A5062696176- RNA modifications and cancer
- Cancer-related gene regulation
- Cancer Cells and Metastasis
- Algal biology and biofuel production
- Carbohydrate Chemistry and Synthesis
- RNA Interference and Gene Delivery
- Synthesis of Organic Compounds
- Cancer Immunotherapy and Biomarkers
- Mesenchymal stem cell research
- Peptidase Inhibition and Analysis
- Analytical Chemistry and Chromatography
- Epigenetics and DNA Methylation
- Cancer Research and Treatments
- Animal Genetics and Reproduction
- Inflammatory mediators and NSAID effects
- Pancreatic and Hepatic Oncology Research
- Seaweed-derived Bioactive Compounds
- CRISPR and Genetic Engineering
- Radiopharmaceutical Chemistry and Applications
- Nanoplatforms for cancer theranostics
- Immune cells in cancer
- Chemical Synthesis and Reactions
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Lung Cancer Treatments and Mutations
- Angiogenesis and VEGF in Cancer
Kansas State University
2016-2025
Manhattan High School
2015
University of Tsukuba
1998-2009
Yokohama National University
2002
Kobe Steel (Japan)
1996
Shinshu University
1993
Showa Denko (Japan)
1970-1985
Kyorin University
1971-1972
Kyorin (United States)
1972
National Cancer Research Institute
1972
Human and rat umbilical cord matrix mesenchymal stem cells (UCMSC) possess the ability to control growth of breast carcinoma cells. Comparative analyses two types UCMSC suggest that UCMSC-dependent regulation is significantly stronger than human UCMSC. Their different tumoricidal abilities were clarified by analyzing gene expression profiles in Microarray analysis revealed differential between untreated naïve those co-cultured with species-matched The screened 17 differentially expressed...
The newly purified extracellular polysaccharides (exopolysaccharides) from Parachlorella kessleri (PCEPS) were evaluated on their antitumor and immunomodulatory effects in cell culture mouse colon carcinoma peritoneal dissemination model. In two-dimensional culture, the PCEPS treatment inhibited growth of both murine human cells a dose- time-dependent manner. contrast, splenocytes (SPLs) bone marrow (BMCs) stimulated by with PCEPS. also increased specific subpopulations BMCs: antigen...
A cyclic peptide targeted to the immune checkpoint protein CTLA4 is designed using a hierarchy of computational methods, demonstrated bind by physical measurement, and shown inhibit tumor growth in cell culture mice.
GPCR subtypes possess distinct functional and pharmacological profiles, thus development of subtype-selective ligands has immense therapeutic potential. This is especially the case for angiotensin receptor AT1R AT2R, where a negative control been described AT2R activation highlighted as an important cancer drug target. We describe strategy to fine-tune ligand selectivity AT2R/AT1R through electronic aromatic-prolyl interactions. Through this high affinity (Ki = 3 nM) agonist analogue that...
We have recently discovered the potential involvement of angiotensin II type 2 receptor (AT2R) signaling in pancreatic cancer using AT2R deficient mice. To examine expression human PDAC, expressions as well major (type 1 receptor, AT1R) PDAC and adjacent normal tissue was evaluated by immunohistochemistry real time PCR surgically dissected specimens. In immunohistochemical analysis, relatively strong AT1R detected consistently both pancreas areas, whereas moderate 78.5% specimens 100% area...
Abstract Transfection efficiency and toxicity concerns remain a challenge for gene therapy. Cell-penetrating peptides (CPP) have been broadly investigated to improve the transfection of genetic material (e.g., pDNA siRNA). Here, synthetic CPP (polylysine, K9 peptide) was complexed with angiotensin II type 2 receptor (AT2R) plasmid DNA (pAT2R) complexes were condensed using calcium chloride. The resulting small (∼150 nm) showed high levels expression in vitro vivo. This simple nonviral...
The antitumor effects of a partially purified water extract from Euglena gracilis (EWE) and EWE treated by boiling (bEWE) were evaluated using orthotopic lung cancer syngeneic mouse models with Lewis carcinoma (LLC) cells. Daily oral administration either or bEWE started three weeks prior to the inoculation LLC cells significantly attenuated tumor growth as compared phosphate buffered saline (PBS) control, attenuation was further enhanced bEWE. intestinal microbiota compositions in both...
The partially purified water extract from Euglena gracilis (EWE) was evaluated for its antitumor and immunomodulatory effects in cell cultures a mouse orthotopic lung carcinoma allograft model. In two-dimensional culture, the EWE treatment inhibited growth of both murine Lewis (LLC) human cells (A549 H1299) dose- time-dependent manner. contrast, bone marrow (BMCs), but not splenocytes (SPLs), stimulated by with EWE. three-dimensional spheroid LLC significantly attenuated treatment. model,...
Binding configuration of a de novo stapled peptide on SARS-CoV-2 spike protein, as predicted by molecular simulation. Stapled residues enhance stability while interacting engage key amino acids the protein receptor-binding domain.
A colon cancer growth inhibitor partially purified from the isolated cell wall membrane fraction of Chlorella sorokiniana, here referred to as factor (CMF), was evaluated for its antitumor and immunomodulatory effects in culture a carcinoma mouse model. The CMF treatment dose- time-dependently inhibited 2-dimensional cultures. Treatment with also significantly spheroids 3-dimensional coculture T lymphocytes. In CT26 peritoneal dissemination model, intraperitoneal injection (10 or 30 mg dry...
The migratory ability of chick primordial germ cells (PGCs) transferred into quail embryos was investigated. One, ten, twenty, fifty or one hundred PGCs were the dorsal aorta 2.5-day-old embryos. One day later, isolated, and serial sections prepared after embedding in paraffin. then double-stained with periodic acid-Schiff (PAS) hematoxylin, numbers PAS-positive germinal epithelium, gonadal area, head area trunk determined. Approximately 70% detected 1 transfer, roughly 60% region 10%...
Several crystalline β-D-glycopyranosyl N, N-dialkyldithiocarbamates (sugar=gluco, galacto, xylo or lacto ; alkyl=methyl ethyl) were synthesized by reaction of sodium N-dialkyl-dithiocarbamates with acetylated α-D-glycopyranosyl bromides and sequential deacetylation methanolic ammonia methoxide in methanol. Although the glycosyl dithiocarbamates stable against chilled alkalis which is a marked difference from xanthates isothiuronium, easily decomposed mercury salts to form glycosides under...
Beta glucans are known to have immunomodulatory effects that mediated by a variety of mechanisms. In this article, we describe experiments and simulations suggesting beta-1,3 may promote activation T cells previously unknown mechanism. First, find treatment lymphoblast cell line with oligoglucan significantly increases mRNA levels activation-associated cytokines, especially in the presence agonistic anti-CD3 antibody. This immunostimulatory activity was observed absence dectin-1, receptor...
Interspecies or even inter-genus somatic nuclear transfer is considered to be an effective means for conserving a wide variety of avian genetic resources. However, offspring production currently limited mammals. Therefore, the present experiment was designed in attempt produce transferred gonadal germ cells (snt-GGCs) between chicken and quail. Electrofusion carried out embryonic blood (EBCs) collected from 4-day-old embryos (donor cells) (GGCs) 7-day-old chick 6-day-old quail (recipient...
1, 2-Di-S-acetyl-3, 4, 6-tri-O-acetyl-β-D-mannopyranose (X) and methyl 2-dithio-2-S-methyl-3, 6-tri-O-acetyl-β-D-mannopyranoside (XI) were synthesized using an unequivocal route. A detailed comparison of the physical constants NMR data X with those 2-di-S-acetyl-3, 6-tri-O-acetyl-β-D-mannopyranose, which had been reported from our laboratory (H. Nakamura, S. Tejima, M. Akagi, Chem. Pharm. Bull. (Tokyo), 14, 648 (1966).) clarified that previous compound (VI) was α-anomer X. The corresponding...
A novel peptide that interferes with the PD-1/PD-L1 immune checkpoint pathway, termed PD-L1 inhibitory 3 (PD-L1ip3), was computationally designed, experimentally validated for its specific binding to PD-L1, and evaluated antitumor effects in cell culture a mouse colon carcinoma syngeneic murine model. In several studies, direct treatment PD-L1ip3, but not similar scrambled sequence, substantially increased death of CT26 cells when co-cultured CD8+ T primed by antigens. tumor model, growth...
A water extract derived from the isolated cell walls of Chlorella sorokiniana (C. sorokiniana, extract, CWE) was analyzed for presence lipopolysaccharide (LPS)-related material via Limulus amebocyte lysate (LAL) assay and evaluated its growth stimulation effect on bone marrow cells splenocytes in vitro cultures. The contained low levels LPS-related material, a mass spectrum suggested that many components, including level lipid precursor, compound known as X, which is to elicit positive...