A5073132099- Cancer Immunotherapy and Biomarkers
- Supramolecular Self-Assembly in Materials
- Peptidase Inhibition and Analysis
- Ion channel regulation and function
- Monoclonal and Polyclonal Antibodies Research
- Nerve Injury and Rehabilitation
- Algal biology and biofuel production
- Cancer Cells and Metastasis
- Wastewater Treatment and Nitrogen Removal
- Connexins and lens biology
- SARS-CoV-2 and COVID-19 Research
- Chemical Synthesis and Analysis
- Nanopore and Nanochannel Transport Studies
- Cardiac electrophysiology and arrhythmias
- Electrohydrodynamics and Fluid Dynamics
- Water Quality Monitoring and Analysis
- Graphene research and applications
- Ion Channels and Receptors
- Peripheral Nerve Disorders
- Spectroscopy and Quantum Chemical Studies
- Autoimmune Neurological Disorders and Treatments
- Antimicrobial Peptides and Activities
- Retinal Imaging and Analysis
- Botulinum Toxin and Related Neurological Disorders
- Ocular Diseases and Behçet’s Syndrome
QB3
2024
University of California, Berkeley
2022-2024
Kansas State University
2017-2023
United States Department of State
2021-2022
National Institute of Mental Health and Neurosciences
2013-2016
Johns Hopkins Hospital
2014
Johns Hopkins University
2012
<h3>BACKGROUND AND PURPOSE:</h3> A number of benign and malignant peripheral nerve tumor tumorlike conditions produce similar imaging features on conventional anatomic MR imaging. Functional using DTI can increment the diagnostic performance in differentiation these lesions. Our aim was to evaluate role 3T characterization conditions. <h3>MATERIALS METHODS:</h3> Twenty-nine patients (13 men, 16 women; mean age, 41 ± 18 years; range, 11–83 years) with a or condition (25 benign, 5 malignant)...
Hydration layers are formed on hydrophilic crystalline surfaces immersed in water.
Adsorption of organic molecules from aqueous solution to the surface carbon nanotubes or graphene is an important process in many applications these materials. Here we use molecular dynamics simulation, supplemented by analytical chemistry, explore detail adsorption thermodynamics a diverse set aromatic compounds on graphenic materials, elucidating effects solvent, coverage, curvature, defects, and functionalization hydroxy groups. We decompose free energies into entropic enthalpic...
Superparamagnetic iron oxide nanoparticles (SPIONs) are highly biocompatible and have a versatile synthetic technique based on coprecipitation, reduction-precipitation, hydrothermal methods, where Fe3+ Fe2+ react in aqueous solutions; both these ions present our body clear metabolic pathways; therefore, they attracted extensive research interest development the field of diagnostic imaging therapy. However, most SPION-based clinical contrast agents discontinued due to severe pain, low...
A cyclic peptide targeted to the immune checkpoint protein CTLA4 is designed using a hierarchy of computational methods, demonstrated bind by physical measurement, and shown inhibit tumor growth in cell culture mice.
Enhanced electrical activity in detrusor smooth muscle (DSM) cells is a key factor overactivity which causes overactive bladder pathological disorders. Transient receptor potential melastatin-4 (TRPM4) channels, calcium-activated cation play role regulating DSM activities. These channels likely contribute to depolarizing the cell membrane, leading overactivity. Our research focuses on understanding TRPM4 channel function mouse using computational modeling. We aimed create detailed model of...
The partially purified water extract from Euglena gracilis (EWE) was evaluated for its antitumor and immunomodulatory effects in cell cultures a mouse orthotopic lung carcinoma allograft model. In two-dimensional culture, the EWE treatment inhibited growth of both murine Lewis (LLC) human cells (A549 H1299) dose- time-dependent manner. contrast, bone marrow (BMCs), but not splenocytes (SPLs), stimulated by with EWE. three-dimensional spheroid LLC significantly attenuated treatment. model,...
Binding configuration of a de novo stapled peptide on SARS-CoV-2 spike protein, as predicted by molecular simulation. Stapled residues enhance stability while interacting engage key amino acids the protein receptor-binding domain.
A colon cancer growth inhibitor partially purified from the isolated cell wall membrane fraction of Chlorella sorokiniana, here referred to as factor (CMF), was evaluated for its antitumor and immunomodulatory effects in culture a carcinoma mouse model. The CMF treatment dose- time-dependently inhibited 2-dimensional cultures. Treatment with also significantly spheroids 3-dimensional coculture T lymphocytes. In CT26 peritoneal dissemination model, intraperitoneal injection (10 or 30 mg dry...
Ordered nanoscale patterns have been observed by atomic force microscopy at graphene-water and graphite-water interfaces. The two dominant explanations for these are that (i) they consist of self-assembled organic contaminants or (ii) dense layers formed from atmospheric gases (especially nitrogen). Here we apply molecular dynamics simulations to study the behavior dinitrogen possible interface. Despite high concentration N2 in ambient air, find its expected occupancy interface is quite low....
The graphite-water interface provides a unique environment for polypeptides that generally favors ordered structures more than in solution. Therefore, systems consisting of designed peptides and graphitic carbon might serve as convenient medium controlled self-assembly functional materials. Here, we computationally cyclic spontaneously fold into β-sheet-like conformation at the self-assemble, subsequently observed evidence such assembly by atomic force microscopy. Using novel protocol,...
Two-dimensional fluorescence difference spectroscopy (2-D FDS) was used to determine the unique spectral signatures of zinc oxide (ZnO), magnesium (MgO), and 5% nanocomposite (5% Mg/ZnO) then demonstrate change in signature that occurs when physiologically important proteins, such as angiotensin-converting enzyme (ACE) ribonuclease A (RNase A), interact with ZnO nanoparticles (NPs). When RNase is bound Mg/ZnO, intensity quenched, while magnified a significant shift seen torula yeast RNA...
A 9-y-old, castrated male, domestic medium-hair cat diagnosed previously with chronic kidney disease developed anorexia and vomiting. Ultrasonography revealed abdominal effusion a left renal perihilar mass. Cytologic evaluation of the peritoneal fluid mass identified atypical epithelioid cells suspected to be epithelial or possible mesothelial origin. Immunohistochemical (IHC) formalin-fixed, paraffin-embedded cell block indicated both pancytokeratin vimentin expression in population. With...
A water extract derived from the isolated cell walls of Chlorella sorokiniana (C. sorokiniana, extract, CWE) was analyzed for presence lipopolysaccharide (LPS)-related material via Limulus amebocyte lysate (LAL) assay and evaluated its growth stimulation effect on bone marrow cells splenocytes in vitro cultures. The contained low levels LPS-related material, a mass spectrum suggested that many components, including level lipid precursor, compound known as X, which is to elicit positive...
A novel peptide that interferes with the PD-1/PD-L1 immune checkpoint pathway, termed PD-L1 inhibitory 3 (PD-L1ip3), was computationally designed, experimentally validated for its specific binding to PD-L1, and evaluated antitumor effects in cell culture a mouse colon carcinoma syngeneic murine model. In several studies, direct treatment PD-L1ip3, but not similar scrambled sequence, substantially increased death of CT26 cells when co-cultured CD8+ T primed by antigens. tumor model, growth...
Abstract Proteins involved in immune checkpoint pathways, such as CTLA4, PD-1, and PD-L1, have become important targets for cancer immunotherapy; however, the development of small molecule drugs targeting these pathways has proven difficult due to nature their protein-protein interfaces. Here, using a hierarchy computational techniques, we designed cyclic peptide that binds CTLA4 follow this with experimental verification binding biological activity, bio-layer interferometry, cell culture,...
Enhanced electrical activity in detrusor smooth muscle (DSM) cells is a key factor overactivity which causes overactive bladder pathological disorders. Transient receptor potential melastatin-4 (TRPM4) channels, are calcium-activated cation play role regulating DSM activities. These channels likely contribute to depolarizing the cell membrane, leading overactivity. Our research focuses on understanding TRPM4 channel function of mice, using computational modeling. We aimed create detailed...
Congenital myopathies (CMs) though considered distinct disorders, simultaneous occurrence of central nucleus, nemaline rods, and cores in the same biopsy are scarcely reported.A retrospective reassessment cases diagnosed as CMs to look for multiple pathologies missed, if any, during initial diagnosis.Enzyme histochemical, immunohistochemical-stained slides from 125 congenital myopathy were reassessed.The study revealed 15 (12%) with more than one morphological feature. Central nucleus (n =...