A5031826622- Transgenic Plants and Applications
- SARS-CoV-2 and COVID-19 Research
- Toxin Mechanisms and Immunotoxins
- Viral gastroenteritis research and epidemiology
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- dental development and anomalies
- Insect symbiosis and bacterial influences
- Microbial Inactivation Methods
- Invertebrate Immune Response Mechanisms
- Bone and Dental Protein Studies
- Poxvirus research and outbreaks
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- CRISPR and Genetic Engineering
- Biochemical and Structural Characterization
- Neurobiology and Insect Physiology Research
- Herbal Medicine Research Studies
- Periodontal Regeneration and Treatments
- Infections and bacterial resistance
- Antibiotic Resistance in Bacteria
- SARS-CoV-2 detection and testing
- Salmonella and Campylobacter epidemiology
- Bone Metabolism and Diseases
Chulalongkorn University
2017-2023
Pharmac
2020
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is responsible for the ongoing global outbreak of coronavirus disease (COVID-19) which a significant threat to public health. The rapid spread COVID-19 necessitates development cost-effective technology platforms production vaccines, drugs, and protein reagents appropriate diagnosis treatment. In this study, we explored possibility producing receptor binding domain (RBD) SARS-CoV-2 an anti-SARS-CoV monoclonal antibody (mAb) CR3022...
The striking innovation and clinical success of immune checkpoint inhibitors (ICIs) have undoubtedly contributed to a breakthrough in cancer immunotherapy. Generally, ICIs produced mammalian cells requires high investment, production costs, involves time consuming procedures. Recently, the plants are considered as an emerging protein platform due its cost-effectiveness rapidity for recombinant biopharmaceuticals. This study explored potential plant-based system produce anti-human PD-1...
Cytotoxic T lymphocyte-associated protein 4 (CTLA-4) is an immune checkpoint regulator exclusively expressed on cells that obstructs the cell’s effector functions. Ipilimumab (Yervoy®), a CTLA-4 blocking antibody, emerged as notable breakthrough in modern cancer treatment, showing upfront clinical benefits multiple carcinomas. However, exhilarating cost of blockade therapy discouraging and even utmost prominent developing countries. Thereby, affordability care has become point emphasis drug...
The constantly emerging severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) variants of concerns (VOCs) with mutations in the receptor-binding domain (RBD) spread rapidly and has become a public health problem worldwide. Effective vaccines optimized booster vaccination strategies are thus highly required. Here, gene encoding six different RBD (Alpha, Beta, Gamma, Kappa, Delta, Epsilon variants) along Fc fragment human IgG1 (RBD-Fc) was cloned into plant expression vector produced...
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is responsible for the ongoing coronavirus disease (COVID-19) pandemic which characterized by illness and severe pneumonia, currently accounts > 2.5 million deaths worldwide. Recently, diverse mutations in spike protein of SARS-CoV-2 were reported United Kingdom (Alpha) South Africa (Beta) strains raise concerns over potential increase binding affinity towards host cell receptor diminished neutralization capabilities. In order...
SARS-CoV-2 causes devastating impact on the human population and has become a major public health concern. The frequent emergence of variants concern urges development safe efficacious vaccine against variants. We developed candidate Baiya Vax 1, based receptor-binding domain (RBD) by fusing with Fc region IgG. RBD-Fc fusion was produced in Nicotiana benthamiana. Previously, we reported that this plant-produced is effective inducing immune response both mice non-human primates. Here,...
Abstract Checkpoint blockade immunotherapy has revolutionized cancer treatment, with monoclonal antibodies targeting immune checkpoints, yielding promising clinical benefits. However, the advent of resistance to checkpoint inhibitor treatment in trials, developing next-generation potentially increased efficacy is critical. Here, we aimed generate a recombinant bispecific antibody for dual inhibition programmed cell death protein 1/programmed ligand 1 and cytotoxic T-lymphocyte-associated 4...
Inductive molecules are critical components for successful bone tissue engineering. Dentin matrix protein-1 (DMP1), a non-collagenous protein in the matrix, has been shown to play roles osteogenic differentiation and phosphate homeostasis. This study aimed produce recombinant human dentin (hDMP1) Nicotiana benthamiana investigated ability of this plant-produced DMP1 induce osteogenesis periodontal ligament stem cells (hPDLSCs). The hDMP1 gene was cloned into geminiviral vector transient...
Cobra (Naja kaouthia) venom contains many toxins including α-neurotoxin (αNTX) and phospholipase A2 (PLA2), which can cause neurodegeneration, respiratory failure, even death. The traditional antivenom derived from animal serum faces challenges limitations. Heavy-chain-only antibodies (HCAb), fusing VHH with human IgG Fc region, offer advantages in tissue penetration, antigen binding, extended half-life. This research involved the construction transient expression of two types VHH-FC are...
Since the outbreak of coronavirus disease (COVID) pandemic in 2019, development effective vaccines to combat infection has been accelerated. With recent emergence highly transmissible severe acute respiratory syndrome 2 (SARS-CoV-2) variants concern (VOC), there are concerns regarding immune escape from vaccine-induced immunity. Hence an vaccine against VOC with a potent response is required. Our previous study confirmed that two doses plant-produced receptor-binding domain (RBD) SARS-CoV-2...
The study aimed to produce recombinant human dentin matrix protein 1 (DMP1) and test, whether the DMP1 produced in Escherichia coli possesses functional activity. A gene construction comprising a encoding for with polyhistidine sequence at its C-terminus was created using pET22b plasmid expressed E. coli. optimization of cultivation conditions has enabled induction expression 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) production 37 °C 6 h. purified Ni affinity chromatography....
Recently we have generated recombinant human osteopontin (rhOPN) using a plant platform (Nicotiana benthamiana) and demonstrated, when coated on culture plates, its osteogenic induction capacity of periodontal ligament (PDL) cells. The aim this study is to elucidate the molecular mechanism underlying rhOPN-induced differentiation PDL cells.Full length rhOPN (FL-OPN) three constructs OPN containing integrin binding domain (N142), calcium (C122) mutated calcium-binding (C122δ) were from N....
SARS-CoV-2 is responsible for the ongoing COVID-19 pandemic. The virus spreads rapidly with a high transmission rate among humans, and hence management has been challenging owing to finding specific therapies or vaccinations. Hence, an effective, low-cost vaccine urgently required. In this study, immunogenicity of plant-produced S1 subunit protein was examined in order assess it as potential candidate SARS-CoV-2. S1-Fc fusion transiently produced Nicotiana benthamiana. Within four days...
Abstract Severe acute respiratory syndrome coronavirus-2 is responsible for an ongoing global outbreak of coronavirus disease (COVID-19) and represents a significant public health threat. The rapid spread COVID-19 necessitates the development cost-effective technology platforms production diagnostic reagents/biopharmaceuticals COVID-19. We explored possibility producing anti-SARS-CoV monoclonal antibody (mAb) CR3022 receptor binding domain (RBD) SARS-CoV-2 in Nicotiana benthamiana . Both RBD...