression, dependent the number copies. At 6 months age, detected impaired...
Novel insights and more powerful analytical tools can emerge from the reanalysis of existing data sets, especially via machine learning methods. Despite widespread use single molecule localization microscopy (SMLM) for super-resolution bioimaging, underlying are often not publicly accessible. We developed ShareLoc ( https://shareloc.xyz ), an open platform designed to enable sharing, easy visualization SMLM data. discuss its features show how sharing improve performance robustness image...