A5050014917- Tryptophan and brain disorders
- Hepatitis Viruses Studies and Epidemiology
- Bipolar Disorder and Treatment
- Tuberculosis Research and Epidemiology
- Schizophrenia research and treatment
- Viral gastroenteritis research and epidemiology
- HIV/AIDS Research and Interventions
- Gut microbiota and health
- HIV, Drug Use, Sexual Risk
- Hepatitis C virus research
- Liver Disease and Transplantation
- Maternal Mental Health During Pregnancy and Postpartum
- Infectious Diseases and Tuberculosis
- Hepatitis B Virus Studies
- COVID-19 epidemiological studies
- Advanced Chemical Sensor Technologies
- COVID-19 diagnosis using AI
- Liver Diseases and Immunity
- Biosensors and Analytical Detection
- Viral-associated cancers and disorders
- Neuroendocrine regulation and behavior
- HIV Research and Treatment
- Diagnosis and treatment of tuberculosis
- Cytomegalovirus and herpesvirus research
- Veterinary medicine and infectious diseases
Baylor University
2024
University of the Witwatersrand
2017-2023
National Health Laboratory Service
2017-2023
National Institute for Communicable Diseases
2019-2021
Czech Academy of Sciences, Institute of Microbiology
2010
University of Ghana
2010
Komfo Anokye Teaching Hospital
2010
Kwame Nkrumah University of Science and Technology
2010
Kumasi Technical University
2009
University of Bahrain
2008
Abstract Background Human herpesvirus 8 (HHV-8), cytomegalovirus (CMV) and Epstein-Barr virus (EBV) are prevalent in Africa, but less common elsewhere the modes of transmission still subject to debate. Generally, they rarely cause disease immunocompetent host highly oncogenic when associated with immunosuppression. Although high prevalence HHV-8, CMV EBV has been well documented such data sparse from Ghana. Methods Serum samples 3275 HIV-seronegative healthy blood donors 250 HIV-AIDS...
Prisons are known to be high-risk environments for the spread of bloodborne and sexually transmitted infections. Prison officers considered have an intermittent exposure potential infectious diseases on job, however there has been no studies prevalence these infections in prison Ghana.A national multicenter cross-sectional study was undertaken correlates human immunodeficiency virus (HIV), hepatitis B (HBV), C (HCV), syphilis sample inmates from eight ten regional central prisons Ghana. A...
Hepatitis E virus (HEV) is highly endemic in several African countries with high mortality rate among pregnant women. The prevalence of antibodies to HEV Ghana not known. Therefore we evaluated the anti-HEV IgG and IgM women seen between months January May, 2008 at Obstetrics Gynaecology Department, Korle-Bu Teaching Hospital, Accra, Ghana. One hundred fifty-seven provided blood samples for unlinked anonymous testing presence HEV. median age participants was 28.89 ± 5.76 years (range 13–42...
There is no biomarker for diagnosing active tuberculosis in patients with human immunodeficiency virus (HIV) infection. Indoleamine 2, 3-dioxygenase (IDO) an immunoregulatory enzyme that breaks down tryptophan (Trp) to metabolites known as kynurenines (Kyns). We investigated whether IDO activity, measured by the ratio of Kyn Trp, could be used diagnose or predict disease HIV-infected adults.Kyn and Trp concentrations were using ultraperformance liquid chromatography mass spectrometry plasma...
Abstract Background Approximately 1.25 million individuals died from tuberculosis in 2023, part due to ineffective treatment. In patients with paucibacillary tuberculosis, microbiologic confirmation and drug resistance testing via respiratory specimens is challenging; hence, stool increasingly used for confirmation. Targeted next-generation sequencing (tNGS) of DNA may improve detection resistant (DR)-tuberculosis, support receive appropriate treatments. Methods We assessed the ability a...
Several studies have suggested that elevated serum alanine aminotransferase (ALT) and asparte (AST) may be markers of hepatitis E virus (HEV) infection. Thus, individuals with ALT AST ongoing subclinical infection HEV. We estimated the prevalence anti-HEV antibodies levels among persons who work pigs in Accra, Ghana. Three hundred fifty- provided blood samples for unlinked anonymous testing presence to HEV, levels. The median age participants was 32.85 ± 11.38 years (range 15-70 years). HEV...
IntroductionThe World Health Organization has identified the need for a non-sputum-based test capable of detecting active tuberculosis (TB) as priority. The plasma kynurenine-to-tryptophan (K/T) ratio, largely mediated by activity enzyme indoleamine 2,3-dioxygenase, may have potential suitable biomarker TB.MethodWe evaluated commercial enzyme-linked immunosorbent assay (ELISA) in comparison to mass spectrometry measuring K/T ratio. We also used ELISA determine ratio from patients with TB...
Objective: To determine the correlates of hepatitis E virus infection (HEV) in a sample persons who work with pigs.Design: Cross-sectional study.Setting: Three pig farms Greater Accra Region Ghana.Subjects: Persons pigs seen at selected between months January and May 2008.Results: One hundred five voluntarily completed risk-factor questionnaire provided blood samples for unlinked anonymous testing presence antibodies to HEV. The median age participants was 36.5 ± 15.0 years (range 12-65...
For pregnant women living with human immunodeficiency virus (HIV), concurrent active tuberculosis (TB) disease increases the risk of maternal mortality and poor pregnancy outcomes. Plasma indoleamine 2,3-dioxygenase (IDO) activity measured by kynurenine-to-tryptophan (K/T) ratio has been proposed as a blood-based TB biomarker. We investigated whether plasma K/T could be used to diagnose among HIV.
Abstract Indoleamine 2,3‐dioxygenase (IDO) is the rate‐limiting enzyme in conversion of tryptophan to kynurenines, feeding de novo nicotinamide synthesis. IDO orchestrates materno‐foetal tolerance, increasing human reproductive fitness. mediates immune suppression through depletion required by T lymphocytes and other mechanisms. expressed alternatively activated macrophages, suspected play a key role tuberculosis (TB) pathogenesis. Unlike its host, Mycobacterium can synthesize tryptophan,...
Background. In 1995, the hepatitis B vaccine in South Africa was incorporated into childhood expanded programme of immunization. We report on immunity gaps laboratory-based virus (HBV) among patients public facilities Gauteng Province from 1st January 2014 to 31st December 2019. Methodology. analyzed HBV serological data extracted National Health Laboratory Services Central Data Warehouse (NHLS CDW). A descriptive analysis performed for surface antigen (HBsAg), antibodies core (anti-HBc)...
Introduction: South Africa is yet to introduce rubella-containing vaccines (RCV) into its routine immunization schedule. Selecting the target population when introducing RCV should take account ages of susceptible individuals in population. We aimed determine seroprevalence antibodies rubella and characterize immunity gaps among all Africa.Methods: tested for immunoglobulin G (IgG) with a commercial enzyme-linked immunosorbent assay. used residual samples collected from 2016 through 2018 as...
Introduction: Children have paucibacillary tuberculosis and cannot provide expectorated sputum. Invasive specimen collection, by gastric aspiration or sputum induction, has a low diagnostic yield. In this study, we aimed to evaluate the performance additive yield of novel stool-based assay in children diagnosed with sub-Saharan Africa.Methods: We conducted prospective case-control study from October 7, 2020 June 26, 2023 Eswatini, Mozambique, Tanzania. All under 15 years old enrolled as...
Background: The World Health Organization has identified the need for a non-sputum-based test capable of detecting active tuberculosis (TB) as key TB control intervention. Indoleamine 2,3-dioxygenase (IDO) is an enzyme, which converts tryptophan to kynurenines. Recently, IDO activity been proposed blood-based biomarker. We investigated whether plasma activity, measured by kynurenine-to-tryptophan ratio, could be used screen HIV-infected pregnant women disease. Methods and materials: Using...
Coronavirus Disease 2019 (COVID-19) has become a major health problem causing severe acute respiratory illness in humans. With the high case count and mortality rate, broad testing method is required to detect virus infected people, as well less common clinical manifestations of disease. Consequently, demand for resulted depletion commercially available consumables, including recommended swabs viral transport media (VTM) oropharyngeal sampling. To address this issue, we evaluated utility...