A5091308705- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Salmonella and Campylobacter epidemiology
- Immune Response and Inflammation
- Vibrio bacteria research studies
- Clostridium difficile and Clostridium perfringens research
- Antimicrobial Peptides and Activities
- Bacteriophages and microbial interactions
- Diet and metabolism studies
- Animal Virus Infections Studies
- Gut microbiota and health
- Bacterial Infections and Vaccines
- Nutritional Studies and Diet
- Antibiotic Resistance in Bacteria
- Ethnobotanical and Medicinal Plants Studies
- SARS-CoV-2 and COVID-19 Research
- Inhalation and Respiratory Drug Delivery
- Retinoids in leukemia and cellular processes
- Immunotherapy and Immune Responses
- Moringa oleifera research and applications
- Advanced Drug Delivery Systems
- Studies on Chitinases and Chitosanases
- Ginseng Biological Effects and Applications
- Hepatitis Viruses Studies and Epidemiology
- Pneumonia and Respiratory Infections
University of Missouri
2023-2024
Center for Veterinary Medicine
2024
University of Kansas
2020-2023
National Institute of Cholera and Enteric Diseases
2016-2023
Salmonella Typhi and Paratyphi A are the leading causative agents of enteric fever which cause morbidity mortality worldwide. Currently, there is no combination vaccine could protect infection from both strains. In this paper, we focusing on development a novel bivalent typhoidal Outer Membrane Vesicles (OMVs) based immunogen against fever. We have isolated OMVs also characterized associated antigens. Then immunized adult mice with three doses our newly formulated orally (25 μg/200 μl)....
Infections caused by the opportunistic pathogen Pseudomonas aeruginosa can be difficult to treat due innate and acquired antibiotic resistance this is exacerbated emergence of multi-drug resistant strains. Unfortunately, no licensed vaccine yet exists prevent infections. Here we describe a novel subunit that targets P. type III secretion system (T3SS). This based on antigen PaF (Pa Fusion), fusion T3SS needle tip protein, PcrV, first two translocator proteins, PopB. Additionally, made...
Abstract The opportunistic pathogen Pseudomonas aeruginosa (Pa) causes severe nosocomial infections, especially in immunocompromised individuals and the elderly. Increasing drug resistance, absence of a licensed vaccine increased hospitalizations due to SARS-CoV-2 have made Pa major healthcare risk. To address this, we formulated candidate subunit against (L-PaF), by fusing type III secretion system tip translocator proteins with LTA1 an oil-in-water emulsion (ME). This was mixed TLR4...
Pseudomonas aeruginosa is an opportunistic pathogen responsible for a wide range of infections in humans. In addition to its innate antibiotic resistance, P. very effective acquiring resistance resulting the emergence multi-drug strains and licensed vaccine not yet available. We have previously demonstrated protective efficacy novel antigen PaF (Pa Fusion), fusion type III secretion system (T3SS) needle tip protein, PcrV, first two translocator proteins, PopB. was modified provide...
(Pa) is an opportunistic bacterial pathogen responsible for severe hospital acquired infections in immunocompromised and elderly individuals. Emergence of increasingly drug resistant strains the absence a broad-spectrum prophylactic vaccine against both T3SA
Salmonella enterica , a Gram-negative pathogen, has over 2500 serovars that infect wide range of hosts. In humans, S. causes typhoid or gastroenteritis and is major public health concern. this study, SseB (the tip protein the pathogenicity island 2 type III secretion system) was fused with LTA1 subunit labile-toxin from enterotoxigenic E. coli to make self-adjuvanting antigen L-SseB. Two unique nanoparticle formulations were developed allow multimeric presentation Mice vaccinated these...
Shigellosis (bacillary dysentery) is a severe gastrointestinal infection with global incidence of 90 million cases annually. Despite the severity this disease, there currently no licensed vaccine against shigellosis. Shigella ’s primary virulence factor its type III secretion system (T3SS), which specialized nanomachine used to manipulate host cells. A fusion T3SS injectisome needle tip protein IpaD and translocator IpaB, termed DBF, when admixed mucosal adjuvant double-mutant labile toxin...
Shigella spp. are responsible for bacillary dysentery or shigellosis transmitted via the fecal–oral route, causing significant morbidity and mortality, especially among vulnerable populations. There currently no licensed vaccines. use a type III secretion system (T3SS) to invade host cells. We have shown that L-DBF, recombinant fusion of T3SS needle tip (IpaD) translocator (IpaB) proteins with LTA1 subunit enterotoxigenic E. coli labile toxin, is broadly protective against challenge in mouse...
Diabetes mellitus and diarrhea are becoming increasingly burdensome worldwide, particularly in developing countries such as India. Diabetic patients susceptible to infection with pathogenic bacteria, those causing invasive enteric infections. In this study, we observed changes the pathophysiological features of mice streptozotocin-induced hyperglycemia. our experiments, both hyperglycemic control were infected bacteria-non-typhoidal Salmonella, Shigella flexneri, or Vibrio parahaemolyticus....
Shigellosis is a severe diarrheal disease caused by members of the genus Shigella , with at least 80 million cases and 700,000 deaths annually around world. The type III secretion system (T3SS) primary virulence factor used shigellae, we have previously demonstrated that vaccination T3SS proteins IpaB IpaD, along an IpaD/IpaB fusion protein (DBF), protects mice from infection in lethal pulmonary model. To simplify formulation development DBF vaccine, genetically fused LTA1, active subunit...
With the acquirement of antibiotic resistance, Shigella has resulted in multiple epidemics shigellosis, an infectious diarrheal disease, causing thousands deaths per year. Unfortunately, there are no licensed vaccines, primarily due to low or serotype-specific immunogenicity. Thus, conserved subunit vaccines utilizing recombinant invasion plasmid antigens (Ipa) have been explored as cross-protective vaccine candidates. However, achieving cross-protection against dysenteriae 1, which caused...
Shigellosis has been a menace to society for ages. The absence of an effective vaccine against Shigella, improper sanitation, and unhygienic use food water allow the disease flourish. Shigella can also be transmitted via natural bodies. In good animal model, actual nature pathogenesis transmission remains unclear. Zebrafish larvae have previously described as model pathogenesis. However, larval fish lack mature intestinal microbiota immune system. Here, adult zebrafish was assessed potential...
Background Indian immigration to westernized countries has surged in recent years, with this demographic facing a higher risk of Inflammatory Bowel Disease (IBD) after migration. Studying the gut microbiome is crucial understanding factors contributing IBD, yet Indians remain underrepresented research, Objective This study investigates residing India, immigrants Canada, and Canadians ancestry understand impact westernization on their gut. Given IBD among Indo-Canadians, these results provide...
<title>Abstract</title> <italic><bold>Background: </bold></italic>Indian immigration to westernized countries has surged in recent years, with this demographic facing a higher risk of Inflammatory Bowel Disease (IBD) after migration. Studying the gut microbiome is crucial understanding factors contributing IBD, yet Indians remain underrepresented research, <italic><bold>Objective:</bold></italic><italic> </italic>This study investigates residing India, Indian immigrants Canada, and Canadians...