A5070251922- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Photosynthetic Processes and Mechanisms
- Genomics and Phylogenetic Studies
- Protist diversity and phylogeny
- Algal biology and biofuel production
- Photoreceptor and optogenetics research
- Light effects on plants
- Microbial Fuel Cells and Bioremediation
- Spectroscopy and Quantum Chemical Studies
- Marine Biology and Ecology Research
- Metabolomics and Mass Spectrometry Studies
- Isotope Analysis in Ecology
- Wastewater Treatment and Nitrogen Removal
- Mitochondrial Function and Pathology
- Electrochemical sensors and biosensors
- Aquatic Ecosystems and Phytoplankton Dynamics
- Ocean Acidification Effects and Responses
- Bacterial Genetics and Biotechnology
- Methane Hydrates and Related Phenomena
- Analytical Chemistry and Sensors
- Biocrusts and Microbial Ecology
- dental development and anomalies
- Antioxidant Activity and Oxidative Stress
- Oral microbiology and periodontitis research
Czech Academy of Sciences, Institute of Microbiology
2016-2025
University of South Bohemia in České Budějovice
2014-2024
Czech Academy of Sciences
2000-2024
Sewanee: The University of the South
1999-2023
Ecosystem (Spain)
2023
Institute of Microbiology
2015-2019
Rybářství Třeboň (Czechia)
2009
Rutgers, The State University of New Jersey
2001-2006
University of New Brunswick
2004
Moss Landing Marine Laboratories
2004
The availability of iron is known to exert a controlling influence on biological productivity in surface waters over large areas the ocean and may have been an important factor variation concentration atmospheric carbon dioxide glacial cycles. effect Southern Ocean particularly because its area abundant nitrate, yet iron-enhanced growth phytoplankton be differentially expressed between with high silicic acid south low north, where diatom limited by both iron. Two mesoscale experiments,...
The vertical distribution of bacteriochlorophyll a , the numbers infrared fluorescent cells, and variable fluorescence signal at 880 nanometers wavelength, all indicate that photosynthetically competent anoxygenic phototrophic bacteria are abundant in upper open ocean comprise least 11% total microbial community. These organisms facultative photoheterotrophs, metabolizing organic carbon when available, but capable photosynthetic light utilization is scarce. They globally distributed euphotic...
Significance Photosynthesis is one of the most fundamental biological processes on Earth. To date, species capable performing (bacterio)chlorophyll-based phototrophy have been reported in six bacterial phyla. Here we report a phototrophic bacterium belonging to rare and understudied phylum Gemmatimonadetes. This strain, isolated from freshwater lake Gobi Desert, contains fully functional photosynthetic reaction centers. Its photosynthesis genes appear originate an ancient horizontal gene...
A novel group of aerobic anoxygenic phototrophic bacteria was isolated from marine dinoflagellates, and two strains were characterized in detail. Cells Gram-negative cocci or ovoid rods motile by means a single, polarly inserted flagellum. They obligate aerobes requiring 1–7 % salinity. The optimal pH range for growth 6·5–9·0 the temperature optimum 33 °C. contained bacteriochlorophyll spheroidenone as only carotenoid. vivo absorption spectrum displayed maxima infrared region at 804 868 nm....
Upon phosphorus (P) deficiency, marine phytoplankton reduce their requirements for P by replacing membrane phospholipids with alternative non-phosphorus lipids. It was very recently demonstrated that a SAR11 isolate also shares this capability when phosphate starved in culture. Yet, the extent to which process occurs other heterotrophic bacteria and natural environment is unknown. Here, we demonstrate substitution of variety lipids conserved response deficiency among phylogenetically diverse...
The bacterial phylum Gemmatimonadetes contains members capable of performing bacteriochlorophyll-based phototrophy (chlorophototrophy). However, only one strain chlorophototrophic bacteria (CGB) has been isolated to date, hampering our further understanding their photoheterotrophic lifestyle and the evolution in CGB. By combining a culturomics strategy with rapid screening technique for chlorophototrophs, we report isolation new member CGB, Gemmatimonas (G.) groenlandica sp. nov., from...
Gemmatimonadota is a diverse bacterial phylum commonly found in environments such as soils, rhizospheres, fresh waters, and sediments. So far, the contains just six cultured species (five of them sequenced), which limits our understanding their diversity metabolism. Therefore, we analyzed over 400 metagenome-assembled genomes (MAGs) 5 culture-derived representing from various aquatic environments, hydrothermal vents, sediments, host-associated (with marine sponges coral) species. The...
ABSTRACT Growth is one of the basic attributes any living organism. Surprisingly, growth rates marine bacterioplankton are only poorly known. Current data suggest that bacteria grow relatively slowly, having generation times several days. However, some bacterial groups, such as aerobic anoxygenic phototrophic (AAP) bacteria, have been shown to much faster. Two manipulation experiments, in which grazing, viruses, and resource competition were reduced, conducted coastal Mediterranean Sea...
Summary We analysed bacteriochlorophyll diel changes to assess growth rates of aerobic anoxygenic phototrophs in the euphotic zone across Atlantic Ocean. The survey performed during Meridional Transect cruise 16 has shown that North Gyre cycles at 0.91–1.08 day −1 and South 0.72–0.89 . In contrast, more productive equatorial region it cycled up 2.13 These results suggest bacteriochlorophyll‐containing bacteria oligotrophic gyres grow about one division per regions three divisions day. This...
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 53:21-38 (2008) - DOI: https://doi.org/10.3354/ame01230 Special: 'Progress and Perspectives in Ecology: Highlights of SAME 10, Faro, Portugal, September 2007' Towards a better understanding microbial carbon flux sea* Josep M. Gasol1,**, Jarone Pinhassi2,**, Laura Alonso-Sáez3, Hugh Ducklow4, Gerhard J. Herndl5, Michal...
The aerial surface of plants, the phyllosphere, is colonized by numerous bacteria displaying diverse metabolic properties that enable their survival in this specific habitat. Recently, we reported on presence microbial rhodopsin harbouring top leaf surfaces. Here, report additional bacterial populations capable harvesting light as a means supplementing requirements. An analysis six phyllosphere metagenomes revealed community anoxygenic phototrophic bacteria, including previously...
A red-pigmented, bacteriochlorophyll (BChl) a -producing strain, AP64 T , was isolated previously from the freshwater Swan Lake located in western Gobi Desert. Based on its 16S rRNA gene sequence identity (96.1 %) to type strain Gemmatimonas aurantiaca T-27 new isolate tentatively classified as member of bacterial phylum Gemmatimonadetes . Here, we report formal description and polyphasic characterization. Strain grew best agar media under 9.8–15.2 % atmospheric oxygen. The cells were rods,...
ABSTRACT Aerobic anoxygenic phototrophs contain photosynthetic reaction centers composed of bacteriochlorophyll. These organisms are photoheterotrophs, as they require organic carbon substrates for their growth whereas light-derived energy has only an auxiliary function. To establish the contribution light to metabolism, we grew phototrophic strain Erythrobacter sp. NAP1 in a carbon-limited chemostat regimen on defined sources (glutamate, pyruvate, acetate, and glucose) under conditions...
Abstract The capacity for anoxygenic photosynthesis is scattered throughout the phylogeny of Proteobacteria. Their genes are typically located in a so-called gene cluster (PGC). It unclear (i) whether phototrophy an ancestral trait that was frequently lost or (ii) it acquired later by horizontal transfer. We investigated evolution 105 genome-sequenced Rhodobacteraceae and provide first unequivocal evidence transfer PGC. 33 concatenated core PGC formed robust phylogenetic tree comparison with...
Summary Gemmatimonadetes represents a poorly understood bacterial phylum with only handful of cultured species. Recently, one its few representatives, G emmatimonas phototrophica , was found to contain purple photosynthetic reaction centres. However, almost nothing is known about the environmental distribution phototrophic emmatimonadetes bacteria. To fill this gap, we took advantage fast‐growing public metagenomic databases and performed an extensive survey metagenomes deposited into NCBI...
Abstract A biosensor for the detection of triazine‐ and phenylurea‐type herbicides was constructed using isolated Photosystem II (PS II) complexes as a biosensing element. PSII from thermophilic cyanobacterium Synechococcus elongatus immobilized on surface screen‐printed sensor composed graphite working electrode Ag/AgCl reference deposited polymeric substrate. The mounted in flow microcell with illumination. principle based fact that selectively block electron transport activity...
The presence of aerobic anoxygenic phototrophs (AAPs) was recently reported from various marine environments; however, there is little information regarding their distribution in fresh waters. We surveyed a number freshwater systems central Europe, by infra-red fluorometry, epifluorescence microscopy, fluorescence emission spectroscopy and pigment analyses. AAPs were found to be abundant several oligotrophic mesotrophic lakes (50-400 ng bacteriochlorophyll l(-1), 10-80% bacterial biomass),...
Background Aerobic anoxygenic photototrophic (AAP) bacteria represent an important group of marine microorganisms inhabiting the euphotic zone ocean. They harvest light using bacteriochlorophyll (BChl) a and are thought to be players in carbon cycling Methodology/Principal Findings phototrophic part microbial communities. Their photosynthetic apparatus is encoded by number genes organized so-called gene cluster (PGC). In this study, organization PGCs was analyzed ten AAP species belonging...
The genus Limnohabitans (Comamonadaceae, Betaproteobacteria) is a common and highly active component of freshwater bacterioplanktonic communities. To date, the has been considered to contain only heterotrophic species. In this study, we detected photosynthesis genes pufLM bchY in 28 46 strains from three lineages. pufM sequences obtained are very closely related environmental various habitats, indicating ubiquity potential importance photoheterotrophic nature. Additionally, sequenced...