A5034748646- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Neuropeptides and Animal Physiology
- Protein Hydrolysis and Bioactive Peptides
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cell Adhesion Molecules Research
- Calpain Protease Function and Regulation
- Enzyme Production and Characterization
- Iron Metabolism and Disorders
- Protein Interaction Studies and Fluorescence Analysis
- Trypanosoma species research and implications
- Blood Coagulation and Thrombosis Mechanisms
- Metal complexes synthesis and properties
- Renin-Angiotensin System Studies
- Academic Research in Diverse Fields
- Cellular transport and secretion
- Selenium in Biological Systems
- Adenosine and Purinergic Signaling
- Molecular Junctions and Nanostructures
- Organometallic Compounds Synthesis and Characterization
- Tailings Management and Properties
- Phytase and its Applications
- Pancreatic function and diabetes
- Fire effects on ecosystems
- Enzyme function and inhibition
University of Aveiro
2024
Weatherford College
2018
University of Regina
2018
Kimberly-Clark (Canada)
2018
Universidade Federal de São Paulo
2011-2013
Universidade de Mogi das Cruzes
1999-2011
Universidade Federal do Paraná
2010
Eletrobras (Brazil)
2002
Ann Arbor Center for Independent Living
2002
University of Michigan
2002
It has been shown that lysosomal cysteine proteinases, specially cathepsin B, implicated in a variety of diseases involving tissue remodeling states, such as inflammation, parasite infection, and tumor metastasis, by degradation extracellular matrix components. Recently, we have heparin heparan sulfate bind to papain specifically; this interaction induces an increase its alpha-helix content stabilizes the enzyme structure even at alkaline pH (Almeida, P. C., Nantes, I. L., Rizzi, C. A.,...
Endopeptidase 24.15 (EC; ep24.15), neurolysin ep24.16), and angiotensin-converting enzyme ACE) are metallopeptidases involved in neuropeptide metabolism vertebrates. Using catalytically inactive forms of ep24.15 ep24.16, we have identified new peptide substrates for these enzymes. The enzymatic activity ep24.16 was inactivated by site-directed mutagenesis amino acid residues within their conserved HEXXH motifs, without disturbing secondary structure or binding ability, as shown circular...
<i>Trypanosoma cruzi</i> activates the kinin pathway through activity of its major cysteine proteinase, cruzipain. Because kininogen molecules may be displayed on cell surfaces by binding to glycosaminoglycans, we examined whether ability cruzipain release kinins from high molecular weight (HK) is modulated heparan sulfate (HS). Kinetic assays show that HS reduces proteinase inhibitory (<i>K</i> <sub>i</sub> <sub>app</sub>) HK about 10-fold. Conversely, catalytic efficiency kinin-related...
Kinetic data for the hydrolysis by human tissue kallikrein of fluorogenic peptides with o-aminobenzoyl-Phe-Arg (Abz-FR) as acyl group and different leaving groups demonstrate that interactions S'1, S'2 S'3 subsites are important cleavage efficiency. In addition, studies on kininogen sequence spanning scissile Met-Lys bond [Abz-M-I-S-L-M-K-R-P-N-(2,4-dinitrophenyl)ethylenediamine] analogues residues at positions P'1, P'2 P'3 showed (a) presence a proline residue kallikrein-binding sites S2 to...
Papain is considered to be the archetype of cysteine proteinases. The interaction heparin and other glycosaminoglycans with papain may representative many mammalian proteinase-glycosaminoglycan interactions that can regulate function this class proteinases in vivo. conformational changes structure due glycosaminoglycan were studied by circular dichroism spectroscopy, enzyme behavior kinetic analysis, monitored fluorogenic substrate. presence significantly increases α-helix content papain....
Background Cysteine protease B is considered crucial for the survival and infectivity of Leishmania in its human host. Several microorganism pathogens bind to heparin-like glycosaminoglycans chains proteoglycans at host-cell surface promote their attachment internalization. Here, we have investigated influence heparin upon mexicana cysteine rCPB2.8 activity. Methodology/Principal Findings The data analysis revealed that presence affects all steps enzyme reaction: (i) it decreases 3.5-fold k1...
Heparin has been shown to regulate human neutrophil elastase (HNE) activity. We have assessed the regulatory effect of heparin on Tissue Inhibitor Metalloproteases-1 [TIMP-1] hydrolysis by HNE employing recombinant form TIMP-1 and correlated FRET-peptides comprising cleavage site. accelerates 2.5-fold HNE. The kinetic parameters this reaction were monitored with aid a FRET-peptide substrate that mimics site in pre-steady-state conditionsby using stopped-flow fluorescence system. exhibits...
Wildfire ignitions are often linked to environmental and climatic factors, but human behavior plays a critical role, particularly in rural southern Europe. However, tools quantify the probability of human-caused lacking. This study addresses this by developing wildfire ignition index focused on mainland Portugal, region historically vulnerable wildfires. Statistical analyses, including multicollinearity checks Generalized Linear Model, were used analyze data, while geospatial analyses...
The metalloendopeptidase 24.15 (EP24.15) is ubiquitously present in the extracellular environment as a secreted protein. Outside cell, this enzyme degrades several neuropeptides containing from 5 to 17 amino acids (e.g. gonadotropin releasing hormone, bradykinin, opioids and neurotensin). constitutive secretion of EP24.15 glioma C6 cells was demonstrated be stimulated linearly by reduced concentrations calcium. In report we demonstrate that calcium concentration has no effect on total amount...
Cathepsin B is a lysosomal thiolprotease that, because of its colocalization with renin and ability to activate prorenin, has been proposed as prorenin processing enzyme. To characterize the biochemical aspect this potential cathepsin activity in more detail, we synthesized assayed human internally quenched fluorescent peptide Abz-FSQPMKRLTLGNTTQ-EDDnp (Abz, ortho-aminobenzoic acid group EDDnp, N-¿2, 4-dinitrophenyl-ethylenediamine quencher group) that contains 7 amino acids for each side...
We combined fluorogenic substrates or internally quenched fluorescent peptides with specific inhibitors in the pH profile of proteolytic activity experiments order to detect activities lysates MDCK cells. Hydrolytic related cathepsin B, L, and D were observed. Serine-proteinase was not detected; however, we clearly demonstrated presence a thiol-metallo-endo-oligopeptidase, also called thimet-oligopeptidase (TOP). This peptidase from cells has substrate inhibitor specificities as well an...
We combined fluorogenic substrates or internally quenched fluorescent peptides with specific inhibitors in the pH profile of proteolytic activity experiments order to detect activities lysates MDCK cells. Hydrolytic related cathepsin B, L, and D were observed. Serine-proteinase was not detected; however, we clearly demonstrated presence a thiol-metallo-endo-oligopeptidase, also called thimet-oligopeptidase (TOP). This peptidase from cells has substrate inhibitor specificities as well an...
This paper reports on the development of 440 kV Bauru substation security control scheme, Brazil, as well all changes in physical layout and protection systems that are to be made before installation this scheme.