A5063234606- Mosquito-borne diseases and control
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Malaria Research and Control
- SARS-CoV-2 detection and testing
- COVID-19 epidemiological studies
- Vaccine Coverage and Hesitancy
- Primate Behavior and Ecology
- Virology and Viral Diseases
- Biosensors and Analytical Detection
- Long-Term Effects of COVID-19
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Viral Infections and Outbreaks Research
- Animal Virus Infections Studies
- Influenza Virus Research Studies
- Zoonotic diseases and public health
- Respiratory viral infections research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Vector-borne infectious diseases
- Heparin-Induced Thrombocytopenia and Thrombosis
- Toxin Mechanisms and Immunotoxins
- HIV Research and Treatment
Armed Forces Research Institute of Medical Science
2015-2025
Ministry of Public Health
2021
Keelung Chang Gung Memorial Hospital
2021
Thai Red Cross Society
2021
Chulalongkorn University
2021
Mahidol University
2015
Khon Kaen University
2011-2012
Abstract Background Travel to Southeast Asia increases the likelihood of acquiring mosquito-borne Flavivirus infections such as dengue (DENV), Japanese encephalitis (JEV) and Zika viruses (ZIKV). Expatriates are long-term travellers who have a higher risk illness at their destination country. The purpose this study was evaluate seroprevalence DENV, JEV ZIKV determinants contributing seropositivity among expatriates living in Thailand. Methods A cross-sectional performed from December 2017...
Chikungunya virus (CHIKV) infections are distributed across the globe, causing significant and, often, lasting morbidity. CHIKV vaccines in development, but their evaluation is limited by unpredictability of transmission, which classically manifests as explosive epidemics separated variable interepidemic periods. A passive surveillance study for undifferentiated febrile illness was established southern Thailand 2012 and ongoing. Among 1466 individuals with acute convalescent specimens, 398...
Virus-like particles (VLPs) are highly immunogenic and versatile subunit vaccines composed of multimeric viral proteins that mimic the whole virus but lack genetic material. Due to infectivity, VLPs being developed as safe effective against various infectious diseases. In this study, we generated a chimeric VLP-based COVID-19 vaccine stably produced by HEK293T cells. The contain influenza A matrix (M1) SARS-CoV-2 Wuhan strain spike (S) with deletion polybasic furin cleavage motif replacement...
Abstract Background Zika virus (ZIKV) is a mosquito-transmitted flavivirus that affects many regions of the world. Infection, in utero, causes microcephaly and later developmental neurologic impairments. The impact ZIKV infection on neurocognition adults has not been well described. objective study was to assess neurocognitive adult rhesus macaques. Methods Neurocognitive assessments were performed using Cambridge Neuropsychological Test Automated Battery (CANTAB) via touch screen modified...
Dengue viruses (DENVs) are among the most rapidly and efficiently spreading arboviruses. WHO recently estimated that about half of world's population is now at risk for DENV infection. There no specific treatment or vaccine available to treat prevent infections. Here, we report development a novel dengue nanovaccine (DNV) composed UV-inactivated DENV-2 (UVI-DENV) Mycobacterium bovis Bacillus Calmette-Guerin cell wall components (BCG-CWCs) loaded into chitosan nanoparticles (CS-NPs). CS-NPs...
Dengue virus (DENV), a member of the Flaviviridae family, can be transmitted to humans through bite infected Aedes mosquitoes. The incidence dengue has increased worldwide over past few decades. Inadequate vector control, changing global ecology, urbanization, and faster travel are factors enhancing rapid spread its vector. In absence specific antiviral treatments, search for safe effective vaccine grows more imperative. Many strategies have been utilized develop vaccines. Here, we...
Abstract The rapid emergence of SARS-CoV-2 variants with high severity and transmutability adds further urgency for multiplex molecular testing to identify the variants. A nucleotide matrix-assisted laser-desorption-ionization time-of-flight mass spectrophotometry (MALDI-TOF MS)-based assay was developed (called point mutation array, PMA) four major concern (VOCs) including Alpha, Beta, Delta, Omicron (namely PMA-ABDO) differentiate subvariant PMA-Omicron). PMA-ABDO PMA-Omicron consist 24 28...
Individuals infected with dengue virus (DENV) often show no symptoms, which raises the risk of DENV transfusion transmission (TT-DENV) in areas where is prevalent. This study aimed to determine evidence infection blood donors from different geographic regions Thailand. A cross-sectional was conducted on donor samples collected Thai Red Cross National Blood Center and four regional centers between March September 2020. Screening for nonstructural protein 1 (NS1), anti-DENV immunoglobulin G...
In vitro determination of severe acute respiratory syndrome coronavirus 2 neutralizing antibodies induced in serum samples from recipients the CoronaVac vaccine showed a short protection period against original virus strain and limited variants concern. These data provide support for boosters, especially concern circulate.
We examined SARS-CoV-2 anti-spike 1 IgG antibody levels following COVID-19 vaccination (AstraZeneca [AZ], Sinovac [SV], Pfizer-BioNTech [PZ]) among Thai healthcare providers.
Background The dengue vaccine (Dengvaxia) is only recommended for individuals with prior infection (PDI). This study aimed to perform a serosurvey inform decision-making introduction and identify appropriate target populations. We also evaluated the performance of serological tests using plaque reduction neutralization test (PRNT) as reference in identifying PDI determine suitability pre-vaccination screening. Methods enrolled 115 healthy between 10 22 years age living Ratchaburi province...
The pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently the biggest healthcare issue worldwide. This study aimed to develop a monoclonal antibody against SARS-CoV-2 from B cells recovered COVID-19 patients, which might have beneficial therapeutic purposes for patients. We successfully generated human antibodies (hmAbs) receptor binding domain (RBD) protein using developed hybridoma technology. isolated hmAbs RBD (wild-type) showed high activity and...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of disease 2019 (COVID-19). RT-PCR detection viral RNA represents gold standard method for diagnosis COVID-19. However, multiple diagnostic tests are needed and assessing immunity during COVID-19 outbreak. Here, we developed in-house anti-RBD IgG IgA enzyme-linked immunosorbent assays (ELISAs) using a well-defined serum sample panel screening identification human SARS-CoV-2 infection. We found that our...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is found in regions where dengue (DENV) and chikungunya (CHIKV) viruses are endemic. Any serological cross-reactivity between DENV, CHIKV, SARS-CoV-2 significant as it could lead to misdiagnosis, increased severity, or cross-protection. This study examined the potential of anti-DENV CHIKV antibodies with using convalescent-phase samples collected before pandemic. These included healthy, normal human (NHS, n = 6), CHIKV-positive (n...
Dengue is a re-emerging global public health problem, the most common arbovirus causing human disease in world, and major cause of hospitalization endemic countries significant economic burden. Data were analyzed from passive surveillance hospital-attended dengue cases 2002 to 2018 at Phramongkutklao Hospital (PMKH) located Bangkok, Thailand, Kamphaeng Phet Provincial (KPPH) lower northern region Thailand. At PMKH, serotype 1 proved be strain virus, whereas KPPH, serotypes 1, 2, 3 strains...
Background Thailand was the first country outside China to report SARS-CoV-2 infected cases. Since detection of imported case on January 12 th , 2020 time this written, experienced two waves community outbreaks (March-April and December 2020-March 2021). We examined prevalence seropositivity among healthcare providers (HCPs) in four hospitals approximately one year after detected Thailand. By March 2021, these have treated a total 709 coronavirus disease 2019 (COVID-19) patients. Methods...
Here, we describe the development of in-house anti-Zika virus (ZIKV) IgM antibody capture ELISA (in-house ZIKV ELISA) for detection and diagnosis acute infections. We compared assay performance against two commercial kits, Euroimmun InBios 2.0 ELISA. tested assays' ability to detect anti-ZIKV using a well-defined serum sample panel. This panel included 80 negative samples (20 negative, 20 found be primary dengue [DENV][ infections, secondary DENV Japanese encephalitis [JEV] infections) 67...
We determined the levels of neutralizing antibodies against SARS-CoV-2 ancestral strain, Delta and Omicron variants concern (VOCs), in 125 healthcare workers who received CoronaVac as their primary vaccination later either a single ChAdOx1 or combi-nation two consecutive boosters using doses BNT162b2 second boosters, respectively, BNT162b2. The titers 12 weeks after were inadequate to neutralize all strains. After booster, neutralization at Day 30 varied significantly, with only small...
We determined the levels of neutralizing antibodies against SARS-CoV-2 ancestral strain, Delta and Omicron variants concern (VOCs) in 125 healthcare workers, who received Coro-naVac as their primary vaccination later either a single ChAdOx1 or combination two consecutive boosters using doses BNT162b2 second boosters, respectively, 2 BNT162b2. The titers 12 weeks after were inadequate to neutralize all strains. After boost-er, neutralization at Day 30 varied significantly, only small...
Abstract The development of a safe and efficacious vaccine that provides immunity against all four dengue virus serotypes is priority, significant challenge for has been defining measuring serotype-specific outcomes correlates protection. plaque reduction neutralisation test (PRNT) the gold standard assay antibodies, but this cannot differentiate homotypic heterotypic antibodies characterising infection history challenging. To address this, we present an analysis pre- post-infection antibody...
Zika virus (ZIKV) outbreaks occur sporadically in tropical and subtropical regions. At present, there are no licensed vaccines or specific treatments available for ZIKV. Ivermectin is approved use humans as an antiparasitic drug. In this study, we conducted vitro cell culture vivo experiments rhesus macaque hosts Aedes aegypti vectors to investigate the potential of ivermectin inhibitor ZIKV infection. LLC-MK2 mammalian cells, inhibited growth with 50% inhibitory concentration (IC50) values...
Uncovering rates at which susceptible individuals become infected with a pathogen, i.e., the force of infection (FOI), is essential for assessing transmission risk and reconstructing distribution immunity in population. For dengue, exposure susceptibility statuses from measured FOI particular significance as prior strong factor severe disease. can be via many study designs. Longitudinal serology considered gold standard measurements, they directly track transition seronegative to...