A5030130262- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Molecular Biology Techniques and Applications
- Neurobiology and Insect Physiology Research
- Invertebrate Immune Response Mechanisms
- Erythrocyte Function and Pathophysiology
- Neonatal Health and Biochemistry
- Genomics and Chromatin Dynamics
- Thyroid Disorders and Treatments
- Cancer-related molecular mechanisms research
- Animal Behavior and Reproduction
- Amino Acid Enzymes and Metabolism
University of Oxford
2020-2025
Constructor University
2022
Ludwig-Maximilians-Universität München
2018
Visualizing Xist RNA dynamics The noncoding Xist, which controls the process of X chromosome inactivation in mammals, accumulates and spreads over from it is transcribed. underlying basis for this unusual behavior poorly understood. Using a new imaging approach called RNA-SPLIT time-resolved analysis molecules at super-resolution, Rodermund et al. analyzed fundamental parameters normal cells after perturbation factors implicated function. authors provide insights into localization...
X chromosome inactivation (XCI) is mediated by the non-coding RNA Xist, which directs chromatin modification and gene silencing in cis. The binding protein SPEN associated corepressors have a central role Xist-mediated silencing. Other factors, notably Polycomb system, been reported to function downstream of SPEN. In recent work, we found that has an additional correct localization Xist cis, indicating its contribution chromatin-mediated needs be reappraised. Making use...
Abstract X chromosome inactivation (XCI) in mammals is orchestrated by the non-coding RNA Xist which together with specific interacting proteins, functions cis to silence an entire chromosome. Defined sites on carry N 6 -methyladenosine (m A) modification, and perturbation of m A writer complex has been found abrogate Xist-mediated gene-silencing. However, relative contribution its mechanism action remain unclear. Here we investigate role XCI applying rapid degron-mediated depletion METTL3,...
Pigmentation is a diverse and ecologically relevant trait in insects. Pigment formation has been studied extensively at the genetic biochemical levels. The temporality of pigment during animal development, however, more elusive. Here, we examine this temporality, focusing on yellow, gene involved black melanin. We generated protein-tagged yellow allele fruit fly Drosophila melanogaster, which allowed us to precisely describe Yellow expression pattern tissue cellular levels throughout...
Abstract Xist RNA directs the process of X-chromosome inactivation in mammals by spreading cis along chromosome from which it is transcribed and recruiting chromatin modifiers to silence gene transcription. To elucidate mechanisms -confinement, we established a sequential dual color labeling, super-resolution imaging approach trace individual molecules over time, enabling us define fundamental parameters spreading. We demonstrate feedback mechanism linking synthesis degradation, an...
Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)
Proteolytic cleavage of thyroglobulin (Tg) for thyroid hormone (TH) liberation is followed by TH release from follicles into the circulation, enabled transporters. The existence a functional link between Tg-processing cathepsin proteases and transporters has been shown to be independent hypothalamus-pituitary-thyroid axis. Thus, lack K, combined with genetic defects in Mct8 Mct10, that Ctsk-/-/Mct8-/y/Mct10-/- genotype, results persistent Tg proteolysis due autophagy induction. Because amino...
Summary X chromosome inactivation (XCI) is mediated by the non-coding RNA Xist which directs chromatin modification and gene silencing in cis . The binding protein SPEN associated corepressors have a central role Xist-mediated silencing. Other factors, notably Polycomb system, been reported to function downstream of SPEN. In recent work we found that has an additional correct localisation , indicating its contribution chromatin-mediated needs be reappraised. Making use separation-of-function...