A5062897620- Cancer Genomics and Diagnostics
- Genomic variations and chromosomal abnormalities
- Breast Cancer Treatment Studies
- Carcinogens and Genotoxicity Assessment
- Microtubule and mitosis dynamics
- Estrogen and related hormone effects
- Occupational and environmental lung diseases
- Animal Genetics and Reproduction
- HER2/EGFR in Cancer Research
- DNA Repair Mechanisms
- Gene expression and cancer classification
- Genetically Modified Organisms Research
- Pesticide Exposure and Toxicity
- Genomics and Chromatin Dynamics
- Cancer-related Molecular Pathways
- Advanced Breast Cancer Therapies
- Molecular Biology Techniques and Applications
- Air Quality and Health Impacts
- Lymphoma Diagnosis and Treatment
- Schizophrenia research and treatment
- 3D Printing in Biomedical Research
- Sarcoma Diagnosis and Treatment
- Hypothalamic control of reproductive hormones
- Chronic Lymphocytic Leukemia Research
- Telomeres, Telomerase, and Senescence
Pedagogical and Technological University of Colombia
2017-2025
University of Turin
2013-2016
Universidad del Rosario
2010-2016
Pathogenic germline variants in the BAP1 tumor suppressor gene can cause a cancer syndrome called predisposition (BAP1-TPDS), which is characterized by to mesothelioma, melanoma, renal cell carcinoma, basal and other tumors. Other genes that may predispose mesothelioma are CDKN2A DNA repair genes. Asbestos exposure has often been reported patients with malignant pleural (MPM) BAP1, but this never quantified. We aimed search for among 25 new Italian probands suspected BAP1-TPDS, summarize...
The significance of androgen receptor (AR) in breast cancer (BC) management is not fully defined, and it still ambiguous how the level AR expression influences oestrogen receptor-positive (ER+) tumours. aim present study was to analyse prognostic impact AR/ER ratio, evaluated by immunohistochemistry (IHC), correlating this value with clinical, pathological molecular characteristics. We retrospectively selected a cohort 402 ER+BC patients. On each tumour, IHC analyses for AR, ER, PgR, HER2...
The MCF7 (ER+/HER2-), T47D BT474 (ER+/HER2+) and SKBR3 (ER-/HER2+) breast cancer cell lines are widely used in research as paradigms of the luminal HER2 phenotypes. Although they have been subjected to cytogenetic analysis, their chromosomal abnormalities not carefully characterized, differential profiles yet established. In addition, techniques such comparative genomic hybridization (CGH), microarray-based CGH multiplex ligation-dependent probe amplification (MLPA) described specific...
MDM2 amplification represents the leading oncogenic pathway and diagnostic hallmark of liposarcoma, whose assessment is based on Fluorescence In Situ Hybridization (FISH) analysis. Despite its relevance, no univocal interpretation criteria regarding FISH assessments have been established so far, to several different approaches potential misinterpretations. This study aims address most common issues proposes troubleshooting guidelines for by FISH. We retrospectively retrieved 51 liposarcomas,...
Triple negative breast cancer (TNBC) is a heterogeneous disease considered the most aggressive among all diagnosed cancers. The Luminal Androgen Receptor (LAR) category has been recognized as distinct entity (subtype), observed in around 15% of TNBCs but currently there no complete overlap to clearly distinguish them from other TNBC counterparts. With aim better establish morphological, immunohistochemical and molecular features LAR tumors, gene signatures expression was evaluated 42 well...
Chromosomal instability (CIN), defined by variations in the number or structure of chromosomes from cell to cell, is recognized as a distinctive characteristic cancer associated with ability tumors adapt challenging environments. CIN has been source genetic variation that leads clonal heterogeneity (CH). Recent findings suggest potential association between and CH prognosis BC patients, particularly expressing epidermal growth factor receptor 2 (HER2+). In fact, information on role other...
Primary cultures represent an invaluable tool to set up functional experimental conditions; however, creation of tissue from solid tumors is troublesome and often unproductive. Several features can affect the success rate primary cultures, including technical issues pre-analytical procedures employed in surgical theaters pathology laboratories. We have recently introduced a new method collection, transfer, preservation specimens that requires immediate vacuum sealing excised at theaters,...
The co-expression of androgen (AR) and estrogen (ER) receptors, in terms higher AR/ER ratio, has been recently associated with poor outcome ER-positive (ER+) breast cancer (BC) patients. aim this study was to analyze if the biological aggressiveness, underlined ER+ BC tumors could be due expression genes related cell proliferation. On a cohort 47 patients, ratio assessed by immunohistochemistry mRNA analysis. level five gene proliferation markers defined through TaqMan®-qPCR assays. Results...
In diagnostic pathology, HER2 status is determined in interphase nuclei by fluorescence situ hybridization (FISH) with probes for the gene and chromosome 17 centromere (CEP17). The latter probe used as a surrogate copies, however (Chr17) frequently rearranged. frequency type of specific structural Chr17 alterations breast cancer have been studied using comparative genomic spectral karyotyping, but not fully detailed. Actually, balanced rearrangements (e.g. translocations or inversions) low...
The selective estrogen receptor (ER) modulator tamoxifen (TAM) has become the standard therapy for treatment of ER+ breast cancer patients. Despite obvious benefits TAM, a proportion patients acquire resistance to treatment, and this is significant clinical problem. Consequently, identification possible mechanisms involved in TAM-resistance should help development new therapeutic targets. In study, we present vitro data using panel different cell lines demonstrate modulatory effect TAM on...
Painters are exposed to an extensive variety of harmful substances like aromatic hydrocarbons used as solvents and paint removers, some which have shown clastogenic activity. These constitute a complex mixture chemicals contain well-known genotoxicants, such Benzene, Toluene Xylene. Thus, chronic occupational exposure may be considered possess genotoxic risk. In Colombia the information available around damage (Chromosomal DNA damage) in car shop workers is limited knowledge this could...
Chromosomal instability (CIN), defined by variations in the number or structure of chromosomes from cell to cell, is recognized as a distinctive characteristic cancer, associated with ability tumors adapt challenging environments. CIN has been source genetic variation that leads clonal heterogeneity (CH). Recent findings suggest potential association between and CH prognosis BC patients, particularly expressing epidermal growth factor receptor 2 (HER2+). In fact, information on role other...
For several years, cell-free DNA has been emerging as an important biomarker for non-invasive diagnostic in a wide range of clinical conditions and diseases. The limited information available on the genotoxic effects associated with occupational exposure to car paints, well fact that up-to-date there are not reports about measurements assessing this condition, led us evaluate damage caused by organic solvents contained through quantification comet assay, sample 33 individuals taken from 10...
<h3>Objective</h3> Chromosomal instability (CIN) is a hallmark of cancer characterized by cell-to-cell variability in the number or structure chromosomes, frequently observed cell populations and associated with poor prognosis, metastasis, therapeutic resistance. Breast (BC) unstable karyotypes recent reports have indicated that CIN may influence response BC to chemotherapy regimens. However, paradoxical associations between extreme improved outcome been observed. <h3>Methods</h3> This study...
Breast cancer (BC) is the type of with highest incidence and mortality rates in women world. In treatment this neoplasia, several therapies are applied, including radiotherapy, hormonal therapy, chemotherapy, biological therapy. Although most patients respond to these types some over time, develop resistance or eventually relapse. Considering above, future therapeutic concepts BC being directed at individualization therapy escalation based on tumor biology through use gene regard, a new...
Evidence supports a role of 17&-estradiol (E 2 ) in carcinogenesis and the large majority breast carcinomas are dependent on estrogen. The anti-estrogen tamoxifen (TAM) is widely used for both treatment prevention cancer; however, it also carcinogenic human uterus rat liver, highlighting profound complexity its actions. nature E - or TAM-induced chromosomal damage has been explored using relatively high concentrations these agents, only some numerical aberrations breaks have analyzed....