A5007921756- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- Pancreatic function and diabetes
- Glycosylation and Glycoproteins Research
- Cellular Mechanics and Interactions
- Photoreceptor and optogenetics research
- Advanced Memory and Neural Computing
- Parkinson's Disease Mechanisms and Treatments
- Vector-borne infectious diseases
- Mitochondrial Function and Pathology
- Cellular transport and secretion
- Peptidase Inhibition and Analysis
- Neuroscience and Neuropharmacology Research
- Endoplasmic Reticulum Stress and Disease
- Diabetes and associated disorders
- Viral Infections and Vectors
- 3D Printing in Biomedical Research
- Bioinformatics and Genomic Networks
- Galectins and Cancer Biology
- Advanced Glycation End Products research
- Genetic Neurodegenerative Diseases
- Machine Learning in Bioinformatics
- Nerve injury and regeneration
- Extracellular vesicles in disease
- Advanced Biosensing Techniques and Applications
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2017-2025
Instituto Nacional de Engenharia Biomédica
2024
National Institute of Engineering, Technology and Innovation
2024
Universidade do Porto
2017-2022
University of Aveiro
2010-2016
Celulose Beira Industrial (Portugal)
2014
Deleted Institution
2014
Selangor Business School
2014
Biopeptide (United States)
2008-2011
Philipps University of Marburg
2010
It has long been accepted that mitochondrial function and morphology is affected in Parkinson's disease, can be directly related to its morphology. So far, morphological alterations studies, the context of this neurodegenerative have performed through microscopic methodologies. The goal present work address if modifications mitochondrial-shaping proteins occurring disorder implications other cellular pathways, which might constitute important pathways for disease progression. To do so, we...
Abstract The characterization of industrial yeast strains by examining their metabolic footprints (exometabolomes) was investigated and compared to genome‐based discriminatory methods. A group nine brewing yeasts studied comparing footprints, genetic fingerprints comparative genomic hybridization profiles. Metabolic footprinting carried out both direct injection mass spectrometry (DIMS) gas chromatography time‐of‐flight (GC–TOF–MS), with data analysed principal components analysis (PCA)...
ABSTRACT Mucosal immunization is advantageous over other routes of antigen delivery because it can induce both mucosal and systemic immune responses. Our goal was to develop a vehicle based on bacteria generally regarded as safe, such Lactobacillus spp. In this study, we used the Lyme disease mouse model proof concept. We demonstrate that an oral vaccine live recombinant plantarum protects mice from tick-transmitted Borrelia burgdorferi infection. method expressing antigens in L. induces...
Lyme disease is caused by the spirochete Borrelia burgdorferi. The enzootic cycle of this pathogen requires that Ixodes spp. acquire B. burgdorferi from infected wildlife reservoirs and transmit it to other uninfected wildlife. At present, there are no effective measures control burgdorferi; human vaccine available, existing vector generally not acceptable public. However, if could be eliminated its reservoir hosts or ticks feed on them, would broken, incidence decrease. We developed...
Abstract Objective . Recent technological advances are revealing the complex physiology of axon and challenging long-standing assumptions. Namely, while most action potential (AP) initiation occurs at initial segment in central nervous system neurons, distal parts has been reported to occur both physiological pathological conditions. The functional role these ectopic APs, if exists, is still not clear, nor its impact on network activity dynamics. Approach Using an electrophysiology platform...
Neurons are specialized cells for information transmission and processing. In fact, many neurologic disorders directly linked not to cellular viability/homeostasis issues but rather specific anomalies in electrical activity dynamics. Consequently, therapeutic strategies based on the direct modulation of neuronal have been producing remarkable results, with successful examples ranging from cochlear implants deep brain stimulation. Developments these implantable devices hindered, however, by...
Abstract Understanding the intricate structure-function relationships of neuronal circuits is crucial for unraveling how brain achieves efficient information transfer. In specific regions, like hippocampus, neurons are organized in layers and form unidirectional connectivity, which thought to help ensure controlled signal flow processing. recent years, researchers have tried emulating these structural principles by providing cultured with asymmetric environmental cues, namely microfluidics’...
Helicobacter pylori, a stomach-colonizing Gram-negative bacterium, is the main etiological factor of various gastroduodenal diseases, including gastric adenocarcinoma. By establishing life-long infection mucosa, H. pylori continuously activates host-signaling pathways, in particular those associated with receptor tyrosine kinases. Using two different epithelial cell lines, we show that targets kinase EPHA2. For long periods time post-infection, induces EPHA2 protein downregulation without...
Pex11 proteins are involved in membrane elongation and division processes associated with the multiplication of peroxisomes. Human Pex11pβ has recently been linked to a new disorder affecting peroxisome morphology dynamics. Here, we have analyzed exact topology Pex11pβ. Studies an epitope-specific antibody protease protection assays show that is integral protein two transmembrane domains flanking internal region exposed peroxisomal matrix N- C-termini facing cytosol. A glycine-rich within...
Abstract Background Different pathologies, affecting the skeletal system, were reported to display altered bone and/or cartilage innervation profiles leading deregulation of tissue homeostasis. The patterning peripheral is achieved through tissue-specific expression attractive or repulsive axonal guidance cues in specific space and time frames. During last decade, emerging findings attributed extracellular vesicles (EV) trading a central role innervation. However, date, contribution EV...
Zymogen granules (ZG) are specialized storage organelles in the exocrine pancreas that allow sorting, packaging, and regulated apical secretion of digestive enzymes. As there is a critical need for further understanding key processes to develop new therapeutic options medicine, we applied suborganellar proteomics approach identify peripheral membrane-associated ZG proteins. We focused on analysis "basic" group (pH range 6.2−11) with about 46 spots among which 44 were identified by tandem...
Closed-loop neuronal stimulation has a strong therapeutic potential for neurological disorders such as Parkinson’s disease. However, at the moment, standard protocols rely on continuous open-loop and design of adaptive controllers is an active field research. Delayed feedback control (DFC), popular method used to chaotic systems, been proposed closed-loop technique desynchronisation populations but, so far, was only tested in computational studies. We implement DFC first time access its...
Cardiac dysfunction secondary to cancer may exert a negative impact in patients’ tolerance therapeutics, quality of life, and survival. The aim this study was evaluate the potential therapeutic effect exercise training on heart setting cancer, after diagnosis. Thus, molecular pathways harbored mitochondria from murine model chemically-induced urothelial carcinoma submitted 8-weeks high intensity treadmill were characterized using mass spectrometry-based proteomics. Data highlight protective...
Parkinson’s disease’s etiology is unknown, although evidence suggests the involvement of oxidative modifications intracellular components in disease pathobiology. Despite known extracellular matrix physiology and disease, influence stress on has been neglected. The chemical that might accumulate due to their long half-live low amount antioxidants could also contribute explain ineffective cellular therapies. enriched striatal from a mouse model was characterized by Raman spectroscopy. We...
Abstract Astrocytes, primarily viewed only as supportive units, are now emerging active players in the information processing of brain. Accumulated evidence supports that bidirectional communication between astrocytes and neurons maintains complex animal behaviours such memory formation decision-making. The lack characterisation astrocytic electrophysiology is, our opinion, associated with early idea a passive electrical nature astrocytes, opposition to electrically neurons. A better...