A5044033298- Retinal Development and Disorders
- Cellular Mechanics and Interactions
- Zebrafish Biomedical Research Applications
- Advanced Fluorescence Microscopy Techniques
- Gene Regulatory Network Analysis
- Cell Image Analysis Techniques
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Genomics and Phylogenetic Studies
- Microtubule and mitosis dynamics
- Virus-based gene therapy research
- Nuclear Structure and Function
- Computational Drug Discovery Methods
- Plant Virus Research Studies
- Bipolar Disorder and Treatment
- Cellular transport and secretion
- Amino Acid Enzymes and Metabolism
- Cell death mechanisms and regulation
- Bacteriophages and microbial interactions
- Adenosine and Purinergic Signaling
- Endoplasmic Reticulum Stress and Disease
- CRISPR and Genetic Engineering
University of Cambridge
2017-2020
University of Ottawa
2012-2019
Ottawa Institute of Systems Biology
2015-2016
Local translation regulates the axonal proteome, playing an important role in neuronal wiring and axon maintenance. How mRNAs are localized to specific subcellular sites for translation, however, is not understood. Here we report that RNA granules associate with endosomes along axons of retinal ganglion cells. RNA-bearing Rab7a late also ribosomes, real-time imaging reveals they local protein synthesis. We show often pause on mitochondria encoding proteins mitochondrial function translated...
An important question in early neural development is the origin of stochastic nuclear movement between apical and basal surfaces neuroepithelia during interkinetic migration. Tracking subpopulations has shown evidence diffusion - mean squared displacements growing linearly time suggested crowding from cell division at surface drives basalward motion. Yet, this hypothesis not yet been tested, forces involved quantified. We employ long-term, rapid light-sheet two-photon imaging zebrafish...
The world of health care has witnessed an explosive boost to its capacity within the past few decades due introduction viral therapeutics medicinal arsenal. As a result, need for new methods quantification arisen accommodate this rapid advancement in virology and associated requirements efficiency, speed, quality control. In work, we apply quantitative capillary electrophoresis (viral qCE) determine (i) number intact virus particles (ivp) samples, (ii) amount DNA contamination, (iii) degree...
Drug resistance complicates the treatment of cancer and infectious diseases, often arises from elevated expression a gene that neutralises or reduces drug activity. To investigate this other expression-based mechanisms resistance, authors engineered set regulatory networks in eukaryotic model organism Saccharomyces cerevisiae to control homologue cancer-related human multidrug MDR1. Using system, they explored experimentally how different network features, also called genetic motifs,...
The Registry of Standard Biological Parts imposes sequence constraints to enable DNA assembly using restriction enzymes. Alnahhas et al. (Journal Engineering 2014, 8:28) recently argued that these should be revised because they impose an unnecessary burden on contributors use homology-based assembly. To add this debate, we tested four different methods, and found students methods their first attempt have a high probability success. Because ease success rates, believe is best practice...
Abstract A major hallmark of neural development is the oscillatory movement nuclei between apical and basal surfaces neuroepithelium during process interkinetic nuclear migration (IKNM). Here, we employ long-term, rapid lightsheet two-photon imaging Zebrafish retinas in vivo early to uncover physical processes that govern behavior IKNM. These images allow capture reliable tracks movements division retinogenesis for many tightly packed nuclei. are then used create test a theory retinal IKNM...
A major hallmark of neural development is the oscillatory movement nuclei between apical and basal surfaces neuroepithelium during process interkinetic nuclear migration (IKNM). Here, we employ long-term, rapid lightsheet two-photon imaging zebrafish retinas $in~vivo$ early to uncover physical processes that govern behavior IKNM. These images allow capture reliable tracks movements division retinogenesis for many tightly packed nuclei. are then used create test a theory retinal IKNM as...