Abstract MicroRNA plays an important role in gastric cancer (GC) development, while the function of miR-497-5p this disease remains unknown. In present study, we demonstrated as a tumor suppressive microRNA GC. was down-regulated GC tissues and its expression associated with stage. Inhibition promoted cell proliferation growth. By contrast, ectopic suppressed growth cells. addition, inhibited DNA synthesis enhanced apoptosis The cycle progression by miR-497-5p. Mechanistically, directly...

The aim of the present study was to investigate neuroprotective effects naringin on memory impairment hydrocortisone mice, and elucidate potential underlying molecular mechanisms. In study, a model constructed. Novel object recognition, Morris water maze step‑down tests were performed in order assess learning abilities mice. Hematoxylin eosin staining used observe pathological changes hippocampus hypothalamus. Transmission electron microscopy ultrastructural hippocampus. Immunohistochemistry...

Objective To investigate the efficacy of PD-L1 blockade in restoring humoral immune response against HBV. Methods HBV‐persistent C57BL/6J mice were established through hydrodynamic tail vein injection 10 µg pAAV‐HBV1.2 plasmid. Subsequently, treated i.p. with anti‐PD‐L1 and/or anti‐CTLA‐4 at specified time points, dosages 500 µg, 250 and respectively. Additionally, 5 × magnetic bead‐purified plasmacytoid dendritic cells (pDCs) adoptively transferred i.v. into acute mouse model followed by...

Paeonol is a major phenolic micromolecular component of Moutan cortex Radicis, traditional Chinese Medicine. It has shown antitumor effects in previous studies; however, the underlying mechanisms remain unknown. This study investigated mechanism by giving treatments placebo, cyclophosphamide, paeonol 150 and 300 mg/kg to 4 groups mice bearing EMT6 breast cancer. Apoptosis tumor cells were confirmed morphology analysis, including hematoxylin, eosin staining TUNEL staining. The results showed...

Abstract Cuproptosis plays an important role in cancer, but its lung cancer remains unknown. Transcriptional profiles, clinical details and mutation data were acquired from the Cancer Genome Atlas database through a variety of methods. The analysis this publicly available was comprehensively performed using R software along with relevant packages, ensuring thorough examination information. In study, we conducted detailed cuproptosis‐related genes lncRNA co‐expression, identifying 129 lncRNAs...

Paeonol, a phenolic component from the root bark of Paeonia moutan, has been identified to possess antitumor effects. However, effect paeonol and mechanism CXCL4/CXCR3-B signals in paeonol-induced breast cancer cell remain unknown.After MDA-MB-231 cells were pretreated with or DMSO, proliferation activity was detected by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), Hoechst, Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), Annexin-V/propidium iodide...

To assess the role of adenosine receptors in regulation coronary microvascular permeability to porcine serum albumin (P(s)(PSA)).Solute flux was measured single perfused arterioles and venules isolated from pig hearts using fluorescent dye-labeled probes by microspectro-fluorometry. Messenger RNA, protein, cellular distribution were analyzed RT-PCR, immunoblot, immunofluorescence.Control venule P(s)(PSA) (10.7 +/- 4.8 x 10(- 7) cm s(- 1)) greater than that (6.4+/- 2.8 10(-7) . s(-1); p <...

Abstract Objectives Human lactoferrin, an 80 kDa iron-binding glycoprotein, has antitumour effects. We have explored the potential therapeutic role of re-expressing human lactoferrin gene product in breast cancer. Methods A recombinant adenovirus expressing cDNA (ad-hLTF) was constructed and used to infect cancer cells. Key findings Seventy-two hours after infection, ad-hLTF had considerable cytotoxicity on MCF-7 time-course study showed that infection cells at 100 plaque-forming units per...

Two-dimensional fluorescence difference spectroscopy (2-D FDS) was used to determine the unique spectral signatures of zinc oxide (ZnO), magnesium (MgO), and 5% nanocomposite (5% Mg/ZnO) then demonstrate change in signature that occurs when physiologically important proteins, such as angiotensin-converting enzyme (ACE) ribonuclease A (RNase A), interact with ZnO nanoparticles (NPs). When RNase is bound Mg/ZnO, intensity quenched, while magnified a significant shift seen torula yeast RNA...

Acute pulmonary embolism (PE) remains a significant cause of cardiovascular morbidity and mortality worldwide. Brain natriuretic peptide (BNP) combined with catheter-directed therapy (CDT) may improve right ventricular (RV) dysfunction stabilize hemodynamics in acute PE.We retrospectively studied 159 patients confirmed PE who were treated CDT admitted to the intensive care unit our department between September 2016 May 2020. The divided into control group rhBNP based on whether receive...

The sex of an animal impacts glucose sensitivity, but little information is available regarding the mechanisms causing that difference, especially during acute inflammation. We examined sex-specific differences in role P2Y 2 receptor (P2Y R) flux with and without LPS challenge. Male female wild-type R knockout mice -/- ) were injected or saline tolerance tests (GTT) performed. R, insulin receptor, GLUT4 transporter gene expression was also evaluated. Female had reduced fasting plasma females...

Oleanolic acid (OA) is a natural compound that can be found in number of edible and medicinal plants confers diverse biological actions. However, the direct target OA human tumor cells remains poorly understood, preventing its application clinical health settings. A previous study revealed overexpression caveolin‑1 leukemia HL‑60 increase sensitivity to OA. The present aimed investigate effects on doxorubicin‑resistant breast cancer MCF‑7 cell line (MCF‑7/DOX), harringtonine‑resistant...

That P2Y2 nucleotide receptor (P2Y2R) is critical to endothelial‐dependent inflammatory vascular responses including leukocyte recruitment and filtration was revealed by abolishment of in P2Y2R‐deficient mice. The role P2Y2R the mediation microvascular permeability, however, remains be investigated. Both vivo vitro approaches were used test hypothesis that activation would increase permeability. We measured cremaster venular permeability albumin ( P s ) response UTP, a agonist, wild type...

Interleukin (IL)‑32 is induced by pro‑inflammatory cytokines and promotes the release of inflammatory cytokines. Therefore, it can promote responses. The present review article summarized role receptors required for IL‑32 action, biological function its mechanism action in tumors. Moreover, assessed significance aberrant expression associated diseases analyzed effects on four key types cancer: Colorectal, gastric, breast lung. However, needs to be further demonstrated assessing this cytokine...

Objective To observe the expression rate and average fluorescence intensity of receptor activator NF-kB (RANK), RANK ligand (RANKL) , Osteoprotegerin (OPG) CD3~+ T lymphocytes, neu-trophils, CD14 monocyte in peripheral blood rheumatoid arthritis (RA) model rats to explore significance bone destruction inflammation process RA by using Ligustrazine. Methods Rats were randomly divided into five groups, normal group, control high dosage Ligustrazine low group positive drug group. Each had ten...

We found that stimulation of P2Y 2 receptor (P2Y R) induced transient increase in venule permeability as 5‐folds greater than baseline C57BL mouse cremaster skeletal muscle. Given integrin mediates the signaling, we hypothesized focal adhesion complex would be involved signaling pathway to regulate R‐induced hyperpermeability. To test hypothesis, isolated and cultured microvascular endothelial cells derived from both wild‐type mice R knockout vitro models assess kinase mechanism underlying...

The adenosine (ADO) A3 receptors on mast cells have been shown to mediate ADO-induced arteriolar vasoconstriction and increase in cutaneous vascular permeability. To determine whether a similar role exists for ADO the regulation of permeability responses arterioles venules isolated from juvenile female rat skeletal muscle, albumin (Ps) was determined duringADO (10−5 M) suffusion without with selective receptor antagonist, MRS1191 (10−7 M). Consistent mediation Ps response ADO, abolished...