A5074050758- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Protist diversity and phylogeny
- Genomics and Phylogenetic Studies
- Marine Biology and Ecology Research
- Environmental DNA in Biodiversity Studies
- Methane Hydrates and Related Phenomena
- Bacteriophages and microbial interactions
- Arctic and Antarctic ice dynamics
- Aquaculture disease management and microbiota
- Marine Bivalve and Aquaculture Studies
- Aquatic Ecosystems and Phytoplankton Dynamics
- Marine and coastal plant biology
- Algal biology and biofuel production
- Isotope Analysis in Ecology
- Biosensors and Analytical Detection
- Marine Ecology and Invasive Species
- Parasitic Infections and Diagnostics
- Climate change and permafrost
- Freshwater macroinvertebrate diversity and ecology
- Coastal wetland ecosystem dynamics
- Microbial Fuel Cells and Bioremediation
- Diatoms and Algae Research
- Plant and Fungal Interactions Research
- Aquatic Invertebrate Ecology and Behavior
National Marine Fisheries Research Institute
2015-2025
Czech Academy of Sciences
2016-2024
Institute of Hydrobiology, Biology Centre, Academy of Sciences of the Czech Republic
2024
Czech Academy of Sciences, Institute of Microbiology
2016-2023
Instytut Oceanologii Polskiej Akademii Nauk
2008-2009
Polish Academy of Sciences
2008-2009
Ongoing climate warming is causing a dramatic loss of sea ice in the Arctic Ocean, and it projected that Ocean will become seasonally ice-free by 2040. Many studies local food webs now exist, with this review paper we aim to synthesize these into large-scale assessment current status knowledge on structure various marine their response change, sea-ice retreat particular. Key drivers ecosystem change potential consequences for functioning are identified along gradient, special emphasis...
High-throughput sequencing (HTS) of gene amplicons is a preferred method assessing microbial community composition, because it rapidly provides information from large number samples at high taxonomic resolution and low costs. However, mock studies show that HTS data poorly reflect the actual relative abundances individual phylotypes, casting doubt on reliability subsequent statistical analysis interpretation. We investigated how accurately variability bacterial eukaryotic composition their...
Gemmatimonadota is a diverse bacterial phylum commonly found in environments such as soils, rhizospheres, fresh waters, and sediments. So far, the contains just six cultured species (five of them sequenced), which limits our understanding their diversity metabolism. Therefore, we analyzed over 400 metagenome-assembled genomes (MAGs) 5 culture-derived representing from various aquatic environments, hydrothermal vents, sediments, host-associated (with marine sponges coral) species. The...
Abstract Due to climate change the pathogenic bacterium Vibrio vulnificus proliferates along brackish coastlines, posing risks public health, tourism, and aquaculture. Here we investigated previously suggested regulation measures reduce prevalence of V. , locally through seagrass regionally reduction eutrophication consequential formation algal blooms. Field samples collected in summer 2021 covered salinity gradients Baltic Sea, one largest areas worldwide. Physico-, biological-...
We investigated the temporal dynamics and trophic role of different nanoflagellates in surface waters Gulf Gdańsk (Baltic Sea) between April October 2007. Two 18S rRNA gene clone libraries were constructed from samples collected spring summer, weekly changes abundances five phylogenetic groups studied by fluorescence situ hybridization with newly designed probes. Stramenopiles affiliated MAST-6 Pedinellales most numerous but rare summer. Both formed short-lived blooms during a sudden drop...
The Arctic system is one of the regions most influenced by ongoing global climate change, but there are still critical gaps in our knowledge regarding a substantial number biological processes. This especially true for processes taking place during winter also seasonal processes, such as dynamics intra-annual meroplankton occurrence. Here, we report on 1-year study variability from fjordic Archipelago Svalbard. combines an examination phytoplankton, zooplankton, and hard bottom benthic...
The genus Limnohabitans (Comamonadaceae, Betaproteobacteria) is a common and highly active component of freshwater bacterioplanktonic communities. To date, the has been considered to contain only heterotrophic species. In this study, we detected photosynthesis genes pufLM bchY in 28 46 strains from three lineages. pufM sequences obtained are very closely related environmental various habitats, indicating ubiquity potential importance photoheterotrophic nature. Additionally, sequenced...
We investigated the spatial and inter-annual variations in planktonic protist community taxonomic composition with respect to size trophic structure West Spitsbergen waters during 2007, 2009 2010 summers. The communities were studied along a longitudinal transect from Kongsfjorden through shelf open sea waters. Our investigation was conducted on Atlantic water inflow, which varied between years. observed weakening trend northward advection of warm, Eastern branch Fram Strait, confirmed by...
Gemmatimonas phototrophica is, so far, the only described phototrophic species of bacterial phylum Gemmatimonadetes. Its cells contain a unique type photosynthetic complex with reaction center surrounded by double ring antenna, however they can also grow in dark using organic carbon substrates. photosynthesis genes were received via horizontal gene transfer from Proteobacteria. This raises two questions; how horizontally transferred apparatus has integrated into cellular machinery, and much...
Aerobic anoxygenic phototrophic (AAP) bacteria are heterotrophic that supply their metabolism with light energy harvested by bacteriochlorophyll-a-containing reaction centers. Despite substantial contribution to bacterial biomass, microbial food webs, and carbon cycle, phenology in freshwater lakes remains unknown. Hence, we investigated seasonal variations of AAP abundance community composition biweekly across 3 years a temperate, meso-oligotrophic lake. displayed clear trend spring maximum...
Abstract Molecular identification of micro- and macroorganisms based on nuclear markers has revolutionized our understanding their taxonomy, phylogeny ecology. Today, research the diversity eukaryotes in global ecosystems heavily relies ribosomal RNA (rRNA) markers. Here, we present community-curated reference database EUKARYOME for 18S rRNA, internal transcribed spacer (ITS) 28S rRNA all eukaryotes, including metazoans (animals), protists, fungi plants. It is particularly useful arbuscular...
Abstract Background Zostera marina is an important ecosystem engineer influencing shallow water environments and possibly shaping the microbiota in surrounding sediments water. Z. typically found marine systems, but it can also proliferate under brackish conditions. Changes salinity generally have a strong impact on biota, especially at salty divide between 6 9. To better understand of interaction sediment microbiota, we investigated effects meadows across range 6–15 Baltic Sea during summer...
In aquatic microbial food webs, ciliates represent an important trophic link in the energy transfer from prokaryotes, algae, and heterotrophic nanoflagellates (HNF) to higher levels. However, role of abundant small (< 20 µm) is not clearly understood. To unveil their linkages, we conducted two experiments manipulating both top-down bottom-up controlling factors, thus modulating cascading bacterial prey availability for protists during contrasting spring summer seasons with samples...
Abstract Eutrophication in the Baltic Sea has caused an imbalance inorganic nitrogen (N) to phosphorus (P) ratio, leaving excess phosphate (PO 4 ) after phytoplankton spring bloom that terminates N depletion. Using monitoring data, we demonstrated PO concentration continued increase outermost Gulf of Finland during past decades. We further investigated fate such a two‐week mesocosm (1.2 m 3 experiment. The starting was 0.66 μ M, and treatments included non‐treated control (control), nitrate...
Members of the bacterial phylum
Abstract Lakes are a significant component of the global carbon cycle. Respiration exceeds net primary production in most freshwater lakes, making them source CO2 to atmosphere. Driven by heterotrophic microorganisms, respiration is assumed be unaffected light, thus it measured dark. However, photoheterotrophs, such as aerobic anoxygenic photoheterotrophic (AAP) bacteria that produce ATP via photochemical reactions, substantially reduce light. They an abundant and active bacterioplankton,...
Metabolic coupling between phytoplankton and bacteria determines the fate of dissolved organic carbon in aquatic environments, yet how changes rate primary production affect bacterial activity community composition remains understudied. Here, we experimentally limited either by lowering light intensity or adding a photosynthesis inhibitor. The induced decrease had greater influence on respiration than growth rate, especially at an optimal intensity. This suggests that drive activity, but...
Abstract Eutrophication in the Baltic Sea has caused an imbalance inorganic nitrogen (N) to phosphorus (P) ratio, leaving excess phosphate (PO 4 ) after phytoplankton spring bloom that terminates N-depletion. Using monitoring data, we demonstrated PO concentration continued increase outermost Gulf of Finland during past decades. We further investigated fate such a two-week mesocosm (1.2 m 3 experiment. The starting was 0.66 µM, and treatments included non-treated control (control), nitrate...
Abstract Photosynthetic cryptophytes are ubiquitous protists that major participants in the freshwater phytoplankton bloom at onset of spring. Mortality due to change environmental conditions and grazing have been recognized as key factors contributing collapse. In contrast, role viral outbreaks terminating blooms remains unknown from freshwaters. Here, we isolated characterized a cryptophyte virus annual collapse natural spring population. This isolate is also representative for clade...